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Investigation into the Effects of Boron on Liver Tissue Protein Carbonyl, MDA, and Glutathione Levels in Endotoxemia

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Abstract

Endotoxin has been known to cause the formation and damage of free radical. The importance of boron for human life is increasing each passing day, and its consuming fields are continuing to expand due to the advances in science and technology. Therefore, in our study, we intended to investigate into the effects of boron on liver tissue oxidative events. Eighteen male Wistar albino rats were randomly separated into three equal groups in the experiments; control group, boron + endotoxin group, and endotoxin group. Dissolved in distilled water, boric acid (100 mg/kg) was administered to boron + endotoxin group via gavage procedure for 28 days. Only distilled water was administered to control and endotoxin groups via gavage procedure for 28 days. Then 4 mg/kg endotoxin (LPS; Escherichia coli 0111:B4) was intraperitoneally (ip) administered to boron + endotoxin and endotoxin groups on the 28th day. Sterile saline was injected into control group on the 28th day (ip). Malondialdehyde (MDA), which is the end product of lipid peroxidation in liver tissues, protein carbonyl compounds (PC), which are protein oxidization markers, and glutathione (GSH) levels were measured spectrophotometrically. The results were compared with Mann–Whitney U test. When boron + endotoxin group is compared with endotoxin group, PC levels of endotoxin group showed a significant increase. When GSH levels are compared, GSH level in boron + endotoxin group decreased according to endotoxin group. Variations among all groups in MDA levels were found to be statistically insignificant. We are of the opinion that endotoxin affects the proteins by forming free radicals, and boron may also cause the structural and/or functional changes in proteins in order to protect proteins from oxidization.

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References

  1. Yeğenağa I (2006) Sepsis ve akut böbrek yetmezliğinde hemodinamik ve patofizyolojik değişiklikler. Türk Nefroloji Diyaliz ve Transplantasyon Dergisi 15(1):25–34

    Google Scholar 

  2. Armstrong TA, Spears JW (2003) Effect of boron supplementation of pig diets on the production of tumor necrosis factor-α and interferon-γ 1,2. J Anim Sci 81(10):2552–2561

    CAS  PubMed  Google Scholar 

  3. Er A, Dik B, Çetin G, Altan F, Üney K, Elmas M, Yazar E (2013) Etanersept—endotoksemi tedavisinde kullanılabilir mi? Kafkas Univ Vet Fak Derg 19(1):133–139. doi:10.9775/kvfd.2012.7357

    Google Scholar 

  4. Çelebioglu B, Özer E (2004) Kardiyopulmoner by-pass ve sistemik inflamatuvar yanıt. Hacettepe Tıp Dergisi 35:18–26

    Google Scholar 

  5. Scorei RI, Ciofrangeanu C, Ion R, Cimpean A, Galateanu B, Mitran V, Iordachescu D (2010) In vitro effects of calcium fructoborate upon production of inflammatory mediators by LPS-stimulated raw 264.7 macrophages. Biol Trace Elem Res 135(1–3):334–344. doi:10.1007/s12011-009-8488-5

    Article  CAS  PubMed  Google Scholar 

  6. Pleiner J, Heere-Ress E, Langenberger H (2002) Adrenoceptor hyporeactivity is responsible for Escherichia coli endotoxin-induced acute vascular dysfunction in humans. Arterioscler Thromb Vasc Biol 22(1):95–100

    Article  CAS  PubMed  Google Scholar 

  7. Nataro JP, Kaper JB (1998) Diarrheagenic Escherichia coli. Clin Microbiol Rev 11(1):142–201

    PubMed Central  CAS  PubMed  Google Scholar 

  8. Halliwell B, Gutteridge JM (2007) Free radicals, other reactive species and disease. In: Halliwell B, Gutteridge JM (eds) Free radicals in biology and medicine, 4th edn. Oxford University Press, New York, pp 617–783

    Google Scholar 

  9. Coşkun Ş, Peker EGG, Balabanlı B, Ahıska S, Acartürk F (2011) Effect of transforming growth factor beta 1 on nitric oxide production and lipid peroxidation in oral mucosal wound healing. Med Chem Res 20(1):23–28. doi:10.1007/s00044-009-9276-7

    Article  Google Scholar 

  10. Balabanlı B, Turkozkan N, Akmansu M, Polat M (2008) Exacerbative role of vitamin A on radiation damage in vivo. 17(1):12–18. doi:10.1007/s00044-008-9088-1

