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Increased Expression of Mitochondrial Inner-Membrane Protein Mpv17 After Intracerebral Hemorrhage in Adult Rats

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Abstract

The Mpv17 gene encodes a mitochondrial inner-membrane protein that has been implicated in several cell activities. Almost all studies have previously indicated that loss of function or gene-inactivated in Mpv17 can induce the development of disease. Here, we explored the roles of Mpv17 protein in the pathophysiology of intracerebral hemorrhage (ICH). An ICH rat model was established and assessed by behavioral tests. Using western blot and immunohistochemistry, significant up-regulation of Mpv17 was found in neurons in brain areas surrounding the hematoma following ICH. The increase of Mpv17 expression was found to be accompanied by the enhanced expression of p53, Bax, cytochrome c (Cyt c) and active caspase-3, and decreased expression of Bcl-2 in the pathological process of rat ICH. Furthermore, immunofluorescent staining revealed that Mpv17 co-localized with p53, Bax and active caspase-3 in neurons, suggesting its biological function in the process of neuronal apoptosis. Our in vitro study, using Mpv17 RNA interference in primary cortical neurons, indicated that Mpv17 might exert its anti-apoptotic function in neuronal apoptosis. Thus, Mpv17 may play a role in protecting the brain from secondary damage following ICH.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (No. 81471188). This work is also supported by Nantong Science and Technology project to Aihong Li (No. HS2014032).

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    Authors and Affiliations

    1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China

      Aihong Li, Yaohui Ni & Xin Chen

    2. Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 201602, China

      Xiaomei Chen

    3. Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China

      Aisong Guo

    4. Department of Neurology, The First People’s Hospital of Yancheng, Yancheng, Jiangsu Province, China

      Lei Li

    5. Medical College, Nantong University, Nantong, 226001, Jiangsu, China

      Aihong Li, Lei Li, Xiaolei Sun, Yaohui Ni, Xin Chen & Aisong Guo

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    Aihong Li and Lei Li contributed equally to this work.

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    Li, A., Li, L., Sun, X. et al. Increased Expression of Mitochondrial Inner-Membrane Protein Mpv17 After Intracerebral Hemorrhage in Adult Rats. Neurochem Res 40, 1620–1630 (2015). https://doi.org/10.1007/s11064-015-1644-8

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