Skip to main content
SpringerLink
Log in

In vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae)

  • Micropropagation
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

The organogenic potential of root explants derived from cultured seedlings of Bixa orellana L. (annatto) was investigated in response to different incubation conditions and either 4.44 μM 6-benzyladenine, 4.54 μM Thidiazuron, or 4.56 μM Zeatin. Explants cultured in liquid media with agitation generally showed better development of adventitious buds versus explants cultured on semi-solid media. The most adventitious buds developed from explants cultured in liquid media under a 16-h photoperiod. Use of Zeatin and Thidiazuron promoted the development of more adventitious buds than 6-benzyladenine but morphological abnormalities among regenerating shoots and plants were observed. Fewer adventitious buds developed from explants cultured in liquid media supplemented with 6-benzyladenine, but the buds gave rise to the highest percentage of morphologically normal regenerated plants. Histological analyses showed that adventitious buds originated from cell proliferation within the pericycle, opposite the protoxylem poles of the explant. Seedling root tissue is useful for in vitro propagation of B. orellana.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.

Similar content being viewed by others

References

  • Aasim M.; Hussain N.; Umer E. M.; Zubair M.; Hussain S. B.; Saeed S.; Rafique T. S.; Sancak C. In vitro shoot regeneration of fenugreek (Trigonella foenum-graecum L.) using different cytokinins. Afr. J. Biotechnol. 9: 7174–7179; 2010.

    CAS  Google Scholar 

  • Ahmad N.; Siddique I.; Anis M. Improved plant regeneration in Capsicum annuum L. from nodal segments. Biol. Plant. 50: 701–704; 2006. doi:10.1007/s10535-006-0110-5.

    Article  CAS  Google Scholar 

  • Almeida J. L.; Almeida F. C. G.; Nunes R. P.; Almeida F. A. G. Bud initiation in leaf explants of annatto seedlings on different cytokinins. Cienc. Rural. 26: 45–49; 1996.

    Google Scholar 

  • Aparnathi K.; Lata R.; Sharma R. Annatto (Bixa orellana L.): its cultivation, preparation and usage. IJTA 8: 80–88; 1990.

    Google Scholar 

  • Arora K.; Sharma M.; Srivastava J.; Ranade S. A.; Sharma A. K. In vitro cloning of Azadirachta indica from root explants. Biol. Plant. 55: 164–168; 2011. doi:10.1007/s10535-011-0023-9.

    Article  CAS  Google Scholar 

  • Atta R.; Laurens L.; Boucheron-Dubuisson E.; Guivarch A.; Carnero E.; Giraudat-Pautot V.; Rech P.; Chriqui D. Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro. Plant J. 57: 626–644; 2009. doi:10.1111/j.1365-313X.2008.03715.x.

    Article  CAS  PubMed  Google Scholar 

  • Blakesey D. Uptake and metabolism of 6-benzyladenine in shoot proliferation of Musa and Rhododendron. Plant Cell Tissue Organ Cult. 25: 69–74; 1991. doi:10.1007/BF00033915.

    Article  Google Scholar 

  • Bouvier F.; Dogbo O.; Camara B. Biosynthesis of the food and cosmetic plant pigment bixin (Annatto). Science 300: 2089–2091; 2003. doi:10.1126/science.1085162.

    Article  CAS  PubMed  Google Scholar 

  • Carvalho J. F. R. P.; Carvalho C. R.; Otoni W. C. Regeneração in vitro de urucum (Bixa orellana L.) a partir de diferentes tipos de explantes. Rev. Árvore 29: 887–895; 2005.

    Article  Google Scholar 

  • Cruz C. D. Programa Genes: Análise multivariada e simulação. Editora UFV, Viçosa; 2006. 175p.

    Google Scholar 

  • D’Souza M. C.; Sharon M. In vitro clonal propagation of annatto (Bixa orellana L.). In Vitro Cell. Dev. Biol. Plant 37: 168–172; 2001. doi:10.1007/s11627-001-0029-7.

    Article  Google Scholar 

  • Debata B. K.; Punk F. Micropropagation of Bixa orellana L. In: Breeding research on medicinal and aromatics plants. Proceedings 2: 334–337; 1996

  • Evert R. F. Esau’s Plant Anatomy, meristems, cells, and tissues of the plant body: their structure, function, and development. 3rd ed. Wiley, New Jersey; 2006.

    Book  Google Scholar 

  • Franklin G.; Sheeba C. J.; Lakshmi Sita G. Regeneration of eggplant (Solanum melongena L.) from root explants. In Vitro Cell. Dev. Biol. Plant 40: 188–191; 2004. doi:10.1079/IVP2003491.