  11. Coşkun Ş, Güleç EG, Balabanlı B, Acartürk F (2007) Effects of epidermal growth factor on lipid peroxidation and nitric oxide levels in oral mucosal ulcer healing: a time-course study. Surg Today 37(7):570–574. doi:10.1007/s00595-006-3455-7

    Article  PubMed  Google Scholar 

  12. Çınar E, Avcı E, Peker EGG, Cevher CŞ (2011) Dönüştürücü büyüme faktörü betanın tükürük bezi oksidan olaylarına zaman bağımlı etkisi. Türk Biyokimya Dergisi 36(1):15–20

    Google Scholar 

  13. Ozan G, Turkozkan N, Bircan FS, Balabanlı B (2012) Effect of taurine on brain 8-hydroxydeoxyguanosine and 3-nitrotyrosine levels in endotoxemia. Inflammation 35(2):665–670. doi:10.1007/s10753-011-9359-8

    Article  CAS  PubMed  Google Scholar 

  14. Özgün SG, Eskiocak S, Süt N (2010) Streptozotosin ile diyabet geliştirilmiş sıçanlarda L-karnitinin protein oksidasyonu üzerine etkisi. Türk Biyokimya Dergisi 35(3):183–189

    Google Scholar 

  15. Bircan FS, Balabanlı B, Turkozkan N, Ozan G (2011) Effects of taurine on nitric oxide and 3-nitrotyrosine levels in spleen during endotoxemia. Neurochem Res 36(11):1978–1983. doi:10.1007/s11064-011-0521-3

    Article  CAS  PubMed  Google Scholar 

  16. Almroth BC, Sturve J, Berglund A, Forlin L (2005) Oxidative damage in eelpout (Zoarcesviviparus), measured as protein carbonyls and TBARS, as biomarkers. Aquat Toxicol 73(2):171–180. doi:10.1016/j.aquatox.2005.03.007

    Article  CAS  PubMed  Google Scholar 

  17. Çakatay U, Telci A, Yılmaz IA, Akçay T, Sivas A (2000) The effect of aging on plasma oxidative protein damage. Cerrahpaşa J Med 31(4):220–223

    Google Scholar 

  18. El-Gendy KS, Aly NM, Mahmoud FH, Kenawy A, El-Sebae AKH (2010) The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid. Food Chem Toxicol 48(1):215–221. doi:10.1016/j.fct.2009.10.003

    Article  CAS  PubMed  Google Scholar 

  19. Sogut I, Oglakcı A, Kartkaya K (2015) Effect of boric acid on oxidative stress in rats with fetal alcohol syndrome. Exp Ther Med 9(3):1023–1027. doi:10.3892/etm.2014.2164

    PubMed Central  CAS  PubMed  Google Scholar 

  20. Yılmaz A (2002) Her derde deva hazinemiz bor. TUBİTAK-Bilim ve Teknik Dergisi 414:38–41

    Google Scholar 

  21. Mohora M, Boghianu L, Muscurel C, Duta C, Dumitrache C (2002) Effects of boric acid on redox status in the rat liver. Rom J Biophys 12(3–4):77–82

    CAS  Google Scholar 

  22. Karadağ GM, Türközü D (2014) Diyetle bor alımının sağlık ile etkileşimi: güncel bakış. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi 3(2):771–779

    Google Scholar 

  23. Cao J, Jiang L, Zhang X, Yao X, Geng C, Xue X, Zhong L (2008) Boric acid inhibits LPS-induced TNF-alpha formation through a thiol-dependent mechanism in THP-1 cells. J Trace Elem Med Biol 22(3):189–195. doi:10.1016/j.jtemb.2008.03.005

    Article  CAS  PubMed  Google Scholar 

  24. Reznick AZ, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357–363. doi:10.1016/S0076-6879(94)33041-7

    Article  CAS  PubMed  Google Scholar 

  25. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478

    Article  CAS  PubMed  Google Scholar 

  26. Aykaç G, Uysal M, Yalçin AS, Koçak-Toker N, Sivas A, Öz H (1985) The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicology 36(1):71–76. doi:10.1016/0300-483x(85)90008-3

    Article  PubMed  Google Scholar 

  27. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310. doi:10.1016/S0076-6879(78)52032-6

