Abstract
Studies were conducted on a mixture of pollutants commonly found in coke oven wastewater (CWW) to evaluate the biodegradation of various pollutants under anaerobic, aerobic, and anoxic conditions. The removal of the pollutants was monitored during individual bioreactor operation and using a combination of bioreactors operating in anaerobic–aerobic–anoxic sequence. While studying the performance of individual reactors, it was observed that cyanide removal (83.3 %) was predominant in the aerobic bioreactor, while much of the chemical oxygen demand (COD) (69 %) was consumed in the anoxic bioreactor. With the addition of cyanide, the COD removal efficiency was affected in all the bioreactors, and several intermediates were detected. While treating synthetic CWW using the combined bioreactor system, the overall COD removal efficiency was 86.79 % at an OLR of 2.4 g COD/L/day and an HRT of 96 h. The removal efficiency of 3,5-xylenol and cyanide, with inlet concentration of 150 and 10 mg/L, was found to be 91.8 and 93.6 % respectively. It was found that the impact of xylenol on the performance of the bioreactors was less than cyanide toxicity. Molecular analysis using T-RFLP revealed the dominance of strictly aerobic, mesophilic proteobacterium, Bosea minatitlanensis, in the aerobic bioreactor. The anoxic bioreactor was dominant with Rhodococcus pyridinivorans, known for its remarkable aromatic decomposing activity, while an unclassified Myxococcales bacterium was identified as the predominant bacterial species in the anaerobic bioreactor.
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Wang, W., Ma, W., Han, H., Li, H., & Yuan, M. (2011). Bioresource Technology, 102, 2441–2447.
Lai, P., Zhao, H., Zeng, M., & Ni, J. (2009). Journal of Hazardous Material, 162, 1423–1429.
Marañón, E., Vázquez, I., & Rodríguez, J. (2008). Water Air Soil Pollution, 192, 155–164.
Vazquez, I., Rodrıguez, J., Maraon, E., Castrillon, L., & Fernandez, Y. (2006). Journal of Hazardous Matererials, 137, 1681–1688.
Wang, W., & Han, H. (2012). Bioresource Technology, 103, 95–100.
Park, D., Mo, Y., Sung, D., & Moon, J. (2008). Chemical Engineering Journal, 143, 141–146.
Kumar, M. S., Vaidya, A. N., Shivaraman, N., & Bal, A. S. (2000). Environmental Engineering Science, 17, 221–226.
Lai, P., Zhao, H., Ye, Z., & Ni, J. R. (2008). Process Biochemistry, 43, 229–237.
Bajaj, M., Gallert, C., & Winter, J. (2009). Biochemical Engineering Journal, 46, 205–209.
Dash, R. R., Gaur, A., & Balomajumder, C. (2009). Journal of Hazardous Materials, 163, 1–11.
Zaher, U., Moussa, M. S., Widyatmika, I. N., Steen, P., Gijzen, H. J., & Vanrolleghem, P. (2006). Water Science and Technology, 54, 129–137.
Li, Y., Gu, G., Zhao, J., & Yu, H. (2001). Process Biochemistry, 37, 81–86.
Bai, Y., Sun, Q., Zhao, C., & Wen, D. (2009). Environmetnal Biotechnology, 82, 963–973.
Saravanan, P., Pakshirajan, K., & Saha, P. (2008). Journal of Environmental Sciences, 20, 1508–1513.
Fedorak, P. M., & Hrudey, S. E. (1989). Water Science and Technology, 21, 67–76.
Shieh, W. K., & Richards, D. J. (1988). Journal of Environmental Engineering, 114, 639–654.
Amor, L., Eiroa, M., Kennes, C., & Veiga, M. C. Ã. (2005). Water Research, 39, 2915–2920.
Flores, E. R., Iniestra-gonza, M., Field, J. A., Olguı, P., & Puig-grajales, L. (2003). Jouranl of Environmental Engineering, 129, 999–1006.
Fetzner, S. (1998). Applied Microbiology and Biotechnology, 49, 237–250.
Chakraborty, S., & Veeramani, H. (2006). Process Biochemistry, 41, 96–105.
Vazquez, I., Rodrıguez, J., Maranon, E., Castrillnon, E., & Fernandez, D. (2006). Journal of Hazardous Materials, 137, 1773–1780.
El-Deeb, B. A. (2006). World Journal of Microbiology and Biotechnology, 16, 1068–1077.
Mao, Y., Zhang, X., & Xia, X. (2010). Journal of Industrial Microbiology, 37, 927–934.
Felföldi, T., Székely, A. J., Gorál, R., Barkács, K., Scheirich, G., & András, J. (2010). Bioresource Technology, 101, 3406–3414.
Mo, Y., Sung, D., Park, C., Park, D., & Moon, J. (2010). Water Research, 45, 1267–1279.
Saravanan, P., Pakshirajan, K., & Saha, P. (2009). Journal of Hazardous Materials, 162, 476–48.
Khoury, N., Dott, W., & Peter, K. (1992). Applied Microbiology and Biotechnology, 37, 524–528.
