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A miRNA-binding site single nucleotide polymorphism in the 3′-UTR region of the NOD2 gene is associated with colorectal cancer

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Abstract

Colorectal cancer (CRC) is one of the common malignancies worldwide. Single nucleotide polymorphisms in miRNA-binding site on gene transcripts are reported to play important role in increased risk of CRC in different population. We performed a case–control study using 88 CRC patients and 88 non-cancer counterparts to evaluate the association between NOD2 rs3135500 polymorphism located at 3′ untranslated region of the gene and risk of sporadic CRC. Genotyping of rs3135500 polymorphism was performed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) assay. We found a significant association of AA genotype with risk of CRC (adjusted OR 3.100, CI 1.621–5.930, p < 0.001). Also, significant difference in physical activity (p = 0.001) between case and control groups was found. We also found that individuals in control group were more aspirin or NSAID user compared to sporadic CRC cases (p = 0.002). In the case group, individuals with GG genotype consumed more aspirin or NSAID compared with AA+AG genotypes (33.3 vs. 9.6 %, adjusted OR 4.71, CI 1.25–17.76, p = 0.02). However, in the control group, individuals with AA+AG genotypes used more aspirin or NSAID compared with GG genotypes (47.2 vs. 11.4 %, adjusted OR 14 %, CI 0.05–0.47, p < 0.001).

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Acknowledgments

Authors are grateful to the Vice Chancellor for Research, Isfahan University of Medical Sciences, for financial support.

Conflict of interest

We declare that there is no any conflict of interest.

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Authors and Affiliations

  1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

    Fatemeh Ahangari, Rasoul Salehi, Mansour Salehi & Hosein Khanahmad

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  1. Fatemeh Ahangari
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  2. Rasoul Salehi
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  3. Mansour Salehi
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  4. Hosein Khanahmad
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Correspondence to Rasoul Salehi.

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Ahangari, F., Salehi, R., Salehi, M. et al. A miRNA-binding site single nucleotide polymorphism in the 3′-UTR region of the NOD2 gene is associated with colorectal cancer. Med Oncol 31, 173 (2014). https://doi.org/10.1007/s12032-014-0173-7

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