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Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds

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Abstract

Accelerated ageing is an accurate test indicator of seed vigor and storability that helps to understand the mechanisms of cellular and biochemical deterioration that occur during seed ageing. This study was carried out to elucidate the mechanisms of ageing in macaw palm embryos. Seeds were artificially aged during 4, 8 and 12 days at 45 °C and 100% relative humidity. After ageing, seeds were tested for viability (tetrazolium), electrical conductivity, lipid peroxidation (MDA) and hydrogen peroxide (H2O2) content. Part of the aged seeds was imbibed for 8 days and then determined the hydrogen peroxide content and the activity of antioxidant system enzymes (superoxide dismutase, catalase and glutathione reductase). Ageing reduced the embryo viability from 8 days of treatment and increased malondialdehyde content (MDA) and solute leakage. Hence, membrane permeability correlated with both loss of viability and lipid peroxidation. Imbibition after ageing significantly increased H2O2 content along with superoxide dismutase activity. Catalase activity was significantly higher than control in embryos aged from 8 days and imbibed, and glutathione reductase activity did not change. Our results suggest that macaw palm seed deterioration during accelerated ageing is closely related to lipid peroxidation, and that enzymatic antioxidant system is not completely efficient in reducing reactive oxygen species after imbibition, a critical phase to germination. Moreover, accelerated ageing test can be used as a reliable model to understand the mechanisms involved in palm seeds deterioration.

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References

  • Aebi H (1984) Oxygen radicals in biological systems. Method Enzymol 105:121–126

    Article  CAS  Google Scholar 

  • Ahmad P, Sarwat M, Sharma S (2008) Reactive oxygen species, antioxidants and signaling in plants. J Plant Biol 51:167–173

    Article  CAS  Google Scholar 

  • Al-Maskri AY, Khan MM, Al-Habsi K (2003) Effect of accelerated ageing on viability, vigor (rgr), lipid peroxidation and leakage in carrot (Daucus carota L.) seeds. Int J Agric Biol 5:580–584

    Google Scholar 

  • Arc E, Ogé L, Grappin P, Rajjou L (2011) Plant seed: a relevant model to study aging processes. In: Olgun A (ed) The field of biological aging: past, present and future. Transworld Research Network, India, pp 87–102

    Google Scholar 

  • Azevedo Filho J, Colombo C, Berton L (2012) Macaúba: palmeira nativa como opção bioenergética. Pesqui Tecnol 9:1–10

    Google Scholar 

  • Bailly C (2004) Active oxygen species and antioxidants in seed biology. Seed Sci Res 14:93–107

    Article  CAS  Google Scholar 

  • Bailly C, Benamar A, Corbineau F, Come D (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiol Plant 97:104–110

    Article  CAS  Google Scholar 

  • Bailly C, Benamar A, Corbineau F, Côme D (1998) Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol Plant 104:646–652

    Article  CAS  Google Scholar 

  • Bailly C, Bogatek-Leszczynska R, Côme D, Corbineau F (2002) Changes in activities of antioxidant enzymes and lipoxygenase during growth of sunflower seedlings from seeds of different vigour. Seed Sci Res 12:47–55

    Article  CAS  Google Scholar 

  • Bailly C, El-Maarouf-Bouteau H, Corbineau F (2008) From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. C R Biol 331:806–814

    Article  CAS  PubMed  Google Scholar 

  • Barreto LC, Garcia QS, Morales M, Müller M, Munné-Bosch S (2014) Vitamin E and defense-related phytohormones are reliable markers of embryo growth in macaw palm fruits exposed to various storage conditions. Plant Cell Tissue Organ 118:203–213

    Article  CAS  Google Scholar 

  • Barreto LC, Magalhães ALL, Takahashi JA, Garcia QS (2016) Dynamic of reserve compounds of mesocarp and seeds of macaw palm (Acrocomia aculeata) submitted to different storage conditions. Trees. doi:10.1007/s00468-016-1423-4

  • Belletti P, Lanteri S, Lotito S (1991) The influence of temperature and moisture on seed ageing in Iceland poppy (Papaver nudicaule L.). Sci Hortic 48:153–158

    Article  Google Scholar 

  • Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566

    Article  CAS  PubMed  Google Scholar 

  • Bhattachrjee S (2005) Reactive oxygen species and oxidative burst: roles in stress, senescence and signal transduction in plant. Curr Sci 89:1113–1121

    Google Scholar 

  • Bicalho EM, Pintó-Marijuan M, Morales M, Müller M, Munné-Bosch S, Garcia QS (2015) Control of macaw palm seed germination by the gibberellin/abscisic acid balance. Plant Biol 17:990–996

    Article  CAS  PubMed  Google Scholar 

  • Blokhina O (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Cakmak I, Horst WJ (1991) Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant 83:463–468

    Article  CAS  Google Scholar 

  • Corbineau F, Picard MA, Fougereux JA, Ladonne F, Côme D (2000) Effects of dehydration conditions on desiccation tolerance of developing pea seeds as related to oligosaccharide content and cell membrane properties. Seed Sci Res 10:329–339

