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Production of an exopolysaccharide by Antarctic yeast

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Abstract

Psychrophilic Antarctic yeasts produce polysaccharides in different concentrations. According to morphological, cultural, physiological and biochemical characteristics, the best producer strain was identified as Cryptococcus flavus A51. The highest values for viscosity (59.1 mPa s) and crude polysaccharide productivity (5.75 g/L) were obtained in a medium containing 5 % sucrose and 0.25 % (NH4)2SO4, at 24 °C for 6 d. The chemical composition and sugar constituents of the crude exopolysaccharide were determined (92.5 % saccharides, 3.34 % protein, and 4.16 % ash). The monosaccharide composition of the exopolysaccharide obtained from C. flavus strain AL51 was established (55.1 % mannose, 26.1 % glucose, 9.60 % xylose, 1.90 % galactose). The microbial biopolymer has a high molar mass and homogeneity: 82 % of it had M 1.01 MDa.

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Abbreviations

DBB:

diazonium blue B (a test)

HPSEC:

high-performance size-exclusion chromatography

EPS:

exopolysaccharide(s)

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Authors and Affiliations

  1. Laboratory of Applied Microbiology, Institute of Microbiology, Bulgarian Academy of Sciences, 4002, Plovdiv, Bulgaria

    K. Pavlova

  2. University of Food Technologies, 4002, Plovdiv, Bulgaria

    I. Panchev

  3. Institute of Organic Chemistry with Phytochemistry Centre, Bulgarian Academy of Sciences, P.O. Box 27, 4002, Plovdiv, Bulgaria

    M. Krachanova & M. Gocheva

Authors
  1. K. Pavlova
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  2. I. Panchev
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  3. M. Krachanova
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  4. M. Gocheva
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Correspondence to K. Pavlova.

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Pavlova, K., Panchev, I., Krachanova, M. et al. Production of an exopolysaccharide by Antarctic yeast. Folia Microbiol 54, 343–348 (2009). https://doi.org/10.1007/s12223-009-0049-y

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  • DOI: https://doi.org/10.1007/s12223-009-0049-y

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