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Apoptotic and anti-angiogenic effects of Salvia triloba extract in prostate cancer cell lines

  • Original Article
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Tumor Biology

Abstract

Plants, due to their remarkable composition, are considered as natural resources of bioactive compounds with specific biological activities. Salvia genus (Lamiaceae) has been used around the world in complementary medicine since ancient times. We investigated the cytotoxic, apoptotic and anti-angiogenic effects of methanolic Salvia triloba extract (STE) in prostate cancer cells. Cell viability was evaluated by XTT; apoptosis was investigated by DNA fragmentation and caspase 3/7 activity assays. Changes in the angiogenic cytokine levels were investigated by human angiogenesis antibody array. Scratch assay was used to determine the cell motility. STE induced cytotoxicity and apoptosis in a concentration-dependent manner in both cancer cells; however, it was not cytotoxic to normal cells. Cell motility was reduced in PC-3, DU-145 and HUVEC cells by STE treatment. ANG, ENA-78, bFGF, EGF, IGF-1 and VEGF-D levels were significantly decreased by −2.9, −3.7, −1.7, −1.7, −2.0 and −1.8 fold in STE-treated DU-145 cells, however, ANG, IL-8, LEP, RANTES, TIMP-1, TIMP-2 and VEGF levels were significantly decreased by −5.1, −2.0, −2.4, −3.1, −1.5, −2.0 and −2.5 fold in PC-3 cells. These data suggest that STE might be a promising candidate for anti-tumor and anti-angiogenic treatment of prostate cancer.

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Acknowledgments

This research was done at Medical Oncology Research Laboratories, School of Medicine, Tulay Aktas Oncology Hospital, Ege University. We thank Naturin Natural Products Ltd. (Izmir, Turkey) for supplying the plant material.

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  1. Section of Molecular Biology, Department of Biology, Faculty of Science and Letters, Celal Bayar University, 45140, Muradiye, Manisa, Turkey

    Harika Atmaca & Emir Bozkurt

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  1. Harika Atmaca
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  2. Emir Bozkurt
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Correspondence to Harika Atmaca.

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Atmaca, H., Bozkurt, E. Apoptotic and anti-angiogenic effects of Salvia triloba extract in prostate cancer cell lines. Tumor Biol. 37, 3639–3646 (2016). https://doi.org/10.1007/s13277-015-4208-2

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  • DOI: https://doi.org/10.1007/s13277-015-4208-2

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