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LC with Evaporative Light-Scattering Detection for Quantitative Analysis of Organic Acids in Juices

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Abstract

In this work, a performing LC method with evaporative light-scattering detector was described for the analysis of tartaric, malic, ascorbic, citric and succinic acids in fruit juices. The method was optimized and validated in comparison with LC-UV, in terms of accuracy, LODs, LOQs and precision. The successive application to ACE, orange, pear, peach, mulberry and apple juices allowed separation and quantitative determination of organic acids in about 20 min just diluting and filtering the sample before LC determination. In the analysed juices, citric, malic and ascorbic acids were always present, and in all the samples, citric acid was the prevailing acid followed by malic acid except for peach and apple juices where an opposite trend was found. Tartaric and succinic acids were detected at lower concentrations and more rarely, except for peach juices, all containing tartaric acid, and ACE and orange juices, all containing succinic acid.

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Authors and Affiliations

  1. Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende, Italy

    Donatella Restuccia, U. Gianfranco Spizzirri, Francesco Puoci & Nevio Picci

  2. Department of Agricultural and Environmental Sciences, University of Bari, Bari, Italy

    Maria Lisa Clodoveo

Authors
  1. Donatella Restuccia
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  2. U. Gianfranco Spizzirri
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  3. Francesco Puoci
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  4. Maria Lisa Clodoveo
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  5. Nevio Picci
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Correspondence to Donatella Restuccia.

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Funding

This work was financially supported by university funds (ex 60 %).

Conflict of Interest

Donatella Restuccia has received research funds from the University of Calabria.

Umile Gianfranco Spizzirri declares that he has no conflict of interest.

Francesco Puoci has received research funds from the University of Calabria.

Maria Lisa Clodoveo has received research funds from the University of Bari.

Nevio Picci has received research funds from the University of Calabria.

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Restuccia, D., Spizzirri, U.G., Puoci, F. et al. LC with Evaporative Light-Scattering Detection for Quantitative Analysis of Organic Acids in Juices. Food Anal. Methods 10, 704–712 (2017). https://doi.org/10.1007/s12161-016-0628-x

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  • DOI: https://doi.org/10.1007/s12161-016-0628-x

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