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Utilization of the plant methionine sulfoxide reductase B genes as selectable markers in Arabidopsis and tomato transformation

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Abstract

Plant transformation is an important tool for basic research and agricultural biotechnology. In most cases, selection of putative transformants is based on antibiotic or herbicide resistance. Overexpression of plant genes that provide protection from abiotic or biotic stresses can result in a conferred phenotype that can be used as a means for selection. We have demonstrated herein that specific methionine sulfoxide reductase B (MsrB) genes that are overexpressed in transgenic plants may constitute a new selectable marker with concomitantly increased tolerance to methyl viologen (MV) treatment. Arabidopsis transformants overexpressing cytosolic MsrB7, MsrB8 or MsrB9 are viable and survive after MV selection. To establish whether these native plant origin genes serve as new non-antibiotic markers that can be applied to crop transformation, tomato cotyledons were used as transformation materials. MsrB7 transgenic tomato plants were successfully obtained by Agrobacterium-mediated transformation and selection on medium supplemented with MV. We suggest that specific MsrB genes that are overexpressed in transgenic plants may constitute a new selectable marker with increased tolerance to oxidative stress concomitant with MV treatment.

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Abbreviations

CaMV :

Cauliflower mosaic virus

Hpt :

Hygromycin phosphotransferase

MS:

Murashige and Skoog

MsrB:

Methionine sulfoxide reductase B

MV:

Methyl viologen

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Acknowledgments

We thank Mr. Jent-Turn Lee and Ms. Pei-Shan Chieh for technical support in identification of some of the overexpressing transgenic Arabidopsis lines. We thank Dr. Choun-Sea Lin and Ms. Fu-Hui Wu for technical support with the tomato transformation. We thank Drs. Kahwee Koh, Venkatesh Prasad, and Ms. Miranda Loney for helpful discussions and grammar correction. This work was supported by a grant from Academia Sinica, Grant (98-2324-B-001-003-CC1) from the Development Program of Industrialization for Agricultural Biotechnology of the Republic of China and grant (99AS-1.1.1-FD-Z1) from the Agriculture and Food Agency, Council of Agriculture, Executive Yuan of Republic of China.

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Authors and Affiliations

  1. Department of Biotechnology, TransWorld University, Douliu City, 640, Yunlin County, Taiwan

    Chia-Wen Li

  2. Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, 741, Taiwan

    Shu-Hong Lee & Ming-Tsair Chan

  3. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan

    Shu-Hong Lee & Ming-Tsair Chan

  4. Institute of Biotechnology, National Cheng Kung University, Tainan, 701, Taiwan

    Shu-Hong Lee & Ming-Tsair Chan

Authors
  1. Chia-Wen Li
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  2. Shu-Hong Lee
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  3. Ming-Tsair Chan
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Correspondence to Ming-Tsair Chan.

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Chia-Wen Li, Shu-Hong Lee contributed equally to this work.

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Li, CW., Lee, SH. & Chan, MT. Utilization of the plant methionine sulfoxide reductase B genes as selectable markers in Arabidopsis and tomato transformation. Plant Cell Tiss Organ Cult 113, 555–563 (2013). https://doi.org/10.1007/s11240-013-0296-0

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  • DOI: https://doi.org/10.1007/s11240-013-0296-0

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