    CAS  Google Scholar 

  • Ellis R, Hong T (1991) Seed storage behaviour in Elaeis guineensis. Seed Sci Res 1:99–104

    Google Scholar 

  • Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25

    Article  CAS  PubMed  Google Scholar 

  • Galleschi L, Capocchi A, Ghiringhelli S, Saviozzi F, Calucci L, Pinzino C, Zandomeneghi M (2002) Antioxidants, free radicals, storage proteins, and proteolytic activities in wheat (Triticum durum) seeds during accelerated aging. J Agric Food Chem 50:5450–5457

    Article  CAS  PubMed  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases. Plant Physiol 59:309–314

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930

    Article  CAS  PubMed  Google Scholar 

  • Goel A, Goel AK, Sheoran IS (2003) Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J Plant Physiol 160:1093–1100

    Article  CAS  PubMed  Google Scholar 

  • Gomes MP, Garcia QS (2013) Reactive oxygen species and seed germination. Biologia 68:351–357

    CAS  Google Scholar 

  • Gordin CRB, Scalon SDPQ, Masetto TE (2015) Accelerated aging test in niger seeds. J Seed Sci 37:234–240

    Article  Google Scholar 

  • Hay FR, Merritt DJ, Soanes JA, Dixon KW (2010) Comparative longevity of Australian orchid (Orchidaceae) seeds under experimental and low temperature storage conditions. Bot J Linn Soc 164:26–41

    Article  Google Scholar 

  • Hendry GAF (1993) Oxygen, free radical processes and seed longevity. Seed Sci Res 3:141–153

    Article  CAS  Google Scholar 

  • Hiane PA, Ramos MM, Ramos MIL, Macedo MLR (2005) Óleo da polpa e amêndoa de bocaiúva, Acrocomia aculeata (Jacq.) Lodd. Caracterização e composição em ácidos graxos. Braz J Food Tech 8:256–259

    CAS  Google Scholar 

  • Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611

    Article  CAS  Google Scholar 

  • Hossain MA, Bhattacharjee S, Armin SM, Qian P, Xin W, Li HY, Burritt DJ, Fujita M, Tran LSP (2015) Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Front Plant Sci 6:1–19

    Google Scholar 

  • Kibinza S, Vinel D, Côme D, Bailly C, Corbineau F (2006) Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiol Plant 128:496–506

    Article  CAS  Google Scholar 

  • Krainart C, Siri B, Vichitphan K (2015) Effects of accelerated aging and subsequent priming on seed quality and biochemical change of hybrid cucumber (Cucumis sativa Linn.) seeds. J Agric Technol 11:165–179

    CAS  Google Scholar 

  • Kumar JSP, Prasad RS, Banerjee R, Thammineni C (2015) Seed birth to death: dual functions of reactive oxygen species in seed physiology. Ann Bot 116:663–668

    Article  Google Scholar 

  • Lehner A, Mamadou N, Poels P, Côme D, Bailly C, Corbineau F (2008) Changes in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activities in the embryo during ageing in wheat grains. J Cereal Sci 47:555–565

    Article  CAS  Google Scholar 

  • Leprince O, Hendry GAF, McKersie BD (1993) The mechanisms of desiccation tolerance in developing seeds. Seed Sci Res 3:231–246

    Article  Google Scholar 

  • Liu R, Lai T, Xu Y, Tian S (2013) Changes in physiology and quality of Laiyang pear in long time storage. Sci Hortic 150:31–36

    Article  CAS  Google Scholar 

  • McDonald MB (1999) Seed deterioration: physiology, repair and assessment. Seed Sci Technol 27:177–237

    Google Scholar 

  • Merritt DJ, Senaratna T, Touchell DH, Dixon KW, Sivasithamparam K (2003) Seed ageing of four Western Australian species in relation to storage. Seed Sci Res 13:155–165

    Article  Google Scholar 

  • Meyer AJ (2008) The integration of glutathione homeostasis and redox signaling. J Plant Physiol 165:1390–1403

    Article  CAS  PubMed  Google Scholar 

  • Mira S, Estrelles E, González-Benito ME, Corbineau F (2011) Biochemical changes induced in seeds of Brassicaceae wild species during ageing. Acta Physiol Plant 33:1803–1809

    Article  CAS  Google Scholar 

  • Moura E, Ventrella M, Motoike S (2010) Anatomy, histochemistry and ultrastructure of seed and somatic embryo of Acrocomia aculeata (Arecaceae). Sci Agric 67:399–407

    Article  Google Scholar 

  • Murugesan P, Mathur RK, Pillai RSN, Babu MK (2005) Effect of accelerated aging on seed germination of oil palm (Elaeis guineensis Jacq. var. dura Becc.). Seed Technol 27:108–112

    Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Negreiros GF, Perez SCJG (2004) Resposta fisiológica de sementes de palmeiras ao envelhecimento acelerado. Pesqui Agropecu Bras 39:391–396

