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Expression of CphB- and CphE-type cyanophycinases in cyanophycin-producing tobacco and comparison of their ability to degrade cyanophycin in plant and plant extracts

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Abstract

Increasing the arginine (Arg) content in plants used as feed or food is of interest, since the supplementation of food with conditionally essential Arg has been shown to have nutritional benefits. An increase was achieved by the expression of the Arg-rich bacterial storage component, cyanophycin (CGP), in the chloroplast of transgenic plants. CGP is stable in plants and its degradation into β-aspartic acid (Asp)-Arg dipeptides, is solely catalyzed by bacterial cyanophycinases (CGPase). Dipeptides can be absorbed by animals even more efficiently than free amino acids (Matthews and Adibi 1976; Wenzel et al. 2001). The simultaneous production of CGP and CGPase in plants could be a source of β-Asp-Arg dipeptides if CGP degradation can be prevented in planta or if dipeptides are stable in the plants. We have shown for the first time that it is possible to co-express CGP and CGPase in the same plant without substrate degradation in planta by transient expression of the cyanobacterial CGPase CPHB (either in the plastid or cytosol), and the non-cyanobacterial CGPase CPHE (cytosol) in CGP-producing Nicotiana tabacum plants. We compared their ability to degrade CGP in planta and in crude plant extracts. No CGP degradation appeared prior to cell homogenization independent of the CGPase produced. In crude plant extracts, only cytosolic CPHE led to a fast degradation of CGP. CPHE also showed higher stability and in vitro activity compared to both CPHB variants. This work is the next step to increase Arg in forage plants using a stable, Arg-rich storage protein.

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Acknowledgements

We would like to express our very great appreciations to Alex Rajewski for proof-reading and the helpful suggestions and Dr. Martin Krehenbrink, Cysal GmbH, for sharing their aquaculture results.

Funding

This study was funded by the University of Rostock.

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Authors and Affiliations

  1. Department of Agrobiotechnology and Risk Assessment for Bio- and Gene Technology, Faculty of Agricultural and Environmental Sciences, University of Rostock, Justus-von-Liebig Weg 8, 18059, Rostock, Germany

    Daniel Ponndorf, Inge Broer & Henrik Nausch

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  1. Daniel Ponndorf
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  2. Inge Broer
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  3. Henrik Nausch
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Correspondence to Inge Broer.

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Ponndorf, D., Broer, I. & Nausch, H. Expression of CphB- and CphE-type cyanophycinases in cyanophycin-producing tobacco and comparison of their ability to degrade cyanophycin in plant and plant extracts. Transgenic Res 26, 491–499 (2017). https://doi.org/10.1007/s11248-017-0019-0

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