APHA (2005). Standard methods for examination of water and wastewater examination, 21 st Ed. Washington DC.
Zhu, S., Liu, D., Fan, L., & Ni, J. (2008). Journal of Hazardous Materials, 160, 289–294.
Aislabie, J., Bej, A. K., Hurst, H., Rothenburger, S., & Atlas, R. M. (1990). Applied and Environmental Microbiology, 56, 345–351.
Lowry, O. H., Rosebrough, N. J., Farr, L., & Randall, R. J. (1951). Journal of Biological Chemistry, 193, 265–275.
Sharma, N. K., Philip, L., & Murty, B. S. (2012). Bioresource Technology, 121, 263–273.
Farooqi, I. H., Basheer, F., & Ahmad, T. (2008). Global NEST Journal, 10, 39–46.
Licht, D., Johansen, S. S., Arvin, E., & Ahring, B. K. (1997). Applied Microbiology and Biotechnology, 47, 167–172.
Ramakrishnan, A., & Gupta, S. K. (2006). Journal of Hazardous Materials, 137, 1488–1495.
Yongmei, L. I., Wenshu, L. I., & Guowei, G. U. (2007). Frontiers of Environmental Science Engineering China, 1(4), 493–497.
Fedorak, P. M., & Hrudey, S. E. (1987). Water Science & Technology, 19, 219–228.
Acuña-Argüelles, M. E., Olguin-Lora, P., & Razo-Flores, E. (2003). Biotechnology Letters, 25(7), 559–564.
Fallon, R. D. (1992). Applied and Environmental Microbiology, 58(9), 3163–3164.
Naveen, D., Majumder, C. B., Mondal, P., & Shubha, D. (2011). Research Journal of Chemical Sciences, 1(7), 15–21.
Saravanan, P., Pakshirajan, K., & Saha, P. (2008). Bioresource Technology, 99, 8553–8558.
Yao, H., Ren, Y., Wei, C., & Yue, S. (2011). Water SA, 37(1), 15–20.
Jianlong, W., Xiangchun, Q., Liping, H., Yi, Q., & Hegemann, W. (2002). Water Research, 36, 2288–2296.
Ozturk, A., & Abdullah, M. I. (2006). Science of the Total Environment, 358, 137–142.
Ravikumar, S., Gokulakrishnan, R., & Parimala, P. S. (2011). International journal of current Research, 3(7), 96–100.
Banerjee, A., & Ghoshal, A. K. (2010). Bioresource Technology, 101(14), 5501–5507.
Semple, K. T., & Cain, R. B. (1997). FEMS Microbiology Letters, 152, 133–139.
Gijzen, H. J., Bernal, E., & Ferrer, H. (2000). Water Research, 34, 2447–2454.
Haghighi-Podeh, M. R., & Siyahati, A. G. (2000). Water Science & Technology, 42(3), 125–130.
Kang, M. H., & Park, J. M. (1997). Journal of Chemical Technology and Biotechnology, 69, 226–230.
Neufeld, R., Greefield, J., & Rieder, B. (1986). Water Research, 20(5), 633–642.
González-blanco, G., Beristain-cardoso, R., Cuervo-lópez, F., Cervantes, F. J., & Gómez, J. (2012). Bioresource Technology, 103, 48–55.
O’Connor, O. W., & Young, D. L. (1996). Environmental Science and Technology, 30(5), 1419–1428.
Max, M. H., Rivera, M. D., Bossert, I. D., Rogers, J. E., & Young, L. Y. (1990). Microbial Ecology, 20, 141–150.
Thomas, S., Sarfaraz, S., Mishra, L. C., & Iyengar, L. (2002). World Journal of Microbiology and Biotechnology, 18, 57–63.
Deka, B. J., Sahariah, B. P., & Chakraborty, S. (2008). Journal of Environmental Research and Development, 3(2), 442–448.
Zhang, M., Tay, J. H., Qian, Y., & Gu, X. S. (1997). Journal of Environmental Engineering, 123(9), 876–883.
Ouattara, A. S., Assih, E. A., Thierry, S., Cayol, J. L., Labat, M., Monroy, O., & Macarie, H. (2003). International Journal of Systematic and Evolutionary Microbiology, 53, 1247–1251.
Acosta-González, A., Rosselló-Móra, R., & Marqués, S. (2013). Environmental Microbiology, 15(1), 77–92.
Kundu, D., Hazra, C., Dandi, N., & Chaudhari, A. (2013). Biodegradation, 24(6), 775–793.
Yoon, J. H., Kang, S. S., Cho, Y. G., Lee, S. T., Kho, Y. H., Kim, C. J., & Park, Y. H. (2000). International Journal of Systematic and Evolutionary Microbiology, 50, 2173–2180.
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Sharma, N.K., Philip, L. Treatment of Phenolics, Aromatic Hydrocarbons, and Cyanide-Bearing Wastewater in Individual and Combined Anaerobic, Aerobic, and Anoxic Bioreactors. Appl Biochem Biotechnol 175, 300–322 (2015). https://doi.org/10.1007/s12010-014-1262-y
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DOI: https://doi.org/10.1007/s12010-014-1262-y