    Article  Google Scholar 

  • Gamborg O. L.; Miller R. A.; Ojima K. Nutrient requirement of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158; 1968. doi:10.1016/0014-4827(68)90403-5.

    Article  CAS  PubMed  Google Scholar 

  • Gordon S. P.; Heisler M. G.; Reddy G. V.; Ohno C.; Das P.; Meyerowitz E. M. Pattern formation during de novo assembly of the Arabidopsis shoot meristem. Development 134: 3539–3548; 2007. doi:10.1242/dev.010298.

    Article  CAS  PubMed  Google Scholar 

  • Ivanova M.; Staden J. V. Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla. Plant Cell Tissue Organ Cult. 104: 13–21; 2011. doi:10.1007/s11240-010-9794-5.

    Article  CAS  Google Scholar 

  • Joseph N.; Siril E. A.; Nair G. M. An efficient in vitro propagation methodology for Annatto (Bixa orellana L.). Physiol. Mol. Biol. Plants 3: 263–270; 2011. doi:10.1007/s12298-011-0073-7.

    Article  Google Scholar 

  • Karnovsky M. J. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27: 137–138; 1965.

    Google Scholar 

  • Lombardi S. P.; Passos I. R. S.; Nogueira M. C. S.; Appezzato-da-Glória B. In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata Mast. Braz. Arch. Biol. Technol. 50: 239–247; 2007. doi:10.1590/S1516-89132007000200009.

    Article  Google Scholar 

  • Magyar-Tábori K.; Dobránski J.; Teixeira da Silva J. A.; Bulley S. M.; Hudák I. The role of cytokinins in shoot organogenesis in apple. Plant Cell Tissue Organ Cult. 101: 251–267; 2010. doi:10.1007/s11240-010-9696-6.

    Article  Google Scholar 

  • Mahendranath G.; Venugopalan A.; Parimalan R.; Giridhar P.; Ravishankar G. A. Annatto pigment production in root cultures of Achiote (Bixa orellana L.). Plant Cell Tissue Organ Cult. 106: 517–522; 2011. doi:10.1007/s11240-011-9931-9.

    Article  CAS  Google Scholar 

  • Mallón R.; Rodríguez-Oubiña J.; González M. L. Shoot regeneration from in vitro-derived leaf and root explants of Centaurea ultreiae. Plant Cell Tissue Organ Cult. 106: 523–530; 2011. doi:10.1007/s11240-011-9934-6.

    Article  Google Scholar 

  • Mehrotra S.; Goel M. K.; Kukreja A. K.; Mishra B. N. Efficiency of liquid culture systems over conventional micropropagation: A progress towards commercialization. Afr. J. Biotechnol. 6: 1484–1492; 2007.

    CAS  Google Scholar 

  • Murashige T.; Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497; 1962. doi:10.1111/j.1399-3054.1962.tb08052.x.

    Article  CAS  Google Scholar 

  • Nef-Campa C.; Chaintreuil-Dongmo C.; Dreyfus B. L. Regeneration of the tropical legume Aeschynomene sensitive Sw. from root explants. Plant Cell Tissue Organ Cult. 44: 149–154; 1996. doi:10.1007/BF00048193.

    Article  Google Scholar 

  • O’Brien T. P.; McCully M. E. The study of plant structure principles and selected methods. Termarcarphi Pty, Melbourne; 1981.

    Google Scholar 

  • Paiva Neto V. B.; Botelho M. N.; Aguiar R.; Silva E. A. M.; Otoni W. C. Somatic embryogenesis from immature zygotic embryos of annatto (Bixa orellana L.). In Vitro Cell. Dev. Biol. Plant 39: 629–634; 2003a. doi:10.1079/IVP2003465.

    Article  Google Scholar 

  • Paiva Neto V. B.; Mota T. R.; Otoni W. C. Direct organogenesis from hypocotyl-derived explants of annatto (Bixa orellana). Plant Cell Tissue Organ Cult. 75: 159–167; 2003b. doi:10.1023/A:1025063906822.

    Article  Google Scholar 

  • Parimalan R.; Giridhar P.; Gururaj H. B.; Ravishankar G. A. Organogenesis from cotyledon and hypocotyls-derived explants of japhara (Bixa orellana L.). Acta Bot. Croat. 66: 153–160; 2007.

    Google Scholar 

  • Parimalan R.; Giridhar P.; Ravishankar G. A. Mass multiplication of Bixa orellana L. through tissue culture for commercial propagation. Ind. Crops. Prod. 28: 122–127; 2008. doi:10.1016/j.indcrop.2008.01.012.

