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Manganese-Induced Effects on Testicular Trace Element Levels and Crucial Hormonal Parameters of Hyline Cocks

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Abstract

Manganese (Mn) is an essential element required for normal development and reproduction. However, little is known about the reproductive toxicity of Mn in birds. To investigate the Mn-induced toxicity on testicular trace element levels and crucial hormonal parameters on male reproduction in birds, 50-day-old male Hyline cocks were fed either a commercial diet or a Mn-supplemented diet. The changes in contents of copper (Cu), iron (Fe), zinc (Zn), and calcium (Ca) in testis were detected. Hormonal parameters were evaluated including the levels of testosterone (T), luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), triiodothyronine (T3), and thyroxine (T4) in the serum. The mRNA levels of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) were determined in this study. The results showed that Mn was accumulated in testis, and the content of Cu, Fe, Zn, and Ca decreased. Exposure to Mn significantly lowered the content of T, LH, FSH, and the mRNA expression levels of LHR and FSHR. Levels of T3 and T4 appeared with a decreased tendency, and TSH presented no obvious regularity. It indicated that Mn exposure resulted in the disbalance of testicular trace elements and influenced hormone levels in the molecular level, which may be possible underlying reproductive toxicity mechanism induced by Mn.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (30871902), Youth Science Foundation of Heilongjiang Province (QC2010026), and Scientific Research Foundation for Doctor of Harbin University of Commerce (12DL014).

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Authors and Affiliations

  1. Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin, 150076, People’s Republic of China

    Xiao-fei Liu & Ning Liu

  2. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Li-ming Zhang, Ziwei Zhang, Shi-wen Xu & Hong-jin Lin

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  1. Xiao-fei Liu
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  2. Li-ming Zhang
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  3. Ziwei Zhang
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  4. Ning Liu
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Correspondence to Shi-wen Xu or Hong-jin Lin.

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X. Liu and L. Zhang contributed equally to this work.

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Liu, Xf., Zhang, Lm., Zhang, Z. et al. Manganese-Induced Effects on Testicular Trace Element Levels and Crucial Hormonal Parameters of Hyline Cocks. Biol Trace Elem Res 151, 217–224 (2013). https://doi.org/10.1007/s12011-012-9549-8

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  • DOI: https://doi.org/10.1007/s12011-012-9549-8

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