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Properties of Polyhydroxyalkanoate Granules and Bioemulsifiers from Pseudomonas sp. and Burkholderia sp. Isolates Growing on Glucose

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Abstract

A Burkholderia and Pseudomonas species designated as AB4 and AS1, respectively, were isolated from soil containing decomposing straw or sugar cane bagasse collected from Brazil. This study sought to evaluate the capacities of culture media, cell-free medium, and crude lysate preparations (containing PHB inclusion bodies) from bacterial cell cultures to stabilize emulsions with several hydrophobic compounds. Four conditions showed good production of bioemulsifiers (E24 ≥ 50 %), headed by substantially cell-free media from bacterial cell cultures in which bacterial isolates from Burkholderia sp. strain AB4 and Pseudomonas sp. strain AS1 were grown. Our results revealed that the both isolates (AB4 and AS1 strains) exhibited high emulsification indices (indicating usefulness in bioremediation) and good stabilities.

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Acknowledgments

The authors acknowledge the Program of Agricultural Microbiology FCAV/UNESP Campus Jaboticabal and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for providing a PROAP doctoral scholarship, Post-doctoral National Program (PNPD/CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support.

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Authors and Affiliations

  1. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP–Univ Estadual Paulista, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, SP, Brazil

    Laís Postai Sacco, Tereza Cristina Luque Castellane, Erica Mendes Lopes, Eliana Gertrudes de Macedo Lemos & Lúcia Maria Carareto Alves

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  1. Laís Postai Sacco
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  2. Tereza Cristina Luque Castellane
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  3. Erica Mendes Lopes
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Correspondence to Tereza Cristina Luque Castellane or Lúcia Maria Carareto Alves.

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Sacco, L.P., Castellane, T.C.L., Lopes, E.M. et al. Properties of Polyhydroxyalkanoate Granules and Bioemulsifiers from Pseudomonas sp. and Burkholderia sp. Isolates Growing on Glucose. Appl Biochem Biotechnol 178, 990–1001 (2016). https://doi.org/10.1007/s12010-015-1923-5

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