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OsbZIP23 and OsbZIP45, members of the rice basic leucine zipper transcription factor family, are involved in drought tolerance

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Abstract

Many stress-inducible genes including the transcription factor basic leucine zipper (bZIP) are involved in the response of plants to environmental stresses. bZIPs are composed of two domains, a basic region for DNA binding and a leucine zipper region for dimerization. In this study, two drought-induced bZIP genes OsbZIP23 and OsbZIP45 were identified in rice. The transcription factors are orthologs of Arabidopsis bZIPs belonging to groups A and G, respectively, and are known to be involved in drought tolerance. To investigate the regulation of OsbZIP23 and OsbZIP45 expression in rice, quantitative RT-PCR was performed using RNAs from plants grown at drought stress conditions and different developmental stages. Expression of OsbZIP23 and OsbZIP45 showed positive correlation with drought tolerance. To further understand the functions of OsbZIP23 and OsbZIP45, we overexpressed OsbZIP23 and OsbZIP45 in rice using PGD1 promoter. Results of phenotypic and chlorophyll fluorescence analysis on PGD1:OsbZIP23 and PGD1:OsbZIP45 plants showed enhanced tolerance to drought stress. These results suggest that OsbZIP23 and OsbZIP45 are involved in drought stress response in rice and have a great potential for engineering drought-tolerant crops.

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Acknowledgments

This research was supported by the Rural Development Administration under the Next-Generation BioGreen 21 Program (Project No. PJ011135 to J.-K.K.). This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (Project No. R1A2A1A11051690 to J.-K. K., Project No. R1A1A3012706 to S.-H. P. and Project No. R1A1A1012877 to J. S. J). This work was also supported by Research Resettlement Fund for the new faculty of Seoul National University (J.-K. K).

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    Authors and Affiliations

    1. Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, Pyeongchang, 232-916, Korea

      Su-Hyun Park, Jin Seo Jeong, Kang Hyun Lee, Youn Shic Kim, Yang Do Choi & Ju-Kon Kim

    2. Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Korea

      Yang Do Choi

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    1. Su-Hyun Park
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    2. Jin Seo Jeong
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    Correspondence to Ju-Kon Kim.

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    S.-H. Park, J. S. Jeong and K. H. Lee contributed equally to this work.

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    Park, SH., Jeong, J.S., Lee, K.H. et al. OsbZIP23 and OsbZIP45, members of the rice basic leucine zipper transcription factor family, are involved in drought tolerance. Plant Biotechnol Rep 9, 89–96 (2015). https://doi.org/10.1007/s11816-015-0346-7

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