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Dissection of genetic overlap of drought and low-temperature tolerance QTLs at the germination stage using backcross introgression lines in soybean

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Abstract

Northeast of China is the main soybean production area, drought and low-temperature tolerance are both main factors involved in reducing soybean yield and limiting planting regions, the most effective way to solve this problem is to breed cultivars with drought and low-temperature tolerance. A set of the BC2F3 lines was constructed with Hongfeng 11 as recurrent parent and Harosoy as donor parent, and screened in drought and low-temperature condition at the germination stage. Related QTLs were obtained by Chi-test and ANOVA analysis with genotypic and phenotypic data. Eighteen QTLs of drought tolerance and 23 QTLs of low-temperature tolerance were detected. Among them, 12 QTLs were correlated with both drought and low-temperature tolerance, which showed a partial genetic overlap between drought and low-temperature tolerance at the germination stage in soybean. Among the 12 genetic overlap QTLs, Satt253, Satt513, Satt693, Satt240, Satt323, and Satt255 were detected by at least one method for both drought and low-temperature tolerance. Satt557, Satt452, Sat_331, Satt338, Satt271, and Satt588 were detected by only one analysis method. The QTLs detected above were significant loci for drought or low-temperature tolerance in soybean. This will play an important role in MAS for development of both drought and low-temperature tolerance variety.

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Acknowledgments

This research was supported in part by the “Transgenic Specific Technology” program (2009ZX08009-013B), the Chinese “Introducing International Super Agricultural Science and Technology” program [2006-G1(A)] and the “Public Agricultural research special funds projects” (200903003).

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Authors and Affiliations

  1. College of Agriculture, Northeast Agricultural University, Harbin, 150030, China

    Wen Bo Zhang, Da Wei Xin & Qing Shan Chen

  2. Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin, 150090, China

    Wen Bo Zhang, Hong Wei Jiang, Chun Yan Liu & Guo Hua Hu

  3. The National Research Center of Soybean Engineering and Technology, Harbin, 150050, China

    Guo Hua Hu

  4. Heilongjiang Academy of Agricultural Sciences Jiamusi Branch, Jiamusi, 150030, China

    Can Dong Li

  5. State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, 150040, China

    Wen Bo Zhang

  6. Agriculture and Animal Husbandry Sciences Research, Institute of Erdos, Inner Mongolia, 017000, China

    Peng Cheng Qiu

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  1. Wen Bo Zhang
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Correspondence to Qing Shan Chen.

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Wen Bo Zhang and Peng Cheng Qiu the co-first authors for this paper.

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Zhang, W.B., Qiu, P.C., Jiang, H.W. et al. Dissection of genetic overlap of drought and low-temperature tolerance QTLs at the germination stage using backcross introgression lines in soybean. Mol Biol Rep 39, 6087–6094 (2012). https://doi.org/10.1007/s11033-011-1423-9

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  • DOI: https://doi.org/10.1007/s11033-011-1423-9

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