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Preferential Promotion of Lycopersicon esculentum (Tomato) Growth by Plant Growth Promoting Bacteria Associated with Tomato

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Abstract

A total of 74 morphologically distinct bacterial colonies were selected during isolation of bacteria from different parts of tomato plant (rhizoplane, phylloplane and rhizosphere) as well as nearby bulk soil. The isolates were screened for plant growth promoting (PGP) traits such as production of indole acetic acid, siderophore, chitinase and hydrogen cyanide as well as phosphate solubilization. Seven isolates viz., NR4, NR6, RP3, PP1, RS4, RP6 and NR1 that exhibited multiple PGP traits were identified, based on morphological, biochemical and 16S rRNA gene sequence analysis, as species that belonged to four genera Aeromonas, Pseudomonas, Bacillus and Enterobacter. All the seven isolates were positive for 1-aminocyclopropane-1-carboxylate deaminase. Isolate NR6 was antagonistic to Fusarium solani and Fusarium moniliforme, and both PP1 and RP6 isolates were antagonistic to F. moniliforme. Except RP6, all isolates adhered significantly to glass surface suggestive of biofilm formation. Seed bacterization of tomato, groundnut, sorghum and chickpea with the seven bacterial isolates resulted in varied growth response in laboratory assay on half strength Murashige and Skoog medium. Most of the tomato isolates positively influenced tomato growth. The growth response was either neutral or negative with groundnut, sorghum and chickpea. Overall, the results suggested that bacteria with PGP traits do not positively influence the growth of all plants, and certain PGP bacteria may exhibit host-specificity. Among the isolates that positively influenced growth of tomato (NR1, RP3, PP1, RS4 and RP6) only RS4 was isolated from tomato rhizosphere. Therefore, the best PGP bacteria can also be isolated from zones other than rhizosphere or rhizoplane of a plant.

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Acknowledgments

We thank the International Atomic Energy Agency (IAEA) for a research grant to ARP. SD, VRVNR, SK, SR and VPR thank Department of Biotechnology (DBT), Government of India (GoI) for a post doctoral or research fellowship. We also thank the DBT-CREBB, DST-FIST and UGC-CAS programmes that provided infrastructure for this work. We thank Dr. Anil Singh for inputs in this work.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, AP, India

    Papa Rao Vaikuntapu, Swarnalee Dutta, Ram Babu Samudrala, Vukanti R. V. N. Rao, Sadaf Kalam & Appa Rao Podile

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  1. Papa Rao Vaikuntapu
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  2. Swarnalee Dutta
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  3. Ram Babu Samudrala
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  6. Appa Rao Podile
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Correspondence to Appa Rao Podile.

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Vaikuntapu, P.R., Dutta, S., Samudrala, R.B. et al. Preferential Promotion of Lycopersicon esculentum (Tomato) Growth by Plant Growth Promoting Bacteria Associated with Tomato. Indian J Microbiol 54, 403–412 (2014). https://doi.org/10.1007/s12088-014-0470-z

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