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Characterization of a novel bioemulsifier from Pseudomonas stutzeri

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Abstract

This study describes a novel and efficient alasan-like bioemulsifier produced by Pseudomonas stutzeri NJtech 11-1, which was isolated from the Shengli Oilfield. The strain was found to produce a new and interesting emulsion stabilizer. The crude bioemulsifier showed super stability with 50% salinity and broad pH 3–10. The emulsion index (EI24) was increased to 100% after heating from 45 to 95 °C and the emulsion could be stable for at least 30 days. The yield of Ps-bioemulsifier (pure bioemulsifier) was 0.68 ± 0.05 mg mL−1. The Ps-bioemulsifier was composed of carbohydrates (80 ± 2.6%) and proteins (9.5 ± 0.5%). A low concentration (0.2 mg mL−1) of the Ps-bioemulsifier was obtained maximum emulsifying activity at pH 7.1 and its emulsifying activity strengthened by suitable salinity. Furthermore, Ps-bioemulsifier could also emulsify cyclohexane, hexadecane, kerosene, xylene hydrocarbons efficiently. Therefore, the Ps-bioemulsifier showed emulsifying characteristics which make it a good candidate for potential applications in bioremediation and microbial enhanced oil recovery.

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Acknowledgements

This work is supported by National Science Foundation of China (No. 21576133) and the Program for Innovative Research Team in University of Jiangsu Province.

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Authors and Affiliations

  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 5 Xinmofan Road, Nanjing, 210009, China

    Yanqiu Fan, Weiyi Tao & Shuang Li

  2. School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China

    He Huang

  3. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, China

    He Huang & Shuang Li

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  1. Yanqiu Fan
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  2. Weiyi Tao
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  4. Shuang Li
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Correspondence to Shuang Li.

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Fan, Y., Tao, W., Huang, H. et al. Characterization of a novel bioemulsifier from Pseudomonas stutzeri . World J Microbiol Biotechnol 33, 161 (2017). https://doi.org/10.1007/s11274-017-2326-2

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