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Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing

  • Somaclonal Variation
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Abstract

The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping of 11 microsatellite loci showed high levels of genomic instability and a high frequency of mutation in the obtained megagametophyte-derived callus cultures. All cultures contained new mutations in one or more microsatellite loci.

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Acknowledgments

This study was supported by Research Grant No. 14.Y26.31.0004 from the Government of the Russian Federation.

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Authors and Affiliations

  1. Department of Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Georg-August-University of Göttingen, Büsgenweg 2, 37077, Göttingen, Germany

    Konstantin V. Krutovsky

  2. Department of Ecosystem Science and Management, Texas A&M University, College Station, TX, 77843-2138, USA

    Konstantin V. Krutovsky

  3. N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina, Moscow, 119333, Russia

    Konstantin V. Krutovsky

  4. Genome Research and Education Center, Siberian Federal University, 50a/2 Akademgorodok, Krasnoyarsk, 660036, Russia

    Konstantin V. Krutovsky, Iraida N. Tretyakova, Nataliay V. Oreshkova & Eugene A. Vaganov

  5. Laboratory of Forest Genetics and Selection, V. N. Sukachev Institute of Forest, Siberian Branch of Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russia

    Iraida N. Tretyakova, Nataliay V. Oreshkova, Maria E. Pak & Olga V. Kvitko

  6. Department of Ecology and Environmental Studies, Institute of Economics, Management and Environmental Studies, Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russia

    Eugene A. Vaganov

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  1. Konstantin V. Krutovsky
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Krutovsky, K.V., Tretyakova, I.N., Oreshkova, N.V. et al. Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing. In Vitro Cell.Dev.Biol.-Plant 50, 655–664 (2014). https://doi.org/10.1007/s11627-014-9619-z

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