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Association mapping for partial resistance to Phytophthora sojae in soybean (Glycine max (L.) Merr.)

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Abstract

Association mapping is a powerful high-resolution mapping tool for complex traits. The objective of this study was to identify QTLs for partial resistance to Phytophthora sojae. In this study, we evaluated a total of 214 soybean accessions by the hypocotyl inoculation method, and 175 were susceptible. The 175 susceptible accessions were then evaluated for P. sojae partial resistance using slant board assays. The 175 accessions were screened with 138 SSR markers that generated 730 SSR alleles. A subset of 495 SSR loci with minor allele frequency (MAF) ≥ 0.05 was used for association mapping by the Tassel general linear model (GLM) and mixed linear model (MLM) program. This soybean population could be divided into two subpopulations and no or weak relatedness was detected between pairwise accessions. Four SSR alleles, Satt634-133, Satt634-149, Sat_222-168 and Satt301-190, associated with partial resistance to P. sojae were detected by both GLM and MLM methods. Of these identified markers, one marker, Satt301, was located in regions where P. sojae resistance QTL have been previously mapped using linkage analysis. The identified markers will help to understand the genetic basis of partial resistance, and facilitate future marker-assistant selection aimed to improve resistance to P. sojae and reduce disease-related mortality in soybean.

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Acknowledgements

We thank Prof. Li-Juan Qiu (Chinese Academy of Agricultural Sciences) for providing the mini core collection of cultivated soybean in China and Prof. Yuan-Chao Wang (Nanjing Agricultural University) for providing the isolates of P. sojae race 2. This work was supported by the National Basic Research Programme of China was supported by the National Basic Research Programme of China Public Interest (CARS-004-PS10), Jiangsu Provincial Natural Science Foundation (BK2011637), China Transgenic Research Program (2011ZX08004-002) and the fundamental Research Funds for the Central Universities-the Science and Technology Innovation Fund for Young Scholars of Nanjing Agricultural University (KJ2012001).

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Authors and Affiliations

  1. National Center for Soybean Improvement / National Key Laboratory of Crop Genetics and Germplasm Enhancement / Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    JUTAO SUN, NA GUO, JUN LEI, LIHONG LI, GUANJUN HU & HAN XING

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  1. JUTAO SUN
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  2. NA GUO
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  3. JUN LEI
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  4. LIHONG LI
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Correspondence to HAN XING.

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[Sun J., Guo N., Lei J., Li L., Hu G. and Xing H. 2014 Association mapping for partial resistance to Phytophthora sojae in soybean (Glycine max (L.) Merr.). J. Genet. 93, xx–xx]

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SUN, J., GUO, N., LEI, J. et al. Association mapping for partial resistance to Phytophthora sojae in soybean (Glycine max (L.) Merr.). J Genet 93, 355–363 (2014). https://doi.org/10.1007/s12041-014-0383-y

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