    Article  CAS  PubMed  Google Scholar 

  28. Barton CC, Barton EX, Ganey PE, Kunkel SL, Roth RA (2001) Bacterial lipopolysaccharide enhances aflatoxin b1 hepatotoxicity in rats by a mechanism that depends on tumor necrosis factor alpha. Hepatology 33(1):66–73. doi:10.1053/jhep.2001.20643

    Article  CAS  PubMed  Google Scholar 

  29. Sato K, Kawana M, Nonomura N, Nakano Y (1999) Course of IL-1 beta, IL-6, IL-8 and TNF-alpha in the middle ear fluid of the guinea pig otitis media model induced by nonviable haemophilus influenzae. Ann Otol Rhinol Laryngol 108(6):559–563

    Article  CAS  PubMed  Google Scholar 

  30. Erdamar H, Turkozkan N, Balabanlı B, Ozan G, Bircan FS (2007) The relationship between taurine and 3-nitrotyrosine level of hepatocytes in experimental endotoxemia. Neurochem Res 32(11):1965–1968. doi:10.1007/s11064-007-9395-9

    Article  CAS  PubMed  Google Scholar 

  31. Bozkurt B, Koç M, Coşkun F, Cengiz O, Bilgin A, Demirpençe E, Kılınç K, Aksoy F (1998) Ratlarda oluşturulan endotoksemide glutatyon eksikliğinin karaciğer histopatoloji ve fonksiyonlarına etkisi. Türkiye Klinikleri Tıp Bilimleri Dergisi 18(6):388–397

    Google Scholar 

  32. Kaymak C, Başar H, Sardas S (2011) Reactive oxygen species generation in sepsis. FABAD J Pharm Sci 36(1):41

    Google Scholar 

  33. Nielsen F (2000) The emergence of boron as nutritionally important throughout the life cycle. Nutrition 16(7):512–514

    Article  CAS  PubMed  Google Scholar 

  34. Eckhert C (1998) Boron stimulates embryonic trout growth. J Nutr 128(12):2488–2493

    CAS  PubMed  Google Scholar 

  35. Hunt C, Herbel L, Nielsen F (1997) Metabolic responses of postmenopausal women to supplementation dietary boron and aluminum during usual and low magnesium intake: boron, calcium and magnesium absorption an retention and blood mineral concentration. Am J Clin Nutr 65(3):803–813

    CAS  PubMed  Google Scholar 

  36. İnce S, Kücükkurt I, Ciğerci IH, Fidan AF, Eryavuz A (2010) The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity and DNA damage in rats. J Trace Elem Med Biol 24(3):161–164. doi:10.1016/j.jtemb.2010.01.003

    Article  PubMed  Google Scholar 

  37. Türkez H, Geyikoğlu F, Tatar A, Keles MS, Kaplan I (2012) The effects of some boron compounds againts heavy metal toxicity in human blood. Exp Toxicol Pathol 64(1):93–101. doi:10.1016/j.etp.2010.06.011

    Article  PubMed  Google Scholar 

  38. Ardıç M, Sekmen AH, Tokur S, Özdemir F, Türkan I (2009) Antioxidant responses of chickpea plants subjected to boron toxicity. Plant Biol 11(3):328–338. doi:10.1111/j.1438-8677.2008.00132.x

    Article  PubMed  Google Scholar 

  39. Eraslan F, İnal A, Savasturk O, Günes A (2007) Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity. Sci Hortic 114(1):5–10

    Article  CAS  Google Scholar 

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Compliance with Ethical Standards

This paper is in compliance with ethical standards. There is no potential conflict of interest. Research involves animals. A total of 18 male rats (Wistar Albino), which were obtained from Gazi University, Animals Reproduction Laboratory and Experimental Research Centre (GUDAM), were used in the experiments. The protocol of study was approved by the ethical review committee of the institute. All other authors have read the manuscript and have agreed to submit it in its current form for consideration for publication in the journal.

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  1. Department of Biology, Science Faculty, Gazi University, Besevler, Ankara, Turkey

    Barbaros Balabanlı & Tuba Balaban

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  1. Barbaros Balabanlı
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  2. Tuba Balaban
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Correspondence to Barbaros Balabanlı.

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Balabanlı, B., Balaban, T. Investigation into the Effects of Boron on Liver Tissue Protein Carbonyl, MDA, and Glutathione Levels in Endotoxemia. Biol Trace Elem Res 167, 259–263 (2015). https://doi.org/10.1007/s12011-015-0301-z

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