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In vivo assessment of polydatin, a natural polyphenol compound, on arsenic-induced free radical overproduction, gene expression, and genotoxicity

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Abstract

Arsenic (As) is a well-known contaminant of global groundwater. Its exposure causes several hazardous effects on animals and human via oxidative stress. The present study examined the effect of polydatin (PD) on free radical overproduction in rats exposed to As. Thirty-five male rats randomly allocated into five equal groups. To the control group, physiological saline was given orally and to the second group only 100 mg/L As was given by drinking water for 60 days. The other groups were treated with As (100 mg/L) and PD orally at 50, 100, and 200 mg/kg/day, respectively. Treatment with As enhanced malondialdehyde level but decreased glutathione level in blood, liver, kidney, brain, lung, and heart of rats. Also, As decreased superoxide dismutase and catalase activities of erythrocyte, liver, kidney, brain, lung, and heart in rats. Furthermore, As treatment gave rise to increased DNA damage and gene expressions of interleukin 1 beta (IL-1β), nuclear factor kappa beta (NFκB), p53, and tumor necrosis factor-α (TNF-α) in the lung, brain, kidney, and liver. However, treatment of PD ameliorated As-exposed lipid peroxidation, antioxidant enzymes activities, DNA damage, gene expressions, and histopathological changes in tissues. In conclusion, PD has a dose-dependent protective effect on lipid peroxidation and antioxidant defense mechanism in rats against As exposure.

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Funding

This study was supported by a grant from the Afyon Kocatepe University Scientific Research Council, Afyonkarahisar, Turkey (Project no: 15.VF.07).

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

    Damla Arslan-Acaroz & Ismail Kucukkurt

  2. Department of Molecular Biology and Genetics, Faculty of Art and Science, Bartin University, 74100, Bartin, Turkey

    Fahriye Zemheri

  3. Bayat Vocational School, Afyon Kocatepe University, Bayat, Afyonkarahisar, Turkey

    Hasan Huseyin Demirel

  4. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

    Sinan Ince

  5. Department of Physiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

    Abdullah Eryavuz

Authors
  1. Damla Arslan-Acaroz
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  2. Fahriye Zemheri
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  3. Hasan Huseyin Demirel
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  4. Ismail Kucukkurt
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  5. Sinan Ince
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  6. Abdullah Eryavuz
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Corresponding author

Correspondence to Sinan Ince.

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Responsible editor: Philippe Garrigues

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Arslan-Acaroz, D., Zemheri, F., Demirel, H.H. et al. In vivo assessment of polydatin, a natural polyphenol compound, on arsenic-induced free radical overproduction, gene expression, and genotoxicity. Environ Sci Pollut Res 25, 2614–2622 (2018). https://doi.org/10.1007/s11356-017-0391-6

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  • DOI: https://doi.org/10.1007/s11356-017-0391-6

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