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Methionine and homocysteine modulate the rate of ROS generation of isolated mitochondria in vitro

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Abstract

Dietary methionine restriction and supplementation in mammals have beneficial (antiaging) and detrimental effects respectively, which have been related to chronic modifications in the rate of mitochondrial ROS generation. However it is not known if methionine or its metabolites can have, in addition, direct effects on the rate of mitochondrial ROS production. This is studied here for the methionine cycle metabolites S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), homocysteine and methionine itself in isolated rat liver, kidney, heart, and brain mitochondria. The results show that methionine increases ROS production in liver and kidney mitochondria, homocysteine increases it in kidney and decreases it in the other three organs, and SAM and SAH have no effects. The variations in ROS production are localized at complexes I or III. These changes add to previously described chronic effects of methionine restriction and supplementation in vivo.

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Authors and Affiliations

  1. Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain

    Jose Gomez, Ines Sanchez-Roman, Alexia Gomez, Carlota Sanchez, Henar Suarez, Monica Lopez-Torres & Gustavo Barja

  2. Departamento de Fisiología Animal II, Facultad de Ciencias Biológicas, Universidad Complutense, c/Jose Antonio Novais 2, Madrid, 28040, Spain

    Gustavo Barja

Authors
  1. Jose Gomez
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  2. Ines Sanchez-Roman
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  3. Alexia Gomez
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  4. Carlota Sanchez
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  5. Henar Suarez
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  6. Monica Lopez-Torres
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  7. Gustavo Barja
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Correspondence to Gustavo Barja.

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Gomez, J., Sanchez-Roman, I., Gomez, A. et al. Methionine and homocysteine modulate the rate of ROS generation of isolated mitochondria in vitro. J Bioenerg Biomembr 43, 377–386 (2011). https://doi.org/10.1007/s10863-011-9368-1

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  • DOI: https://doi.org/10.1007/s10863-011-9368-1

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