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Gene structure, phylogeny and expression profile of the sucrose synthase gene family in cacao (Theobroma cacao L.)

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Abstract

In higher plants, sucrose synthase (Sus, EC 2.4.1.13) is widely considered as a key enzyme involved in sucrose metabolism. Although, several paralogous genes encoding different isozymes of Sus have been identified and characterized in multiple plant genomes, to date detailed information about the Sus genes is lacking for cacao. This study reports the identification of six novel Sus genes from economically important cacao tree. Analyses of the gene structure and phylogeny of the Sus genes demonstrated evolutionary conservation in the Sus family across cacao and other plant species. The expression of cacao Sus genes was investigated via real-time PCR in various tissues, different developmental phases of leaf, flower bud and pod. The Sus genes exhibited distinct but partially redundant expression profiles in cacao, with TcSus1, TcSus5 and TcSus6, being the predominant genes in the bark with phloem, TcSus2 predominantly expressing in the seed during the stereotype stage. TcSus3 and TcSus4 were significantly detected more in the pod husk and seed coat along the pod development, and showed development dependent expression profiles in the cacao pod. These results provide new insights into the evolution, and basic information that will assist in elucidating the functions of cacao Sus gene family.

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Acknowledgements

This work was financially supported by the National Science Foundation of China (31301389) and the Tropical Crop Germplasm Resources Protection Project of the Ministry of Agriculture (2015NWB046).

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  1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, Wanning, 571533, People’s Republic of China

    FUPENG LI, CHAOYUN HAO, LIN YAN, BAODUO WU, XIAOWEI QIN, JIANXIONG LAI & YINGHUI SONG

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[Li F., Hao C., Yan L., Wu B., Qin X., Lai J. and Song Y. 2015 Gene structure, phylogeny and expression profile of the sucrose synthase gene family in cacao (Theobroma cacao L.). J. Genet. 94, xx–xx]

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LI, F., HAO, C., YAN, L. et al. Gene structure, phylogeny and expression profile of the sucrose synthase gene family in cacao (Theobroma cacao L.). J Genet 94, 461–472 (2015). https://doi.org/10.1007/s12041-015-0558-1

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