Abstract
A simple, sensitive, and rapid method was developed for the routine identification and quantification of menbutone in different matrices by micellar liquid chromatography. Separation was performed in less than 4 min using a C18 column with UV detection at 234 nm. A micellar solution composed of 0.12 M sodium dodecyl sulfate, 8 % n-butanol, and 0.3 % triethylamine in 0.02 M phosphoric acid at pH 6.0 was used as the mobile phase. The method was fully validated in accordance with International Conference on Harmonization (ICH) guidelines. The limits of detection and quantitation were 0.95 and 2.86 ng mL−1, respectively. The method showed good repeatability, linearity, and sensitivity according to the evaluation of the validation parameters. The micellar method was successfully applied for the analysis of menbutone in its commercial injections with a mean % recovery value of 99.73 ± 1.634 % and in spiked bovine milk and meat samples with a mean % recovery values in the range of 98.00–100.60 %. High extraction efficiency was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. No organic solvent was used during the pretreatment step. Hence, this method can be considered as an interesting example for green chemistry.
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15 December 2017
The original version of this article unfortunately contained a mistake. Affiliation 4 should be included for Manal M. Fouad.
References
Ackerman L (2007) Veterinary practice management. Black Well, New Jersey, p 342
Belal F, El-Brashy A, Eid M, Nasr J (2009) Liq Chromatogr Related Technol 32:2993
Belal F, Sharaf El-Din M, El-Enany N, Saad S (2013) Chem Cent J 7:162
Beltran-Martinavarro B, Peris-Vicente J, Marco-Peiro S, Esteve-Romero S, Rambla-Alegre M, Carda-Boch S (2012) Analyst 137:269
Berthod A, Garcia-Alvarez-Coque MC (2000) 83, Marcel Dekker, New York
CX/RVDF 06/16/13, Report on 16th session of the Codex Committee on Residues of Veterinary Drugs in Foods (2006) Codex Alimentarius Commission, Jiont FAO/WHO Food Standards Programme, Rome. http://www.codexalimentarius.net/web/archives.jsp?year=06. Accessed 12 May 2006
Fouad M, Abdel-Razeq S, Belal F, Fouad F (2013) Int J Pharm Anal 4:30
Hirosh M, Kouhei F, Toshiaki T (2010) Kumamoto-Ken Hoken Kankyo Kagaku Kenkyushoho 39:21
International Conference on Harmonization (ICH) (2005) Technical requirements for the registration of pharmaceutical for human use: validation of analytical procedures, text and methodology Q2 (R1), Geneva, 1
Kalyankar T, Kulkarini P, Wadher S, Pekamwar S (2014) J Appl Pharm Sci 4:128
Lund J, Lassen J (1969) Acta Pharmacol Toxicol 27:429
Luo Y, Tan M, Luo L, Wang L, Yang Y, Long F, Wang S, Wu M (2013a) Faming Zhuanli Shenqing CN 103424491 A 12:4
Luo Y, Tan M, Luo L, Wang L, Yang Y, Long F, Wang S, Wu M (2013b) Faming Zhuanli Shenqing CN 103439421 A 12:11
Malinowska I, Stepnik K (2012) Chromatogr Res Int 2012:1
Miller J, Miller J (2005) Statistics and chemometrics for analytical chemistry, 5th edn. Pearson Education Limited, Harlow, p 256
Mourya SK, Dubey S, Durgabanshi A, Shukia SK, Esteve-Romero J, Bose D (2011) J AOAC Int 94:1082
Nasr J, Shalan S, Belal F (2013a) Chromatographia 76:523
Nasr J, Shalan S, Belal F (2013b) Food Anal Methods 6:1522
O’Neil J (2006) The Merck Index, 14th edn. Merck and Co, Inc, Rahway, p 1008
Rambla-Alegre M (2012) Chromatogr Res Int 2012:1
Rizk M, Toubar S, Abou El-Alamin M (2014) Eur J Chem 5:11
Rodriguez E, Moreno-Bondi MC, Marazuela MD (2008) J Chromatogr A 1209:136
Shoichiro N, Hisaya T (2008) Nagoya-shi Eisei Kenkyushoho 54:1
Soltani S, Jouyban A (2012) Bioanalysis 4:41
Symonds HW (1982) Vet Rec 110:423
Szymanski A (2008) Toxicol Mech Methods 18:473
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The authors declare no conflict of interest. This article does not contain any studies with human or animal subjects.
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A correction to this article is available online at https://doi.org/10.1007/s12161-017-1120-y.
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Belal, F., El-Razeq, S.A.A., Fouad, M.M. et al. Determination of Menbutone in Bovine Milk and Meat Using Micellar Liquid Chromatography: Application to Injectable Dosage Forms. Food Anal. Methods 9, 638–645 (2016). https://doi.org/10.1007/s12161-015-0230-7
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DOI: https://doi.org/10.1007/s12161-015-0230-7