Skip to main content
Log in

Lipid composition of some yeast strains from Livingston Island, Antarctica

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Yeast strainsCryptococcus albidus, Cryptococcus laurentii, Rhodotorula minuta were isolated from a moss sample,Candida oleophila andRhodotorula mucilaginosa were isolated from a soil sample taken from Livingston Island. Antarctica. Fatty acid, phospholipid, sterol and tocopherol composition was determined in separated lipid fraction after fermentation in a medium containing glucose, peptone and yeast extract. Unsaturated fatty acids, mainly oleic (51–65%) and linoleic (9.5–16.8%), predominated in triacylglycerols. Sterols represent ca. 120–930 mg per kg dry biomass. The content of major phospholipids (phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine) was ca. 100–800 mg/kg. The amount of tocopherols (mainly γ- and δ-tocopherol) was 2.1–6.3 mg/kg.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aggelis G., Stathas D., Tavonlaris N., Komaitis M.: Composition of lipids by some strains ofCandida species. Production of single cell oil in a chemostat culture.Folia Microbiol.41, 299–302 (1966).

    Google Scholar 

  • Akhtar P., Gray J.I., Asghar A.: Synthesis of lipids by certain yeast strains grown on whey permeate.J. Food Lipids5, 283–297 (1998).

    Article  CAS  Google Scholar 

  • Akhtar P., Gray J.I., Asghar A.: Chemical characterization and stereospecific analysis of lipids synthesized by certain yeast strains.J. Food Lipids5, 299–311 (1998).

    Article  CAS  Google Scholar 

  • Arthur H., Watson K.: Thermal adaptation in yeast: growth temperatures, membrane lipid and cytochrome composition of psychrophilic, mesophilic and thermophilic yeasts.J. Bacteriol.128, 56–68 (1976).

    PubMed  CAS  Google Scholar 

  • Augustyn O.P.H., Kock J.F.: Differentiation of yeast species and strains within a species, by cellular fatty acid analysis. 1. Application of an adapted technique to differentiate between strains ofSaccharomyces cerevisiae.J. Microbiol. Meth.10, 9–23 (1989).

    Article  CAS  Google Scholar 

  • Beshkov M., Ivanova L.: Determination of phospholipids in lipid mixtures.Sci. Works High Inst. Food Flavor Ind. Plovdiv (Bulgaria)20, 231–234 (1972).

    Google Scholar 

  • Celligoi M.C., Angelia D.F., Buzato J.B.: Application of sugar-cane molasses in the production of lipids by yeast.Agr. Biol. Technol.40, 693–698 (1997).

    CAS  Google Scholar 

  • Folch M., Kees M., Stanley G.: A simple method for the isolation and purification of total lipids from animal tissues.J. Biol. Chem.226, 497–498 (1957).

    PubMed  CAS  Google Scholar 

  • Ivanov S., Aitzetmüller K.: Untersuchungen über die Tocopherol- und Tocotrienolzusammensetzung der Samenöle einiger Vertreter der FamilieApiaceae.Fat. Sci. Technol.97, 24–29 (1995).

    CAS  Google Scholar 

  • Ivanov S., Bitcheva P., Konova B.: Des phytosterols dans les huiles vegetales et les concentres steroliques.Rev. Fr. Corps. Gras19, 177–180 (1972).

    CAS  Google Scholar 

  • Jacob Z.: Yeast lipid biotechnology.Adv. Appl. Microbiol.39, 185–212 (1993).

    Article  PubMed  CAS  Google Scholar 

  • Kates M.:Technique of Lipidology. American Elsevier Publishers, New York 1972.

    Google Scholar 

  • Kock J.L.F., Lategan P.M., Botes P.J., Viljoen B.C.: Developing a rapid statistical identification process for different yeast species.J. Microbiol. Meth.4, 3–4 (1985).

    Article  Google Scholar 

  • Kurtzman C.P., Fell J.W.:The Yeasts: a Taxonomic Study, 4th ed. Elsevier Scientific Publishers, Amsterdam (Netherlands) 1998.

    Google Scholar 

  • Lyapkov B.G., Kiseleva T.V.: Characterization of microbial composition and their comparison with vegetable oils.Appl. Biochem. Microbiol.29, 120–123 (1993).

