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Accumulation of cadmium by hairy-root cultures of Solanum nigrum

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Summary

Cadmium uptake by cultures of transformed hairy-roots of Solanum nigrum was studied. The effect of pH, buffer type, temperature, exposure time and Cd2+ content ranging from 0.2 to 2000 ppm was measured. Cd2+ uptake was dependent on increasing metal concentration and it was time dependent. From the variety of buffers tested, MES buffer and borate ions were found to be beneficial for the Cd2+ uptake. The high effectivness of Cd2+ accumulation in the roots decreased significantly after increasing the Cd2+ content in the buffer over 2 ppm.

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Authors and Affiliations

  1. Department of Plant Tissue Cultures, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo n. 2, 166 10, Prague, Czech Republic

    T. Macek

  2. Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, ICT, Technicka 3, 166 28, Prague, Czech Republic

    P. Kotrba, M. Suchova, K. Demnerova & T. Ruml

  3. Department of Analytical Chemistry, Faculty of Chemical Technology, ICT, Technicka 3, 166 28, Prague, Czech Republic

    F. Skacel

Authors
  1. T. Macek
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  2. P. Kotrba
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  3. M. Suchova
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  4. F. Skacel
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  5. K. Demnerova
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  6. T. Ruml
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Macek, T., Kotrba, P., Suchova, M. et al. Accumulation of cadmium by hairy-root cultures of Solanum nigrum . Biotechnol Lett 16, 621–624 (1994). https://doi.org/10.1007/BF00128611

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  • DOI: https://doi.org/10.1007/BF00128611

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