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Chlorophyll a fluorescence analysis of high-yield rice (Oryza sativa L.) LYPJ during leaf senescence

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Photosynthetica

Abstract

Photosystem II (PSII) photochemistry was examined by chlorophyll (Chl) a fluorescence analysis in high-yield rice LYPJ flag leaves during senescence. Parameters deduced from the JIP-test showed that inhibition of the donor side of PSII was greater than that of the acceptor side in hybrid rice LYPJ. The natural senescence process was accompanied by the increased inactivation of oxygen-evolving complex (OEC) and a lower total number of active reaction centers per absorption. It indicated that the inhibition of electron transport caused by natural senescence might be caused partly by uncoupling of the OEC and/or inactivation of PSII reaction centers. Chl fluorescence parameters analyzed in this study suggested that energy dissipation was enhanced in order to protect senescent leaves from photodamage. Nevertheless, considerably reduced PSI electron transport activity was observed at the later senescence. Thus, natural senescence inhibited OEC-PSII electron transport, but also significantly limited the PSII-PSI electron flow.

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Abbreviations

Chl:

chlorophyll

CS:

cross section

DF:

the total driving force for photosynthesis of the observed system

F0 :

fluorescence intensity at 50 µs

FJ :

fluorescence intensity at the J-step (at 2 ms)

FI :

fluorescence intensity at the I-step (at 30 ms)

FM :

maximal fluorescence intensity

Fv/Fm :

maximum photochemical efficiency of PSII

OEC:

oxygen-evolving complex

P680:

primary electron donor in PSII

PIcs :

the performance index on cross section basis at different times

PItot :

the potential for energy conservation from photons absorbed by PSII to the reduction flux (RE) of PSI end acceptors

PQ:

plastoquinone

RC:

PSII reaction center

tFM :

time to reach FM

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China

    Y. W. Wang, C. Xu, C. F. Lv, M. Wu, X. J. Cai, Z. T. Liu, X. M. Song & G. X. Chen

  2. Zijin College, Nanjing University of Science and Technology, Nanjing, 210023, China

    M. Wu

  3. Institute of Food and Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    C. G. Lv

Authors
  1. Y. W. Wang
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  2. C. Xu
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  3. C. F. Lv
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  4. M. Wu
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  9. C. G. Lv
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Corresponding author

Correspondence to G. X. Chen.

Additional information

Acknowledgments: This research was supported by the National Natural Science Foundation of China (No. 31271621), Natural Science Foundation of the Jiangsu Province, China (No. 11KJA180001), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and NSFC for Talents Training in Basic Science (J1103507, J1210025).

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Wang, Y.W., Xu, C., Lv, C.F. et al. Chlorophyll a fluorescence analysis of high-yield rice (Oryza sativa L.) LYPJ during leaf senescence. Photosynthetica 54, 422–429 (2016). https://doi.org/10.1007/s11099-016-0185-y

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