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Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani

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Abstract

Several volatile organic compounds (VOCs) producing endophyte bacteria were isolated from the leaves of olive trees and tested for their antifungal activity against several pathogenic fungi. An antagonistic strain called P2 showed 97 % of homology with Pseudomonas sp. strains on the basis of its 16S rDNA sequence and biochemical properties. P2 strain drastically inhibited the growth of Rhizoctonia solani mycelia (86 %) at 5 day-post-confrontation (dpc) and strongly reduced fungi infection on potato slices at 107 bacteria ml−1 for 3 and 7 dpc. P2 strain was also positive for protease activity as well as siderophore production. Light microscopy analysis showed that treatment of R. solani mycelia with P2 strain induced thickening of the cell-wall, vesiculation of protoplasm and blockage of fungal hyphae branching. VOCs analysis using GC–MS allowed the detection of two major products with m/z of 93.9910 and 125.9630 corresponding to dimethyl disulfide and dimethyl trisulfide respectively. VOCs-producing P2 strain could be a promising agent in the protection of tuber crops against fungal diseases.

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Acknowledgments

This work was financially supported by grants from the Tunisian Ministry of Higher Education, Scientific Research, Technology Information and Communication. We would like to thank Prof. Ezzedine Aouani for critically reading the manuscript.

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Authors and Affiliations

  1. Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj-Cédria, BP-901, 2050, Hammam-Lif, Tunisia

    Salem Elkahoui, Najeh Yaich, Sana Azaiez, Majdi Hammami, Rym Essid & Ferid Limam

  2. Laboratoire de Physiologie Moléculaire des Plantes, Centre de Biotechnologie, Borj-Cédria, BP-901, 2050, Hammam-Lif, Tunisia

    Naceur Djébali

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  1. Salem Elkahoui
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  2. Naceur Djébali
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  3. Najeh Yaich
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  7. Ferid Limam
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Correspondence to Ferid Limam.

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Elkahoui, S., Djébali, N., Yaich, N. et al. Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani . World J Microbiol Biotechnol 31, 175–185 (2015). https://doi.org/10.1007/s11274-014-1772-3

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  • DOI: https://doi.org/10.1007/s11274-014-1772-3

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