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Effect of endophyte infection on chlorophyll a fluorescence in salinity stressed rice

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Biologia Plantarum

Abstract

We have earlier reported that the endophyte infection can enhance photosynthetic capacity and antioxidant enzyme activities in rice exposed to salinity stress. Now, the changes in primary photochemistry of photosystem (PS) II induced by Na2CO3 stress in endophyte-infected (E+) and endophyte-uninfected (E-) rice seedlings were studied using chlorophyll a fluorescence (OJIP-test). Performance indices (PIABS and PITotal) of E- and E+ rice seedlings revealed the inhibitory effects of Na2CO3 on PS II connectivity (occurrence of an L-band), oxygen evolving complex (occurrence of a K-band), and on the J step of the induction curves, associated with an inhibition of electron transport from plastoquinone A (QA) to plastoquinone B (QB). In E+ seedlings, Na2CO3 effects on L and K bands were much smaller, or even negligible, and also there was no pronounced effect on the J step. Furthermore, the OJIP parameters indicated that 20 mM Na2CO3 had a greater influence on the photosystem (PS) II electron transport chain than did the 10 mM Na2CO3, and that changes were greater in E- than in E+. Endophyte infection was therefore deemed to enhance the photosynthetic mechanism of Oryza sativa exposed to salinity stress.

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Authors and Affiliations

  1. College of Chemistry and Life Science, Shenyang Normal University, Shenyang, 110034, P.R. China

    X. M. Li, M. J. Chen, J. Li, L. J. Ma, N. Bu & Y. Y. Li

  2. Environmental Science Department of Liaoning University, Shenyang, 110036, P.R. China

    L. H. Zhang

Authors
  1. X. M. Li
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  2. M. J. Chen
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  3. J. Li
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  4. L. J. Ma
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  5. N. Bu
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  6. Y. Y. Li
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  7. L. H. Zhang
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Corresponding authors

Correspondence to X. M. Li or L. H. Zhang.

Additional information

Acknowledgments: This research was financially supported by the National Natural Science Foundation of China (Grant Nos. 31270369 and 31070285), the Director Foundation of Eco-Environmental Research Center at Shenyang Normal University (EERC-K-201302), the Director Foundation of Experimental Center at Shenyang Normal University (SY201102 and SY201104).

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Li, X.M., Chen, M.J., Li, J. et al. Effect of endophyte infection on chlorophyll a fluorescence in salinity stressed rice. Biol Plant 58, 589–594 (2014). https://doi.org/10.1007/s10535-014-0428-3

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  • DOI: https://doi.org/10.1007/s10535-014-0428-3

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