Abstract
Bamboo is one of the important plant for pulp, paper and charcoal industries. After China, India is the second largest bamboo reserve in Asia. Around the globe, wide genetic diversity of bamboo is present which serves as the base for selection and improvement. DNA based molecular markers appears to be a striking substitute for systematic assessment of the genetic diversity in conservation and genetic improvement of plants. DNA based molecular markers such as RAPD and ISSR were used to assess the genetic diversity in 13 bamboo genotypes. Total 120 RAPD and 63 ISSR primers were tested, of which only 42 polymorphic primers (30 RAPD and 12 ISSR), gave reproducible amplification profile and were used in this study. 30 RAPD primers yielded total 645 amplified fragments, of which 623 were polymorphic, and 20.76 polymorphic bands per primer were observed across 13 genotypes. 12 ISSR primers produced 246 amplified fragments, of which 241 were polymorphic, and 20.08 polymorphic bands per primer was observed across 13 different genotypes. The Jaccard’s coefficient of RAPD, ISSR and pooled RAPD and ISSR dendrograms ranged from 0.26 to 0.83, 0.23 to 0.86 and 0.26 to 0.84 respectively. The present study found the large genetic diversity present between different elite genotypes of bamboo. Such investigation can deliver a well understanding of the available genotypes, which might be further exploited for the paper industry.
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References
Wang D, Shen SJ (1987) Bamboos of China. Timber Press, Portland
Thomas TA, Arora RK, Singh R (1988) Genetic wealth of bamboos in India and their conservation strategies. Bamboos current research. In: Proceedings of international bamboo workshop, Cochin, 32 Nov 1988, pp 14–18
Loh JP, Kiew R, Set O, Gan LH, Gan YY (2000) A study of genetic variation and relationship within the bamboo subtribe Bambusinae using amplified fragment length polymorphism. Ann Bot 85:607–612
Bahadur KN (1979) Taxonomy of bamboos. Ind J For 2:222–241
Soderstorm TR, Calderon CE (1979) A commentary on bamboos (Poaceae: bambusoideae). Biotropica 11:161–172
Yeasmin L, Ali MN, Gantait S, Chakraborty S (2014) Bamboo: an overview on its genetic diversity and characterization. 3 Biotech. doi:10.1007/s13205-014-0201-5
Stapleton CM, Rao VR (1995) Progress and prospects in genetic diversity studies on bamboo and its conservation. Bamboo, people and the environment. In: Proceedings of Vth international bamboo workshop and the IVth international bamboo congress, Ubud, Bali, 19–22 June 1995
Nayak S, Rout GR, Das P (2003) Evaluation of genetic variability in bamboo using RAPD markers. Plant Soil Environ 49(1):24–28
Goyal AK, Ghosh PK, Dubey PK, Sen A (2012) Inventorying bamboo biodiversity of North Bengal: a case study. Int J Fund Appl Sci 1:5–8
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Gwanama C, Labuschagne MT, Botha AM (2000) Analysis of genetic variation in Cucurbita moschata by random amplified polymorphic DNA (RAPD) markers. Euphytica 113:19–24
Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:225–238
Kapteyn J, Simon JE (2002) The use of RAPDs for assessment of identity, diversity and quality of Echinacea. In: Janick J, Whipkey A (eds) Trends in new crops and new uses. ASHS Press, Alexandria, pp 509–513
Arif M, Zaidi NW, Singh YP, Haq QMR, Singh US (2009) A comparative analysis of ISSR and RAPD markers for study of genetic diversity in Shisham (Dalbergia sissoo). Plant MolBiol Rep 27:488–495
Gajera BB, Kumar N, Singh AS, Punvar BS, Ravikiran R, Subhash N, Jadeja GC (2010) Assessment of genetic diversity in castor (Ricinus communis L.) using RAPD and ISSR markers. Indus Crops Prod 32:491–498
Kong Q, Li X, Xiang C, Wang H, Song J, Zhi H (2011) Genetic diversity of Radish (Raphanus sativus L.) germplasm resources revealed by AFLP and RAPD markers. Plant Mol Biol Rep 29:217–223
Gonzalez A, Coulson A, Brettell R (2002) Development of DNA markers (ISSRs) in mango. Acta Hortic 575:139–143
Lin XC, Ruan XS, Lou YF, Guo XQ, Fang W (2008) Genetic similarity among cultivars of Phyllostachys pubescens. Plant Syst Evol 277:67–73
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res 18:6531–6535
Sarla N, Bobba S, Siddiq EA (2003) ISSR and SSR markers based on AG and GA repeats delineate geographically diverse Oryza nivara accessions and reveal rare alleles. CurrSci 84(5):683–690
Bhat KV (2002) Molecular data analysis. In: Proceedings of the short-term training course on molecular marker application in plant breeding, ICAR, New Delhi, 26 Sep–5 October 2002
Rohlf FJ (1993) NT-SYS-pc: Numerical taxonomy and multivariate analysis system, version 2.11W. Exteer software, Setauket
Mantel N (1967) The detection of disease clustering and generalized regression approach. Cancer Res 27:209–220
Sneath PHA, Sokal RR (1973) Numerical taxonomy. Freeman Press, San Francisco
Li A, Ge S (2001) Genetic variation and clonal diversity of Psammochloa villosa (Poaceae) detected by ISSR markers. Ann Bot 87:585–590
Esselman EJ, Li JQ, Crawford D, Winduss JL, Wolfe AD (1999) Clonal diversity in the rare Calamagrostis porteri ssp. Insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Mol Ecol 8:443–451
Parsons BJ, Newbury HJ, Jackson MT, Ford-Lloyd BV (1997) Contrasting genetic diversity relationships are revealed in rice (Oryza sativa L.) using different marker types. Mol Breed 3:115–125
Eevera T, Rajandran K, Saradha S, Lashmi A (2008) Ananlysis of genetic variation in selected bamboo species using RAPD. Tree For Sci Biotechnol 2(1):54–56
Bachmann K (1997) Nuclear DNA markers in plant biosystematics research. Opera Bot 132:137–148
Landergott U, Holderegger R, Kozlowski G, Schneller JJ (2001) Historical bottlenecks decrease genetic diversity in natural populations of Dryopteris cristata. Heredity 87:344–355
Penner GA (1996) RAPD analysis of plant genomes. In: Jauhar PP (ed) Methods of genome analysis in plants. CRC, Boca Raton, pp 251–268
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The authors are thankful to division of Food Corporation of India, Anand, Gujarat, S. P. University, V.V. Nagar, Gujarat, Anand Agricultural University, Anand, Gujarat, Farmers of Kutch region, Gujarat and Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala for providing the different genotypes of bamboo.
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Desai, P., Gajera, B., Mankad, M. et al. Comparative assessment of genetic diversity among Indian bamboo genotypes using RAPD and ISSR markers. Mol Biol Rep 42, 1265–1273 (2015). https://doi.org/10.1007/s11033-015-3867-9
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DOI: https://doi.org/10.1007/s11033-015-3867-9