    Article  Google Scholar 

  • Noctor G, Mhamdi A, Chaouch S, Han Y, Neukerman J, Marquez-Garcia B, Queval G, Foyer CH (2012) Glutathione in plants: an integrated overview. Plant Cell Environ 35:454–484

    Article  CAS  PubMed  Google Scholar 

  • Nonogaki H, Bassel GW, Bewley JD (2010) Germination—still a mystery. Plant Sci 179:574–581

    Article  CAS  Google Scholar 

  • Priestley DA, Leopold AC (1979) Absence of lipid oxidation during accelerated aging of soybean seeds. Plant Physiol 63:726–729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ratajczak E, Pukacka S (2005) Decrease in beech (Fagus sylvatica) seed viability caused by temperature and humidity conditions as related to membrane damage and lipid composition. Acta Physiol Plant 27:3–12

    Article  CAS  Google Scholar 

  • Ratajczak E, Małecka A, Bagniewska-Zadworna A, Kalemba EM (2015) The production, localization and spreading of reactive oxygen species contributes to the low vitality of long-term stored common beech (Fagus sylvatica L.) seeds. J Plant Physiol 174:147–156

    Article  CAS  PubMed  Google Scholar 

  • Ribeiro LM, Garcia QS, Oliveira DMT, Neves SC (2010) Critérios para o teste de tetrazólio na estimativa do potencial germinativo em macaúba. Pesqui Agropecu Bras 45:361–368

    Article  Google Scholar 

  • Ribeiro LM, Neves SC, Silva PO, Andrade IG (2011) Germinação de embriões zigóticos e desenvolvimento in vitro de coquinho-azedo. Revista Ceres 58:133–139

    Article  Google Scholar 

  • Roberts EH, Ellis RH (1989) Water and seed survival. Ann Bot 63:39

    Article  Google Scholar 

  • Rubio Neto A, Silva FG, Sales JF, Pires LL, Freitas BSM, Souza AL (2015) Effects of drying temperature on viability of macaw palm (Acrocomia aculeata) zygotic embryos. Afr J Biotechnol 14:319–326

    Article  Google Scholar 

  • Silva PO, Ribeiro LM, Simões MOM, Lopes SN, Farias TM, Garcia QS (2013) Fruit maturation and in vitro germination of macaw palm embryos. Afr J Biotechnol 12:446–452

    Article  Google Scholar 

  • Stewart RR, Bewley JD (1980) Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol 65:245–248

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sung JM, Jeng TL (1994) Lipid peroxidation and peroxide-scavenging enzymes associated with accelerated aging of peanut seed. Physiol Plant 91:51–55

    Article  CAS  Google Scholar 

  • Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Sci 151:59–66

    Article  CAS  Google Scholar 

  • Walters C (1998) Understanding the mechanisms and kinetics of seed aging. Seed Sci Res 8:223–244

    Article  CAS  Google Scholar 

  • Walters C, Wheeler LM, Grotenhuis JM (2005) Longevity of seeds stored in a genebank: species characteristics. Seed Sci Res 15:1–20

    Article  CAS  Google Scholar 

  • Wang F, Wang R, Jing W, Zhang W (2011) Quantitative dissection of lipid degradation in rice seeds during accelerated aging. Plant Growth Regul 66:49–58

    Article  Google Scholar 

  • Wojtyla Ł, Garnczarska M, Zalewski T, Bednarski W, Ratajczak L, Jurga S (2006) A comparative study of water distribution, free radical production and activation of antioxidative metabolism in germinating pea seeds. J Plant Physiol 163:1207–1220

    Article  CAS  PubMed  Google Scholar 

  • Xia F, Chen L, Sun Y, Mao P (2015) Relationships between ultrastructure of embryo cells and biochemical variations during ageing of oat (Avena sativa L.) seeds with different moisture content. Acta Physiol Plant 37:1–11

    Article  CAS  Google Scholar 

  • Zacheo G, Cappello AR, Perrone LM, Gnoni GV (1998) Analysis of factors influencing lipid oxidation of almond seeds during accelerated ageing. Leb Wiss Technol 31:6–9

    Article  CAS  Google Scholar 

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Acknowledgements

We thank Dr. Luzia Valentino Modolo for providing reagents and equipment and Elisa Monteze Bicalho for the support on enzyme analyses. Financial support for this research was received through FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) L.C.B. received fellowship from FAPEMIG and Q.S.G. received research productivity scholarships from CNPq.

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  1. Departamento de Botânica, Universidade Federal de Minas, Gerais, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, Brazil

    Leilane Carvalho Barreto & Queila Souza Garcia

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  1. Leilane Carvalho Barreto
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  2. Queila Souza Garcia
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Correspondence to Leilane Carvalho Barreto.

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Communicated by A. Gniazdowska-Piekarska.

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Barreto, L.C., Garcia, Q.S. Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds. Acta Physiol Plant 39, 72 (2017). https://doi.org/10.1007/s11738-017-2367-z

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