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Genetic analysis of porcine circovirus type 2 (PCV2) strains isolated between 2001 and 2009: genotype PCV2b predominate in postweaning multisystemic wasting syndrome occurrences in eastern China

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Abstract

Porcine circovirus type 2 (PCV2) is the essential infectious agent of postweaning multisystemic wasting syndrome (PMWS). In the present study, we obtained sequences of 31 PCV2 isolates from different farms of 11 provinces of eastern China and analyzed the genetic characterization of 136 eastern China-derivate PCV2 isolated during 2001–2009. The results showed that these PCV2 isolates could be divided into two groups, PCV2b (108 of 1A/1B, 19 of 1C) and PCV2a (1 of 2A, 2 of 2D, 6 of 2E). Among the 9 PCV2a isolates, eight were found before the year 2005. Meanwhile, three major heterogenic regions were observed in amino acid positions 53–91, 121–151, and 190–210; a few specific substitution patterns were found in each subgroup and several variant or conserved epitopes were also observed in the Cap protein. It indicated that PCV2b predominated in PMWS occurrences in eastern China and a few recombinants of different genotypes existed in nature.

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References

  1. G. Allan, J. Ellis, J. Vet. Diagn. Invest. 12, 3–14 (2000)

    CAS  PubMed  Google Scholar 

  2. J. Segale′s, G.M. Allan, M. Domingo, Anim. Health Res. Rev. 6, 119–142 (2005)

    Article  Google Scholar 

  3. A.L. Hamel, L.L. Lin, G.P. Nayar, J. Virol. 72, 5262–5267 (1998)

    CAS  PubMed  Google Scholar 

  4. B.M. Meehan, F. McNeilly, D. Todd, S. Kennedy, V.A. Jewhurst, J.A. Ellis, L.E. Hassard, E.G. Clark, D.M. Haines, G.M. Allan, J. Gen. Virol. 79, 2171–2179 (1998)

    CAS  PubMed  Google Scholar 

  5. A. Mankertz, M. Domingo, J.M. Folch, P. LeCann, A. Jestin, J. Segales, B. Chmielewicz, J. Plana-Duran, D. Soike, Virus Res. 66, 65–77 (2000)

    Article  CAS  PubMed  Google Scholar 

  6. A. Olvera, M. Cortey, J. Segales, Virology 357, 175–185 (2007)

    Article  CAS  PubMed  Google Scholar 

  7. A.K. Cheung, L.M. Lager, O.I. Kohutyuk, A.L. Vincent, S.C. Henry, R.B. Baker, R.R.R. Rowland, A.G. Dunham, Arch. Virol. 152, 1035–1044 (2007)

    Article  CAS  PubMed  Google Scholar 

  8. C. de Boisseson, V. Beven, L. Bigarre, R. Thiery, N. Rose, E. Eveno, F. Madec, A. Jestin, J. Gen. Virol. 85, 293–304 (2004)

    Article  PubMed  Google Scholar 

  9. S.S. Grierson, G.P. King, G.J. Wellenberg, M. Banks, Res. Vet. Sci. 77, 265–268 (2004)

    Article  CAS  PubMed  Google Scholar 

  10. C.M. Ma, C.C. Hon, T.Y. Lam, V.Y. Li, C.K. Wong, T. de Oliveira, F.C. Leung, J. Gen. Virol. 88, 1733–1737 (2007)

    Article  CAS  PubMed  Google Scholar 

  11. J. Segale′s, A. Olvera, L. Grau-Roma, C. Charreyre, H. Nauwynck, L. Larsen, K. Dupont, K. McCullough, J. Ellis, S. Krakowka, A. Mankertz, M. Fredholm, C. Fossum, S. Timmusk, N. Stockhofe-Zurwieden, V. Beattie, D. Armstrong, B. Grassland, P. Baekbo, G. Allan, Vet. Rec. 162(26), 867–868 (2008)

    Google Scholar 

  12. L. Wen, X. Guo, H. Yang, Vet. Microbiol. 110, 141–146 (2005)

    Article  CAS  PubMed  Google Scholar 

  13. J. Shuai, W. Wei, X. Li, N. Chen, Z. Zhang, X. Chen, W. Fang, Virus Genes 35, 619–627 (2007)

    Article  CAS  PubMed  Google Scholar 

  14. F. Wang, X. Guo, X. Ge, Z. Wang, Y. Chen, Z. Cha, H. Yang, Virus Res. 145, 151–156 (2009)

    Article  CAS  PubMed  Google Scholar 

  15. J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 25, 4876–4882 (1997)

    Article  CAS  PubMed  Google Scholar 

  16. K. Tamura, J. Dudley, M. Nei, S. Kumar, Mol. Biol. Evol. 24, 1596–1599 (2007)

    Article  CAS  PubMed  Google Scholar 

  17. S. Shang, Y. Jin, X. Jiang, J. Zhou, X. Zhang, G. Xing, J. He, Y. Yan, Mol. Immunol. 46, 327–334 (2009)

    Article  CAS  PubMed  Google Scholar 

  18. S. Carman, B. McEwen, J. DeLay, H. Cari, J. Fairles, Can. Vet. J. 47(8), 761–762 (2006)

    PubMed  Google Scholar 

  19. A.M. Martins Gomes de Castro, A. Cortez, M.B. Heinemann, P.E. Brandao, L.J. Richtzenhain, Arch. Virol. 152(8), 1435–1445 (2007)