    Article  CAS  Google Scholar 

  • Parimalan R.; Venugopalan A.; Giridhar P.; Ravishankar G. A. Somatic embryogenesis and Agrobacterium-mediated transformation in Bixa orellana L. Plant Cell Tissue Organ Cult. 105: 317–328; 2011. doi:10.1007/s11240-010-9870-x.

    Article  CAS  Google Scholar 

  • Park S. Y.; Murthy H. N.; Paek K. Y. Protocorm-like body induction and subsequent plant regeneration from root tip cultures of Doritaenopsis. Plant Sci. 164: 919–923; 2003. doi:10.1016/S0168-9452(03)00019-0.

    Article  CAS  Google Scholar 

  • Parveen S.; Shahzad A. A micropropagation protocol for Cassia angustifolia Vahl. from root explants. Acta Physiol. Plant. 33: 789–796; 2011. doi:10.1007/s11738-010-0603-x.

    Article  Google Scholar 

  • Perilli S.; Moubayidin L.; Sabatini S. The molecular basis of cytokinin function. Curr. Opin. Plant Biol. 13: 21–26; 2010. doi:10.1016/j.pbi.2009.09.018.

    Article  CAS  PubMed  Google Scholar 

  • Preston H. D.; Rickard M. D. Extraction and chemistry of annatto. Food Chem. 5: 47–56; 1980. doi:10.1016/0308-8146(80)90063-1.

    Article  CAS  Google Scholar 

  • Ramamurthy N.; Savithramma N.; Usha R.; Swamy P. M. Multiple shoot induction and regeneration of japhara (Bixa orellana L.) through axillary bud derived callus cultures. J. Plant Biol 26: 231–235; 1999.

    Google Scholar 

  • Ranyaphiaa R. A.; Maoaa A. A.; Borthakurb S. K. Direct organogenesis from leaf and internode explants of in vitro raised wintergreen plant (Gaultheria fragrantissima). Sci. Asia 37: 186–194; 2011. doi:10.2306/scienceasia1513-1874.2011.37.186.

    Article  Google Scholar 

  • Riefler M.; Novak O.; Strnad M.; Schmülling T. Arabidopsis cytokinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root development, and cytokinin metabolism. Plant Cell 18: 40–54; 2006. doi:10.1105/tpc.105.037796.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Rivera-Madrid R.; Escobedo R. M. G. M.; Balam-Galera E.; Vera-ku M.; Harries H. Preliminary studies toward genetic improvement of annatto (Bixa orellana L.). Sci. Hortic. 106: 165–172; 2006. doi:10.1016/j.scienta.2006.03.011.

    Article  Google Scholar 

  • Rocha D. I.; Vieira L. M.; Tanaka F. A. O.; Silva L. C.; Otoni W. C. Anatomical and ultrastructural analyses of in vitro organogenesis from root explants of commercial passion fruit (Passiflora edulis Sims). Plant Cell Tissue Organ Cult. 111: 69–78; 2012. doi:10.1007/s11240-012-0171-4.

    Article  CAS  Google Scholar 

  • Sahai A.; Shahzad A.; Sharma S. Histology of organogenesis and somatic embryogenesis in excised root cultures of an endangered species Tylophora indica (Asclepiadaceae). Aust. J. Bot. 58: 198–205; 2010. doi:10.1071/BT09220.

    Article  Google Scholar 

  • Sandal I.; Bhattacharya A.; Ahuja P. S. An efficient liquid culture system for tea shoot proliferation. Plant Cell Tissue Organ Cult. 65: 75–80; 2001. doi:10.1023/A:1010662306067.

    Article  CAS  Google Scholar 

  • Sha Valli Khan P. S.; Prakash E.; Rao K. R. Callus induction and plantlet regeneration in Bixa orellana L., an annatto-yelding tree. In Vitro Cell. Dev. Biol. Plant 38: 186–190; 2002. doi:10.1079/IVP2001284.

    Article  Google Scholar 

  • Silva C. V.; Oliveira L. S.; Loriato V. A. P.; Silva L. C.; Campos J. M. S.; Viccini L. F.; Oliveira E. J.; Otoni W. C. Organogenesis from root explants of commercial populations of Passiflora edulis Sims and a wild passion fruit species, P. cincinnata Masters. Plant Cell Tissue Organ Cult. 107: 407–416; 2011. doi:10.1007/s11240-011-9991-x.

    Article  Google Scholar 

  • Simões C.; Albarello N.; Callado C. H.; Castro T. C.; Mansur E. New approaches for shoot production and establishment of in vitro root cultures of Cleome rosea Vahl. Plant Cell Tissue Organ Cult. 98: 79–86; 2009. doi:10.1007/s11240-009-9540.

