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Role of allene oxide cyclase in the regulation of rice phenolic acids synthesis and allelopathic inhibition on barnyardgrass

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Abstract

Jasmonic acid (JA) and methyl jasmonate are important endogenous signal molecules that can induce plant resistance to biotic and abiotic stresses. Allene oxide cyclase (AOC) is a key enzyme in the biosynthesis of jasmonates. Here, in order to evaluate the correlation between AOC transcript level and the allelopathic potential of rice, gene expression of AOC was genetic operated in allelopathic rice PI312777 and non-allelopathic rice Lemont, using RNA interference (RNAi) and overexpression (OX) techniques respectively. An increase in endogenous JA was found in AOC-OX rice, and the transcript levels of phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, hydroxylase, CoA-ligases, O-methyltransferases, and cinnamoyl alcohol dehydrogenases, which are involved in phenolic acid synthesis, were also up-regulated in AOC-OX transgenic rice when compared with those of the wild type. Higher accumulation of protocatechuic acid, p-hydroxy benzoic acid, ferulic acid, benzoic acid, 4-hydroxy-3-methoxybenzoicacid, and cinnamic acid content were found in the AOC-OX transgenic line. The increase of phenolic acids in the AOC-OX transgenic lines led to enhanced inhibitory rates on barnyardgrass, whereas the reverse was true in the AOC-RNAi transgenic line. The results suggest that AOC can regulate the synthesis of rice phenolic acids and thus allelopathic inhibition on barnyardgrass.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 31300336, 31271670) and the National Research Foundation for the Doctoral Program of Higher Education of China (No. 20133515130001) and Provincial Natural Science Foundation of Fujian, China (No. 2015J01079).

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    Authors and Affiliations

    1. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 35002, People’s Republic of China

      Changxun Fang, Yan Yu, Weisi Chen, Xin Jian, Qingshui Wang, Haiping Zheng & Wenxiong Lin

    2. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou, 35002, People’s Republic of China

      Changxun Fang & Wenxiong Lin

    3. Key Laboratory of Crop Ecology and Molecular Physiology of Fujian Universities, Fujian Agriculture and Forestry University, Fuzhou, 35002, People’s Republic of China

      Changxun Fang & Wenxiong Lin

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    1. Changxun Fang
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    2. Yan Yu
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    3. Weisi Chen
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    4. Xin Jian
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    Correspondence to Wenxiong Lin.

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    Changxun Fang and Yan Yu have contributed equally to this work.

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    Fang, C., Yu, Y., Chen, W. et al. Role of allene oxide cyclase in the regulation of rice phenolic acids synthesis and allelopathic inhibition on barnyardgrass. Plant Growth Regul 79, 265–273 (2016). https://doi.org/10.1007/s10725-015-0131-1

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