    Article  CAS  PubMed  Google Scholar 

  20. S. Timmusk, P. Wallgren, K. Belak, M. Berg, C. Fossum, in Proceeding of ESVV International Conference on Animal Circoviruses and Associated Diseases, Belfast, 2005, p. 82

  21. R. Larochelle, R. Magar, S. D’Allaire, Virus Res. 90, 101–112 (2002)

    Article  CAS  PubMed  Google Scholar 

  22. D. Todd, A.N. Scott, N.W. Ball, B.J. Borghmans, B.M. Adair, J. Virol. 76, 8472–8474 (2002)

    Article  CAS  PubMed  Google Scholar 

  23. L. Stevenson, F. McNeilly, C. Duffy, I. McNair, B. Adair, G. Allan, in Proceedings of the Fifth International Symposium on Emerging and Re-emerging Pig Diseases, Krakau, Poland, 2007, p. 51

  24. K. Dupont, E.O. Nielsen, P. Bækbo, L.E. Larsen, Vet. Microbiol. 128, 56–64 (2008)

    Article  CAS  PubMed  Google Scholar 

  25. L. Grau-Roma, E. Crisci, M. Sibila, S. Lo′pez-Soria, M. Nofrarias, M. Cortey, L. Fraile, A. Olvera, J. Segale′s, Vet. Microbiol. 128, 23–35 (2008)

    Article  CAS  PubMed  Google Scholar 

  26. D.J. An, I.S. Roh, D.S. Song, C.K. Park, B.K. Park, Virus Res. 129, 115–122 (2007)

    Article  CAS  PubMed  Google Scholar 

  27. D. Mahe, P. Blanchard, C. Truong, C. Arnauld, P. Le Cann, R. Cariolet, F. Madec, E. Albina, A. Jestin, J. Gen. Virol. 81, 1815–1824 (2000)

    CAS  PubMed  Google Scholar 

  28. P. Lekcharoensuk, I. Morozov, P.S. Paul, N. Thangthumniyom, W. Wajjawalku, X.J. Meng, J. Virol. 78, 8135–8145 (2004)

    Article  CAS  PubMed  Google Scholar 

  29. Y. Ha, K. Jung, C. Choi, K.K. Hwang, C. Chae, J. Comp. Pathol. 133, 201–204 (2005)

    Article  CAS  PubMed  Google Scholar 

  30. Q. Liu, S.K. Tikoo, S.K.L.A. Babiuk, Virology 285, 91–99 (2001)

    Article  CAS  PubMed  Google Scholar 

  31. C. Truong, D. Mahe, P. Blanchard, M. Le Dimna, F. Madec, A. Jestin, E. Albina, Arch. Virol. 146, 1197–1211 (2001)

    Article  CAS  PubMed  Google Scholar 

  32. D.J. Lefebvre, J.V. Doorsselaere, P.L. Delputte, H.J. Nauwynck, Arch. Virol. 154, 875–879 (2009)

    Article  CAS  PubMed  Google Scholar 

  33. A.K. Cheung, Arch. Virol. 154, 531–534 (2009)

    Article  CAS  PubMed  Google Scholar 

  34. R. Hesse, M. Kerrigan, R.R. Rowland, Virus Res. 132, 201–207 (2008)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by National key technology R&D program (2006BAD6A07), grants from the National Natural Science Foundation (30871868), Jiangsu province Natural Science Foundation (BK2005093), and partly Foundation for Ph.D. students training program, Ministry of Education (20060307012).

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Authors and Affiliations

  1. Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Wenliang Li, Xianwei Wang, Tao Ma, Zhixin Feng, Yufeng Li & Ping Jiang

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  1. Wenliang Li
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  2. Xianwei Wang
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  3. Tao Ma
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  4. Zhixin Feng
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  5. Yufeng Li
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Correspondence to Ping Jiang.

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Li, W., Wang, X., Ma, T. et al. Genetic analysis of porcine circovirus type 2 (PCV2) strains isolated between 2001 and 2009: genotype PCV2b predominate in postweaning multisystemic wasting syndrome occurrences in eastern China. Virus Genes 40, 244–251 (2010). https://doi.org/10.1007/s11262-009-0438-y

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  • DOI: https://doi.org/10.1007/s11262-009-0438-y

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