Abstract
Enzymes such as α-amylase are extensively used to retard the staling process. Enzymes are acting both during fermentation and during baking. The objective of this work was to determine the relative action of α-amylase during fermentation and during baking. The impact of the baking conditions (time, temperature) was also considered. To attain this aim, a degassed bread crumb was baked in a miniaturized system using two programs of baking: heating rates 10.27 and 6.88 °C/min corresponding to 180 and 220 °C baking temperatures, respectively. Mechanical and thermodynamic properties of the degassed crumb were assessed during aging of bread by determining the Young’s modulus E, the amount of freezable water, and the melting enthalpy of retrograded amylopectin. A first-order kinetic model was used to determine the different parameters of staling kinetics. Results showed that the hardening of crumb increased during storage. The kinetics were faster for samples baked with fast heating rate than for those baked with slow heating rates. The use of enzymes decreased the Young’s modulus but did not have any effect on the staling rate. Calorimetric analysis of the starch retrogradation showed a reduction of the amount of freezable water during storage with an increase of retrograded amylopectin. A comparison between mechanical properties of conventional crumb and of the degassed dough confirmed that experimental data fitted correctly the Gibson and Ashby’s model.
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References
Angioloni, A., & Collar, C. (2009). Bread crumb quality assessment: a plural physical approach. European Food Research and Technology, 229, 21–30.
AOAC (1995). Official methods of analysis of AOAC International: Methods 952.10 and 926.05. The Association (16th edn), 1–4.
Baik, M. Y., & Chinachoti, P. (2000). Moisture redistribution and phase transitions during bread staling. Cereal Chemistry, 77(4), 484–488.
Baik, M. Y., & Chinachoti, P. (2001). Effects of glycerol and moisture gradient on thermomechanical properties of white bread. Journal of Agriculture and Food Chemistry, 49(8), 4031–4038.
Bechtel, W. G., Meisner, D. F., & Bradley, W. B. (1953). Effect of the crust on the staling of bread. Food Technology, 30, 160–168.
Corford, S. J., Axford, D. W. E., & Elton, G. A. H. (1964). The elastic modulus of bread crumb in linear compression in relation to staling. Cereal Chemistry, 41, 216–229.
Eliasson, A. C. (1985). Retrogradation of starch as measured by differential scanning calorimetry. In R. D. Hill & L. Munck (Eds.), New approaches to researches on cereal carbohydrates. Amsterdam: Elsevier Science.
Eliasson, A. C. (2003). Utilization of thermal properties for understanding baking processes: V staling of bread. In K. Gonul & J. B. Kanneth (Eds.), Characterization of cereals and flours: properties analysis and applications. New York: Basel.
Fessas, D., & Schiraldi, A. (2001). Water properties in wheat flour dough I: classical thermogravimetry approach. Food Chemistry, 72, 237–244.
Gambaro, A., Varela, P., Gimenez, A., Aldrovandi, A., Fiszman, S. M., & Hough, G. (2002). Textural quality of white pan bread by sensory and instrumental measurements. Journal of Texture Studies, 33, 401–413.
Gibson, L. J., & Ashby, M. F. (1997). Cellular solids—structure and properties. Cambridge: Cambridge University Press.
Giovanelli, G., Peri, C., & Borri, V. (1997). Effects of baking temperature on crumb staling kinetics. Cereal Chemistry, 74, 710–714.
Gomez, M., Oliete, B., Pando, V., Ronda, F., & Caballero, P. A. (2008). Effect of fermentation conditions on bread staling kinetics. European Food Research and Technology, 226, 1379–1987.
Gray, J. A., & Bemiller, J. N. (2003). Bread staling: molecular basis and control. Comprehensive Reviews in Food Science and Food Safety, 2, 1–21.
Guessasma, S., Babin, P., Della_ Valle, G., & Dendievel, R. (2008). Relating cellular structure of open solid food foams to their Young’s modulus: finite element calculation. International Journal of Solids and Structures, 45, 2881–2896.
Hallberg, L., & Chinachoti, P. (2002). A fresh perspective on staling: the significance of starch recrystallisation on the firming of bread. Journal of Food Science, 67, 1092–1096.
Haros, M., Rosell, C. M., & Benedito, C. (2002). Effect of different carbohydrases on fresh bread texture and bread staling. European Food Research and Technology, 215, 425–430.
Hug-Iten, S., Handschin, S., Conde-Petit, B., & Escher, F. (1999). Changes in starch microstructure on baking and staling of wheat bread. Lebensmittel-Wissenschaft und-Technologie, 32, 255–260.
Kim, S. K., & D’Appolonia, B. L. (1977). Bread staling studies I: effect of protein content on staling rate and bread crumb pasting properties. Cereal Chemistry, 54, 207.
Le Bail, A., Boumali, K., Jury, V., Ben-Aissa, F., & Zuniga, R. (2009). Impact of baking kinetics on staling rate and mechanical properties of bread crumb and degassed bread crumb. Journal of Cereal Science, 50, 235–240.
Patel, B. K., Waniska, R. D., & Seetharaman, K. (2005). Impact of different baking processes on bread firmness and starch properties in breadcrumb. Journal of Cereal Sciences, 42, 173–184.
Poinot, P., Arvisenet, G., Grua-Priol, J., Colas, D., Fillonneau, C., Le-Bail, A., et al. (2008). Influence of formulation and process on the aromatic profile and physical characteristics of bread. Journal of Cereal Sciences, 48, 686–697.
Ribotta, P. D., & Le Bail, A. (2007). Thermo-physical assessment of bread during staling. Lebensmittel-Wissenschaft und Technologie, 40, 879–884.
Ribotta, P. D., Cuffini, S., & Leon, A. E. (2004). The staling of bread: an X-ray diffraction study. European Food Research and Technology, 218, 219–223.
Schultz, A. S., Schoonover, F. D., Fisher, R. A., & Jacker, S. S. (1952). Retardation of crumb starch staling in commercial bread by bacterial alpha-amylase. Cereal Chemistry, 29, 200.
Vodovotz, Y., Hallberg, L., & Chinachoti, P. (1996). Effect of aging and drying on thermomechanical properties of white bread as characterized by dynamical mechanical analysis (DMA) and differential scanning calorimetry (DSC). Cereal Chemistry, 73(2), 264–270.
Willhoft, E. M. A. (1971). Bread staling I: experimental study. Journal of the Science of Food and Agriculture, 22, 176–180.
Acknowledgments
This work was supported by the government of Tunisia (Ph.D. grant of E. Besbes) and by the Scientific Council of ONIRIS (Ph.D. grant support). Delphine Queveau, Luc Guihard, and Christophe Couedel are thanked for their technical support.
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Mouneim, H., Besbes, E., Jury, V. et al. Combined Effects of Baking Conditions and Bacterial α-Amylases on Staling Kinetics of Degassed and Porous Bread Crumb. Food Bioprocess Technol 5, 3032–3041 (2012). https://doi.org/10.1007/s11947-011-0716-2
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DOI: https://doi.org/10.1007/s11947-011-0716-2