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Successful cultivation of a valuable wild strain of Lepista sordida from Thailand

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Abstract

Lepista sordida is an edible and medicinal mushroom, but, until now, it had to be collected from the wild. The present study is the first report of the successful cultivation of a wild strain of L. sordida from Thailand. The morphological description and molecular examination of the fungus are included, in order to confirm the identification of the species. Optimization was carried out for mycelium growth and fruiting body production. yeast malt extract (YMA), pH 6.0 - 7.0 and a temperature of 25 - 30 °C. A sorghum mushroom spawn was used for upscaling of the mycelium to be used for fruiting body production. Optimal conditions for the fruiting phase were 25 °C with 95–97% humidity in a compost rice straw medium with sandy-soil casing layer. Additionally, the secondary metabolites of fruiting body and cultured mycelium were investigated. Nudic acid B, a known toxic polyacetylene, was isolated from submerged cultures of L. sordida, while no polyacetylenic compounds were found in the fruiting bodies.

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Acknowledgements

Financial support by the German Academic Exchange Service (DAAD) and the Thai Royal Golden Ph.D. Jubilee-Industry program (RGJ) for a joint TRF-DAAD PPP academic exchange grant to B.T., K.D.H., and M.S., and the RGJ for a personal stipend no. Ph.D/0138/2553 in 4.S.MF/53/A.3 to B.T. is gratefully acknowledged. K.D.H. would like to thank the Thailand Research Fund for a grant (BRG5580009) to study the taxonomy, phylogeny, and biochemistry of Thai basidiomycetes. Sequences from the University of Wyoming were provided with funding from NSF grant DEB-1050292 to S.L.M. The help of Anan Thawthong and Kritsana Jatuwong at Mae Fah Luang University in carrying out mushrooms harvesting is acknowledged.

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    Authors and Affiliations

    1. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

      Benjarong Thongbai, Kevin D. Hyde, Naritsada Thongklang & Ekachai Chukeatirote

    2. Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), and German Centre for Infection Research (DZIF), partner site Hannover/Braunschweig, Inhoffenstrasse 7, 38124, Braunschweig, Germany

      Kathrin Wittstein, Christian Richter & Marc Stadler

    3. Botany Department, University of Wyoming, Laramie, WY, 82071-3165, USA

      Steven L. Miller

    4. Rak Ruai Mushroom Farm, Phetchabun, 67160, Thailand

      Namphung Klomklung

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    1. Benjarong Thongbai
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    Correspondence to Marc Stadler.

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    Section Editor: Zhu-Liang Yang

    This article is part of the “Special Issue in honour of the 70th birthday of Dr. Eric McKenzie”.

    Benjarong Thongbai and Christian Richter contributed equally to this work.

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    Thongbai, B., Wittstein, K., Richter, C. et al. Successful cultivation of a valuable wild strain of Lepista sordida from Thailand. Mycol Progress 16, 311–323 (2017). https://doi.org/10.1007/s11557-016-1262-0

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