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Genetic variation and phylogenetic analysis of porcine circovirus type 2 infections in central China

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Abstract

Porcine circovirus type 2 (PCV2) infection causes postweaning multisystemic wasting syndrome and porcine circovirus-associated diseases in many regions. A total of 77 sequences, including 31 sampled from Henan province of China, were retrieved from GenBank and subjected to amino acid variation and phylogenetic analyses. The two PCV genotypes prevailing in Henan were PCV-2a and PCV-2b with PCV-2b accounting for 93.5 % (29/31) of the Henan isolates. The 31 Henan isolates all shared between 92.7 and 100 % sequence similarity. Amino acid variation analysis of the capsid protein revealed that Henan PCV2 strains tended to accumulate more substitutions within epitopic regions—a substitution pattern consistent with host immune system-mediated selection of virus immune escape variants. The analysed PCV sequences carry evidence of at least six unique recombination events. Selective pressure analysis of the relative recombination-free ORF2 sequences of these viruses revealed evidence of sites that are likely evolving in response to host-driven immune pressures—a finding that coupled with information on the prevalent diversity in Henan PCV2 isolates of known immunoreactive genomic loci will aid in future studies aiming to assess the evolutionary responses of PCV2 in China to the widespread deployment of anti-PCV vaccines in the country.

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Acknowledgements

This study was supported by the National Nature Science Foundation of China (No. 31172346).We are grateful to Professor Darren P. Martin in University of Cape Town for technical support. We would like to thank the reference sequence submitters for their original works.

Conflict of interest

None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, No. 95. Wenhua Road, Zhengzhou, 450002, China

    Chunlong Mu, Qingyuan Yang, Yina Zhang, Yonghui Zhou, Jixi Zhang, Pingan Xia & Baoan Cui

  2. Institute of Infectious Diseases and Molecular Medicine, Computational Biology Group, University of Cape Town, Cape Town, 7579, South Africa

    Darren P. Martin

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  1. Chunlong Mu
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  2. Qingyuan Yang
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  3. Yina Zhang
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  4. Yonghui Zhou
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Correspondence to Pingan Xia or Baoan Cui.

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Mu, C., Yang, Q., Zhang, Y. et al. Genetic variation and phylogenetic analysis of porcine circovirus type 2 infections in central China. Virus Genes 45, 463–473 (2012). https://doi.org/10.1007/s11262-012-0789-7

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  • DOI: https://doi.org/10.1007/s11262-012-0789-7

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