Summary
Efficient shoot regeneration of sugarcane (Saccharum spp. hybrid cv. CP84-1198) from embryogenic callus cultures has been obtained using thidiazuron (TDZ). Callus was placed on modified Murashige and Skoog (MS) medium containing 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D), or 9.3 μM kinetin and 22.3 μM naphthaleneacetic acid (NAA) and compared with the same MS medium supplemented with 0.5, 1.0, 2.5, 5.0 or 10.0 μMTDZ, A11 TDZ treatments resulted in faster shoot regeneration than the kinetin/NAA treatment, and more shoot production than either the 2,4-D or kinetin/NAA treatments. Maximum response, as determined by total number of shoots (26 per explant) and number of shoots greater than 1 cm (4 per explant) 4 wk after initiation, was obtained with 1.0 μM TDZ. The shoots rooted efficiently on MS medium supplemented with 19.7 μM indole-3-butyric acid (IBA). These results indicate that TDZ effectively stimulates sugarcane plant regeneration from embryogenic callus, and may be suitable to use in genetic transformation studies to enhance regeneration of transgenic plants.
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References
Ahloowalia, B. S.; Maretzki, A. Plant regeneration via somatic embryogenesis in sugarcane. Plant Cell Rep. 2:21–25; 1983.
Arencibia, A; Vázquez, R. I.; Prieto, D. L.; Téllez, P.; Carmona, E. R.; Coego, A.; Hernández, L.; De la Riva, G. A.; Selman-Housein, G. Transgenic sugarcane plants resistant to stem borer attack. Mol. Breed. 3:247–255; 1997.
Bates, S.; Preece, J. E.; Navarrete, N. E.; Van Sambeek, J. W.; Gaffney, G. R. Thidiazuron stimulates shoot organogenesis and somatic embryogenesis in white ash (Fraxinus americana L.). Plant Cell Tiss. Org. Cult. 31:21–29; 1992.
Bower, R.; Birch, R. G. Transgenic sugarcane plants via microprojectile bombardment. Plant J. 2:409–416; 1992.
Enrìquez-Obregón, G. A.; Vázquez-Padrón, R. I.; Prieto-Samsonov, D. L.; De la Riva, G. A.; Selman-Housein, G. Herbicide-resistant sugarcane (Saccharum officinarum L.) plants by Agrobacterium-mediated transformation. Planta 206:20–27; 1998.
Faure, O.; Diemer, F.; Moja, S.; Jullien, F. Mannitol and thidiazuron improve in vitro shoot regeneration from spearmint and peppermint leaf disks. Plant Cell Tiss. Org. Cult. 52:209–212; 1998.
Fitch, M. M. M.; Moore, P. H. Comparison of 2,4-D and picloram for selection of long-term totipotent green callus cultures of sugarcane. Plant Cell Tiss. Org. Cult. 20:157–163; 1990.
Gallo-Meagher, M.; Irvine, J. E. Herbicide resistant transgenic sugarcane plants containing the bar gene. Crop. Sci. 36:1367–1374; 1996.
Gupta, S. D.; Conger, B. V. In vitro differentiation of multiple shoot clumps from intact seedlings of switchgrass. In Vitro Cell. Dev. Biol.-Plant. 34:196–202; 1998.
Heinz, D. J.; Mee, G. W. P. Plant differentiation from callus tissue of Saccharum species. Crop Sci. 9:346–348: 1969.
Heinz, D. J.; Krishnamurthi, M.; Nickell, L. G., Maretski, Cell, tissue and organ culture in sugarcane improvement. In: Reiner, J.; Bajaj, V. P. S., eds. Applied fundamental aspects of plant cell, tissue and organ culture. Berlin: Springer-Verlag; 1977;3–17.
Heinz, D. J.; Mee, G. W. P. Morphological, cytogenetic, and enzymatic variation in Saccharum species hybrid clones derived from callus Tissue. Am. J. Bot. 58:257–262;1971.
Ho, W. J.; Vasil, I. K. Somatic embryogenesis in sugarcane (Saccharum officinarum L.). I. The morphology and physiology of callus formation and the ontogeny of somatic embryos. Protoplasma 118:169–180; 1983.
Huetteman, C. A.; Preece, J. E. Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33:105–119; 1993.
Ingelbrecht, I. L.; Irvine, J. E.; Mirkov, T. E. Posttranscriptional gene silencing in transgenic sugarcane. Dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome. Plant Physiol. 119:1187–1197; 1999.
Irvine, J. E.; Benda, G. T. A. Transmission of sugar cane diseases in plants derived by rapid regeneration from diseased leaf tissue. Sugar Cane. 6 (Nov/Dec):14–16; 1987.
Irvine, J. E.; Benda, G. T. A.; Legendre, B. L.; Machado, Jr., G. R. The frequency of marker changes in sugarcane plants regenerated from callus culture. II. Evidence for vegetative and genetic transmission, epigenetic effects and chimeral disruption. Plant Cell Tiss. Org. Cult. 26:115–125; 1991.
Liu, M.-C.; Sugarcane. In: Sharp, W. H.; Evans, D.A.; Ammirato, P. V.; Yamada, Y., eds. Handbook of plant cell culture. Vol. 2. New York: MacMillan; 1984;572–605.
Magioli, C.; Rocha, A. P. M.; de Oliveira, D. E.; Mansur, E.; Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. Plant Cell Rep. 17:661–663; 1998.
Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Ihidiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cell. Dev. Biol.-Plant. 34:267–275; 1998.
Nickell, L. G., Crop improvement in sugarcane: studies using in vitro methods. Crop Sci. 17:717–719; 1977.
SAS Institute. SAS/STAT guide for personal computers, version 6.12 ed. Cany, NC: SAS Institute; 1996.
Sauer, J. D. Historical geography of crop plants: a select roster. Boca Raton, FL: Lewis Publishers; 1993;236–250.
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Gallo-Meagher, M., English, R.G. & Abouzid, A. Thidiazuron stimulates shoot regeneration of sugarcane embryogenic callus. In Vitro Cell.Dev.Biol.-Plant 36, 37–40 (2000). https://doi.org/10.1007/s11627-000-0009-3
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DOI: https://doi.org/10.1007/s11627-000-0009-3