Skip to main content
SpringerLink
Log in

Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced β-Glucosidase from a Marine Streptomycete

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The gene BglNH encoding a β-glucosidase was cloned from a marine streptomycete. Sequence analysis revealed that BglNH encoded a 456-aa peptide with a calculated mass of 51 kDa. The deduced amino acid sequence of BglNH showed the highest identities of 61 % with known β-glucosidases and contained a catalytic domain which belonged to the glycoside hydrolase family 1. The gene BglNH was expressed in Escherichia coli and the recombinant enzyme (r-BglNH) was purified. The optimum pH and temperature of r-BglNH were pH 6.0 and 45 °C, respectively. The r-BglNH displayed the typical salt-tolerant and glucose-enhanced characteristics. Its activity was remarkably enhanced in the presence of 0.5 M NaCl (rose more than 1.6-fold) and 0.1 M glucose (rose more than 1.4-fold). Moreover, r-BglNH displayed good pH stability and metal tolerance. It remained stable after incubating with buffers from pH 4.0 to 10.0, and most metal ions had no significant inhibition on its activity. These properties indicate that r-BglNH is an ideal candidate for further research and industrial applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Bhatia, Y., Mishra, S., & Bisaria, V. (2002). Critical Reviews in Biotechnology, 22, 375–407.

    Article  CAS  Google Scholar 

  2. Bradford, M. M. (1976). Analytical Biochemistry, 72, 248–254.

    Article  CAS  Google Scholar 

  3. Bronnenmeier, K., & Staudenbauer, W. L. (1988). Applied Microbiology and Biotechnology, 28, 380–386.

    Article  CAS  Google Scholar 

  4. Debashish, G., Malay, S., Barindra, S. & Joydeep, M. (2005). Marine Biotechnology I, 189–218.

  5. Fan, H. X., Miao, L. L., Liu, Y., Liu, H. C. & Liu, Z. P. (2011). Enzyme and microbial technology.

  6. Gao, Z., Ruan, L., Chen, X., Zhang, Y., & Xu, X. (2010). Applied Microbiology and Biotechnology, 87, 1373–1382.

    Article  CAS  Google Scholar 

  7. Gilbert, H. J., Stålbrand, H., & Brumer, H. (2008). Current Opinion in Plant Biology, 11, 338–348.

    Article  CAS  Google Scholar 

  8. Hayashi, S., Sako, S., Yokoi, H., Takasaki, Y., & Imada, K. (1999). Journal of Industrial Microbiology & Biotechnology, 22, 160–163.

    Article  CAS  Google Scholar 

  9. Hirasawa, K., Uchimura, K., Kashiwa, M., Grant, W. D., Ito, S., Kobayashi, T., et al. (2006). Antonie Van Leeuwenhoek, 89, 211–219.

    Article  CAS  Google Scholar 

  10. Hong, M. R., Kim, Y. S., Park, C. S., Lee, J. K., & Oh, D. K. (2009). Journal of Bioscience and Bioengineering, 108, 36–40.

    Article  CAS  Google Scholar 

  11. Karnchanatat, A., Petsom, A., Sangvanich, P., Piaphukiew, J., Whalley, A. J. S., Reynolds, C. D., et al. (2007). FEMS Microbiology Letters, 270, 162–170.

    Article  CAS  Google Scholar 

  12. Ketudat Cairns, J. R., & Esen, A. (2010). Cellular and Molecular Life Sciences, 67, 3389–3405.

    Article  CAS  Google Scholar 

  13. Klyosov, A. A. (1990). Biochemistry, 29, 10577–10585.

    Article  CAS  Google Scholar 

  14. Kusama, S., Kusakabe, I., & Murakami, K. (1986). Agricultural and Biological Chemistry, 50, 2891–2898.

    Article  CAS  Google Scholar 

  15. Lineweaver, H., & Burk, D. (1934). Journal of the American Chemical Society, 56, 658–666.

    Article  CAS  Google Scholar 

  16. Mamma, D., Hatzinikolaou, D. G., & Christakopoulos, P. (2004). Journal of Molecular Catalysis B: Enzymatic, 27, 183–190.

    Article  CAS  Google Scholar 

  17. Mao, X., Hong, Y., Shao, Z., Zhao, Y., & Liu, Z. (2010). Applied Biochemistry and Biotechnology, 162, 2136–2148.

    Article  CAS  Google Scholar 

  18. Mccarthy, A. (1987). FEMS Microbiology Letters, 46, 145–163.

    Article  CAS  Google Scholar 

  19. Mccarthy, A. J., & Williams, S. T. (1992). Gene, 115, 189–192.

    Article  CAS  Google Scholar 

  20. Moldoveanu, N., & Kluepfel, D. (1983). Applied and Environmental Microbiology, 46, 17–21.

    CAS  Google Scholar 

  21. Odoux, E., Escoute, J., Verdeil, J. L., & Brillouet, J. M. (2003). Annals of Botany, 92, 437–444.

    Article  CAS  Google Scholar 

  22. Pérez-Pons, J. A., Rebordosa, X., & Querol, E. (1995). Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1251, 145–153.

    Article  Google Scholar 

  23. Perez-Pons, J. A., Cayetano, A., Rebordosa, X., Lloberas, J., Guasch, A., & Querol, E. (1994). European Journal of Biochemistry, 223, 557–565.

    Article  CAS  Google Scholar 

  24. Shewale, J. (1982). The International Journal of Biochemistry, 14, 435.

    Article  CAS  Google Scholar 

  25. Spear, L., Gallagher, J., Mchale, L., & Mchale, A. (1993). Biotechnology Letters, 15, 1265–1268.

    Article  CAS  Google Scholar 

  26. Sveinbjörnsson, J., Murphy, M., & Udén, P. (2007). Animal Feed Science and Technology, 132, 171–185.

    Article  Google Scholar 

  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., & Higgins, D. G. (1997). Nucleic Acids Research, 25, 4876–4882.

    Article  CAS  Google Scholar 

  28. Voorhorst, W., Eggen, R., Luesink, E. J., & De Vos, W. (1995). Journal of Bacteriology, 177, 7105–7111.

    CAS  Google Scholar 

  29. Wood, T. M., & Mccrae, S. I. (1982). Journal of General Microbiology, 128, 2973–2982.

    CAS  Google Scholar 

  30. Wright, R. M., Yablonsky, M. D., Shalita, Z. P., Goyal, A., & Eveleigh, D. (1992). Applied and Environmental Microbiology, 58, 3455–3465.

    CAS  Google Scholar 

  31. Zemin, F., Fang, W., Liu, J., Hong, Y., Peng, H., Zhang, X., et al. (2010). Journal of Microbiology and Biotechnology, 20, 1351–1358.

    Article  Google Scholar 

  32. Zhou, J., Zhang, R., Shi, P., Huang, H., Meng, K., Yuan, T., et al. (2011). Applied microbiology and biotechnology, 1–11.

Download references

Acknowledgments

This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104).

Author information

Authors and Affiliations

  1. Key Laboratory of Marine Bio-resources Sustainable Utilization CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, People’s Republic of China

    Zhimao Mai, Jian Yang, Xinpeng Tian, Jie Li & Si Zhang

  2. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Zhimao Mai, Jian Yang & Si Zhang

Authors
  1. Zhimao Mai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jian Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xinpeng Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jie Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Si Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Si Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mai, Z., Yang, J., Tian, X. et al. Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced β-Glucosidase from a Marine Streptomycete . Appl Biochem Biotechnol 169, 1512–1522 (2013). https://doi.org/10.1007/s12010-012-0080-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-0080-3

Keywords

Search

Navigation