    Article  Google Scholar 

  • Siril E. A.; Joseph N. Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers. Physiol. Mol. Biol. Plants 19: 147–155; 2013. doi:10.1007/s12298-012-0150-6.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Smet I.; Vanneste S.; Inzé D.; Beeckman T. Lateral root initiation or the birth of a new meristem. Plant Mol. Biol. 60: 871–887; 2006. doi:10.1007/s11103-005-4547-2.

    Article  CAS  PubMed  Google Scholar 

  • Smith M. A. L.; Spomer L. A. Vessels, gels, liquid media, and support systems. In: Aitken-Christie J.; Kozai T.; Smith M. A. L. (eds) Automation and environmental control in plant tissue culture. Kluwer, Dordrecht, pp 371–404; 1995.

    Chapter  Google Scholar 

  • Su Y. H.; Liu Y. B.; Zhang X. S. Auxin-cytokinin interaction regulates meristem development. Mol. Plant 4: 616–625; 2011. doi:10.1093/mp/ssr007.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Venugopalan A.; Giridhar P.; Ravishankar G. A. Food, ethanobotanical and diversified applications of Bixa orellana L.: A scope for its improvement through biotechnological mediation. Indian J. Appl. Life Sci. 1: 9–31; 2011.

    Google Scholar 

  • Vila S.; Gonzalez A.; Rey H.; Mroginski L. Plant regeneration, origin, and development of shoot buds from root segments of Melia azedarach L. (Meliaceae) seedlings. In Vitro Cell. Dev. Biol. Plant 41: 746–751; 2005. doi:10.1079/IVP2005692.

    Article  CAS  Google Scholar 

  • Vinocur B.; Carmi T.; Altman A.; Ziv M. Enhanced bud regeneration in aspen (Populus tremula L.) root cultured in liquid media. Plant Cell Rep. 19: 1146–1154; 2000. doi:10.1007/s002990000243.

    Article  CAS  Google Scholar 

  • Yang J. L.; Seong E. S.; Kim M. J.; Ghimire B. K.; Kang W. H.; Yu C. Y.; Li C. H. Direct somatic embryogenesis from pericycle cells of broccoli (Brassica oleracea L. var. italica) root explants. Plant Cell Tissue Organ Cult. 100: 49–58; 2010. doi:10.1007/s11240-009-9615-x.

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the following Brazilian sponsoring agencies for the financial support: FAPEMIG (Belo Horizonte, MG), CAPES (Brasília, DF), and CNPq (Brasília, DF).

Author information

Authors and Affiliations

  1. Departmento de Biologia Vegetal, Laboratório de Cultura de Tecidos/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Av. P.H. Rolfs, 36570-000, Viçosa, MG, Brazil

    Ana Claudia Ferreira da Cruz, Diego Ismael Rocha, Lourdes Iarema & Wagner Campos Otoni

  2. Departmento de Biologia Vegetal, Laboratório de Anatomia Vegetal, Universidade Federal de Viçosa, Campus Universitário, Av. P.H. Rolfs, 36570-000, Viçosa, MG, Brazil

    Marília Contim Ventrella

  3. Departamento de Ciências Biológicas, Centro de Biotecnologia e Genética, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, Salobrinho, 45662-000, Ilhéus, BA, Brazil

    Marcio Gilberto Cardoso Costa

  4. Universidade Federal do Mato Grosso do Sul, Centro de Ciências Agrárias, Campus Universitário de Chapadão do Sul, 79560-000, Chapadão do Sul, MS, Brazil

    Vespasiano Borges Paiva Neto

  5. Plant Biology Department/BIOAGRO, Federal University of Viçosa, P.H. Rolfs Avenue, s/n, University Campus, 36570-000, Viçosa, MG, Brazil

    Wagner Campos Otoni

Authors
  1. Ana Claudia Ferreira da Cruz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Diego Ismael Rocha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lourdes Iarema
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marília Contim Ventrella
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marcio Gilberto Cardoso Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vespasiano Borges Paiva Neto
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wagner Campos Otoni
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wagner Campos Otoni.

Additional information

Editor: J. Finer

Rights and permissions

Reprints and permissions

About this article

Cite this article

da Cruz, A.C.F., Rocha, D.I., Iarema, L. et al. In vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae). In Vitro Cell.Dev.Biol.-Plant 50, 76–83 (2014). https://doi.org/10.1007/s11627-013-9580-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-013-9580-2

Keywords

Search

Navigation