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Comparative proteomic analysis of apple branches susceptible and resistant to ring rot disease

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Abstract

Ring rot disease, caused by the Botryosphaeria berengeriana f. sp. piricola pathogen, is a destructive disease for apple production. To gain further understanding about the defense mechanisms of apple branches against ring rot disease, a comparative proteomic analysis was conducted in our study. We selected two different host responses to B. berengeriana f.sp. piricola infection or challenge, and compared the different proteomes of susceptible and resistant apple branches that had or had not been inoculated with the pathogen. By using 2-DE and MALDI-TOF-TOF MS analysis, 27 differentially expressed proteins were identified in two inoculation assays. According to their function, the proteins were categorized into five classes. In total, according to these two inoculation assays, there were six differentially expressed defense-related proteins identified in the bark of susceptible and resistant hosts, including Mal d1, ASR, and SAMS, which may play key roles for the resistance mechanisms of each host against ring rot disease. We speculated that the only up-regulation of the ASR protein and the dramatic decrease of SAMS in the resistant host may be related to its better disease resistance. In addition, a total of 10 proteins exhibited opposite expression patterns in the bark of susceptible and resistant branches, and they may also be related to the different disease resistances of the two hosts. Due to the complexity of antifungal mechanisms of apple branch hosts against ring rot disease, to obtain more valuable insights about the interaction between the apple host and B. berengeriana f. sp. piricola pathogen, many further investigations will be conducted.

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Abbreviations

2-DE:

Two-dimensional electrophoresis

PR:

Pathogenesis-related

SAMS:

S-adenosylmethionine synthetase

APX:

Ascorbate peroxidase

ASR:

Abscisic stress ripening-like

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Acknowledgments

This research was financially supported by the earmarked fund for the China Agriculture Research System (CARS-28) and project of national science and technology supporting plan (2013BAD02B01). The views and opinions expressed in this article are solely those of the writers, and the funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Ministry of Agriculture, Beijing, People’s Republic of China

    Zhang Cai-xia, Tian Yi, Zhang Li-yi, Zong Ze-ran & Cong Pei-hua

  2. Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xinghai South Street No. 98, Xingcheng, 125100, Liaoning, China

    Zhang Cai-xia, Tian Yi, Zhang Li-yi, Zong Ze-ran & Cong Pei-hua

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  1. Zhang Cai-xia
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Correspondence to Cong Pei-hua.

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Cai-xia, Z., Yi, T., Li-yi, Z. et al. Comparative proteomic analysis of apple branches susceptible and resistant to ring rot disease. Eur J Plant Pathol 148, 329–341 (2017). https://doi.org/10.1007/s10658-016-1092-6

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