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Cadmium-induced ammonium ion accumulation of rice seedlings at high temperature is mediated through abscisic acid

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Abstract

In this study, we examined interaction between cadmium (Cd) and temperature in rice seedlings. Effects of CdCl2 (0.5 mM) simultaneously applied at high (35/30°C day/night), medium (25/20°C) and low (15/13°C) temperatures to rice seedlings were detected by measuring changes in biomass production and NH +4 content. Results indicated that Cd-induced biomass reduction and NH +4 accumulation increased in parallel with temperature increases. On treatment with CdCl2, the abscisic acid (ABA) content markedly increased in the leaves of seedlings grown at high temperature but not at medium and low temperatures. Exogenous application of ABA at medium temperature increased ABA and NH +4 contents in the leaves of rice seedlings. Fluridone (Flu) treatment, an inhibitor of carotenoid biosynthesis, reduced ABA content, as well as Cd-induced NH + 4 accumulation in the leaves of rice seedlings grown at high temperature. These Flu effects can be reversed by application of ABA. Furthermore, Flu treatment did not reduce Cd content in leaves of seedlings grown at high temperature. All these results suggest that Cd-induced NH +4 accumulation at high temperature is mediated through ABA.

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Abbreviations

ABA:

Abscisic acid

DW:

Dry weight

ELISA:

Enzyme-linked immunosorbent assay

Flu:

Fluridone

GS:

Glutamine synthetase

NCED:

9-cis-epoxycarotenoid dioxygenase

PAL :

Phenylalanine ammonia-lyase

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Acknowledgements

This work was supported by a research grant from the National Science Council of the Republic of China.

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Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Yi Ting Hsu, Mei Chun Kuo & Ching Huei Kao

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  1. Yi Ting Hsu
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  2. Mei Chun Kuo
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  3. Ching Huei Kao
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Correspondence to Ching Huei Kao.

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Hsu, Y.T., Kuo, M.C. & Kao, C.H. Cadmium-induced ammonium ion accumulation of rice seedlings at high temperature is mediated through abscisic acid. Plant Soil 287, 267–277 (2006). https://doi.org/10.1007/s11104-006-9076-5

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  • DOI: https://doi.org/10.1007/s11104-006-9076-5

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