Skip to main content
SpringerLink
Log in

A Novel Little Membrane Protein Confers Salt Tolerance in Rice (Oryza sativa L.)

  • Original Paper
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Plasma membrane proteins play critical roles in sensing and responding abiotic and biotic stresses in plants. In the present study, we characterized a previously unknown gene stress associated little protein 1 (SALP1) encoding a plasma membrane protein. SALP1, a small and plant-specific membrane protein, contains only 74 amino acid residues. SALP1 was constitutively expressed in various rice tissues while highly expressed in roots, leaf blade, and immature panicles. Expression analysis indicated that SALP1 was induced by various abiotic stresses and abscisic acid (ABA). Subcellular localization assay indicated that SALP1 was localized on plasma membrane in rice protoplast cells. Overexpressing of SALP1 in rice improved salt tolerance through increasing free proline contents and the expression level of OsP5CS gene, and balancing ion contents under salt stress. Moreover, SALP1 transgenic rice showed reduced sensitivity to ABA treatment, and expression level of SALP1 is not altered by ABI5-like 1 protein. Conclusively, SALP1, a novel membrane protein, is involved in salt tolerance through an ABA-independent signaling pathway in rice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Adie BA, Perez-Perez J, Perez-Perez MM, Godoy M, Sanchez-Serrano JJ, Schmelz EA, Solano R (2007) ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis. Plant Cell 19(5):1665–1681. doi:10.1105/tpc.106.048041

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bassil E, Tajima H, Liang YC, Ohto MA, Ushijima K, Nakano R, Blumwald E (2011) The Arabidopsis Na+/H+ antiporters NHX1 and NHX2 control vacuolar pH and K+ homeostasis to regulate growth, flower development, and reproduction. Plant Cell 23(9):3482–3497. doi:10.1105/tpc.111.089581

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chen Z, Hartmann HA, Wu MJ, Friedman EJ, Chen JG, Pulley M, Jones AM (2006) Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins. Plant Mol Biol 60(4):583–597. doi:10.1007/s11103-005-5082-x

    Article  CAS  PubMed  Google Scholar 

  • Guo L, Wang ZY, Lin H, Cui WE, Chen J, Liu M, Gu H (2006) Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family. Cell Res 16(3):277–286. doi:10.1038/sj.cr.7310035

    Article  CAS  PubMed  Google Scholar 

  • Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6(2):271–282

    Article  CAS  PubMed  Google Scholar 

  • Hu CA, Delauney AJ, Verma DP (1992) A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. Proc Natl Acad Sci U S A 89(19):9354–9358

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huang J, Yang X, Wang M, Tang H, Ding L, Shen Y, Zhang H (2007) A novel rice C2H2-type zinc finger protein lacking DLN-box/EAR-motif plays a role in salt tolerance. Biochim Biophys Acta 1769(4):220–227. doi:10.1016/j.bbaexp.2007.02.006

    Article  CAS  PubMed  Google Scholar 

  • Huang J, Sun S, Xu D, Yang X, Bao Y, Wang Z, Zhang H (2009) Increased tolerance of rice to cold, drought and oxidative stresses mediated by the overexpression of a gene that encodes the zinc finger protein ZFP245. Biochem Biophys Res Commun 389(3):556–561. doi:10.1016/j.bbrc.2009.09.032

    Article  CAS  PubMed  Google Scholar 

  • Huang J, Sun S, Xu D, Lan H, Sun H, Wang Z, Zhang H (2012) A TFIIIA-type zinc finger protein confers multiple abiotic stress tolerances in transgenic rice (Oryza sativa L.). Plant Mol Biol 80(3):337–350. doi:10.1007/s11103-012-9955-5

    Article  CAS  PubMed  Google Scholar 

  • Igarashi Y, Yoshiba Y, Sanada Y, Yamaguchi-Shinozaki K, Wada K, Shinozaki K (1997) Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L. Plant Mol Biol 33(5):857–865

    Article  CAS  PubMed  Google Scholar 

  • Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345(2):646–651. doi:10.1016/j.bbrc.2006.04.140