    Google Scholar 

  • Metcalee L., Wang C.: Rapid preparation of fatty acid methyl esters using organic base catalyzed transesterification.J. Chromatogr. Sci.19, 530–534 (1981).

    Google Scholar 

  • Nojoma Y., Kibayashi A., Matsuzaki H., Hatano T., Fukui S.: Isolation and characterization of triacylglycerol-secreting mutant strain from yeastSaccharomyces cerevisiae.J. Gen. Appl. Microbiol.45, 1–6 (1999).

    Article  Google Scholar 

  • Pavlova K., Grigorova D., Gushterova A.: Preliminary investigation of the isolation of yeasts from the Livingston Island (the Antarctic).Bulgarian Antarctic Res Life Sci.2, 110–114 (1999).

    Google Scholar 

  • Pavlova K., Grigorova D., Hristozova T., Angelov A.: Yeast strains from the Livingston Island, Antarctica.Folia Microbiol.46, 397–401 (2001).

    CAS  Google Scholar 

  • Ratledge C.: Microbiol fats and oils: an assessment potential.Progr. Industr. Microbiol.16, 119–206 (1982).

    CAS  Google Scholar 

  • Ratledge C.: Yeasts for lipid production.Biochem. Soc. Trans.16, 1088–1091 (1988).

    PubMed  CAS  Google Scholar 

  • Ratledge C., Boulton C.A.: Fats and oils, pp. 984–1003 in C.L. Cooneg, A.E. Humphrey (Eds.):Comprehensive Biotechnology, Vol. 3. Pergamon Press, Oxford 1985.

    Google Scholar 

  • Řezanka T., Rozentsvet O.A., Dembitsky V.M.: Characterization of the hydroxy fatty acid content ofBasidiomycotina.Folia Microbiol.44, 635–642 (1999).

    Google Scholar 

  • Saxena V., Sharma C.D., Bhagat S.D., Saine V.S., Adhikari D.K.: Lipid and fatty acid biosynthesis byRhodotorula minuta.J. Am. Offic. Chem. Soc.75, 501–505 (1998).

    Article  CAS  Google Scholar 

  • Smith E.J., Westhuizen J.P.J., Kock J.L.F., Lategan P.M.: A yeast identification method: the influence of culture age on the cellular long-chain fatty acid. Composition of three selected basidiomycetous yeast.Syst. Appl. Microbiol.10, 38–41 (1987).

    CAS  Google Scholar 

  • Vishniac H.C.: Psychrophilic yeasts, pp. 315–321 in J. Seckbach (Ed.),Enigmatic Microorganisms and Life in Extreme Environments. Kluwer Academic Publishers, Dordrecht (Netherlands) 1999.

    Google Scholar 

  • Vishniac H.S., Kurtzman C.P.:Cryptococcus antarcticus sp.nov. andCryptococcus albidosimilis sp.nov., basidioblastomycetes from Antarctic soils.Internat. J. Syst. Bacteriol.42, 547–553 (1992).

    Article  Google Scholar 

  • Watson K., Arthur H., Shipton W.A.:Lencosporidium yeasts: obligate psychrophiles which alter membrane-lipid and cytochrome composition with temperature.J. Gen. Microbiol.97, 11–18 (1976).

    PubMed  CAS  Google Scholar 

  • Ymauchi H., Mori H., Kobayashi T., Shimizu S.: Mass production of lipid byLipomyces starkeyii in micro computer aided fedbatch culture.J. Ferment. Technol.61, 275–280 (1983).

    Google Scholar 

  • Ykema A., Kartar M.M., Smith H.: Lipid production in whey permeate by an unsaturated fatty acid mutant of the oleaginous yeastApiotrichum curvatum.Biotechnol. Lett.11, 477–482 (1989).

    Article  CAS  Google Scholar 

  • Zohri A.A.: Progesterone transformation as a biochemical aid in classification of the genusEmericella.Folia Microbiol.45, 391–396 (2000).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Zlatanov.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zlatanov, M., Pavlova, K. & Grigorova, D. Lipid composition of some yeast strains from Livingston Island, Antarctica. Folia Microbiol 46, 402–406 (2001). https://doi.org/10.1007/BF02814429

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02814429

Keywords

Navigation