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Gene-based high-density mapping of the gene rym7 conferring resistance to Barley mild mosaic virus (BaMMV)

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Abstract

Barley yellow mosaic virus (BaYMV) disease seriously affects winter barley (Hordeum vulgare L.) production. Improvement of resistance to this disease can prevent yield losses. Based on previous reports, the gene rym7 (rmm7), conferring partial resistance to BaMMV, was assigned to chromosome 1H, closely linked to the centromere. In this study, newly developed barley genomic resources were applied to saturate the genetic map at the rym7 locus. Out of a set of 350 gene-based markers of chromosome 1H, we genetically assigned 23 to the rym7 region, delimiting the resistance locus to a 9.9-cM interval close to the centromere by genetic mapping in 53 doubled haploid progeny of a bi-parental mapping population. Nine gene-derived co-dominant markers co-segregated with the resistance locus. Among these, we identified the eukaryotic translation initiation factor Hv-eIF(iso)4E. Its homolog in Arabidopsis thaliana confers resistance to potyviruses. However, sequencing the entire open reading frame of resistant and susceptible genotypes could not reveal any sequence variation in exons of the gene. These results demonstrate how to combine newly developed barley genomic resources for rapid gene-based marker saturation. As a result, several easy-to-handle co-dominant markers have been identified for marker-assisted selection of rym7 in barley breeding.

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Acknowledgments

We gratefully acknowledge Jelena Perovic (IPK) and Dörte Grau (JKI) for excellent technical assistance. The work was financed as part of the collaborative project “Plant KBBE II-ViReCrop” and was supported by a grant (FKZ.: 0315708) from the German Ministry of Education and Research (BMBF) to NS and FO.

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Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, 06466, Seeland (OT) Gatersleben, Germany

    Ping Yang, Ruonan Zhou, Andreas Graner & Nils Stein

  2. Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Straße 27, 06484, Quedlinburg, Germany

    Dragan Perovic, Antje Habekuß & Frank Ordon

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  1. Ping Yang
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  2. Dragan Perovic
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  3. Antje Habekuß
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  4. Ruonan Zhou
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  5. Andreas Graner
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  6. Frank Ordon
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  7. Nils Stein
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Correspondence to Nils Stein.

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Yang, P., Perovic, D., Habekuß, A. et al. Gene-based high-density mapping of the gene rym7 conferring resistance to Barley mild mosaic virus (BaMMV). Mol Breeding 32, 27–37 (2013). https://doi.org/10.1007/s11032-013-9842-z

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  • DOI: https://doi.org/10.1007/s11032-013-9842-z

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