    Article  CAS  PubMed  Google Scholar 

  • Lu G, Gao C, Zheng X, Han B (2009) Identification of OsbZIP72 as a positive regulator of ABA response and drought tolerance in rice. Planta 229(3):605–615. doi:10.1007/s00425-008-0857-3

    Article  CAS  PubMed  Google Scholar 

  • Preston GM, Carroll TP, Guggino WB, Agre P (1992) Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256(5055):385–387

    Article  CAS  PubMed  Google Scholar 

  • Reece KS, McElroy D, Wu R (1990) Genomic nucleotide sequence of four rice (Oryza sativa) actin genes. Plant Mol Biol 14(4):621–624

    Article  CAS  PubMed  Google Scholar 

  • Sanderfoot A (2007) Increases in the number of SNARE genes parallels the rise of multicellularity among the green plants. Plant Physiol 144(1):6–17. doi:10.1104/pp. 106.092973

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sokolovski S, Hills A, Gay RA, Blatt MR (2008) Functional interaction of the SNARE protein NtSyp121 in Ca2+ channel gating, Ca2+ transients and ABA signalling of stomatal guard cells. Mol Plant 1(2):347–358. doi:10.1093/mp/ssm029

    Article  CAS  PubMed  Google Scholar 

  • Sun SJ, Guo S, Yang X, Bao Y, Tang HJ, Sun H, Zhang H (2010) Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in rice. J Exp Bot 61(10):2807–2818. doi:10.1093/jxb/erq120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sun H, Huang X, Xu X, Lan H, Huang J, Zhang H (2012) ENAC1, a NAC transcription factor, is an early and transient response regulator induced by abiotic stress in rice (Oryza sativa L.). Mol Biotechnol 52(2):101–110. doi:10.1007/s12033-011-9477-4

    Article  CAS  PubMed  Google Scholar 

  • Troll W, Lindsley J (1955) A photometric method for the determination of proline. J Biol Chem 215(2):655–660

    CAS  PubMed  Google Scholar 

  • Yang X, Yang YN, Xue LJ, Zou MJ, Liu JY, Chen F, Xue HW (2011) Rice ABI5-Like1 regulates abscisic acid and auxin responses by affecting the expression of ABRE-containing genes. Plant Physiol 156(3):1397–1409. doi:10.1104/pp. 111.173427

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang X, Wang J, Huang J, Lan H, Wang C, Yin C, Zhang H (2012) Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice. Proc Natl Acad Sci U S A 109(52):21534–21539. doi:10.1073/pnas.1219776110

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273. doi:10.1146/annurev.arplant.53.091401.143329

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

This work was supported by Jiangsu Collaborative Innovation Center for Modern Crop Production, the National Science Foundation of China (31571627, 31471469), and the Fundamental Research Funds for the Central Universities (no. KYZ201137).

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Xi Yuan, Hui Sun, Zhengbin Tang, Haijuan Tang, Hongsheng Zhang & Ji Huang

  2. Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China

    Xi Yuan, Hui Sun, Zhengbin Tang, Haijuan Tang, Hongsheng Zhang & Ji Huang

Authors
  1. Xi Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hui Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhengbin Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Haijuan Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongsheng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ji Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Hongsheng Zhang or Ji Huang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Fig. S1

Identification of SALP1-OE transgenic rice. The RT-PCR (A) and real-time PCR (B) were employed. (GIF 5 kb)

High resolution image (TIFF 955 kb)

Fig. S2

Transcriptional activity assay of SALP1 in yeast cells. – and + stand for negative (empty vector of pGBKT7) and positive (pGBKT7-53 + pGADT7-T) controls, respectively. (GIF 6 kb)

High resolution image (TIFF 681 kb)

Table S1

Primer sequences used in this study. (DOCX 16 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yuan, X., Sun, H., Tang, Z. et al. A Novel Little Membrane Protein Confers Salt Tolerance in Rice (Oryza sativa L.). Plant Mol Biol Rep 34, 524–532 (2016). https://doi.org/10.1007/s11105-015-0944-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11105-015-0944-0

Keywords

Search

Navigation