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Comparative analysis of pathogenesis-related protein 10 (PR10) genes between fungal resistant and susceptible peppers

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Abstract

To elucidate the functional roles of PR10 genes from two pepper species during plant-pathogen interactions, PR10 genes were isolated from fungal-resistant (Capsicum baccatum var. PBC80) and fungal-susceptible (C. annuum var. Yeoju) pepper fruits infected with anthracnose fungus (Colletotrichum acutatum). Despite strong nucleotide sequence identity, there were significant differences in the patterns of gene expression and protein accumulation between the genes from the two host species. Induced expression of the PR10 mRNA in PBC80 (bacPR10) was highly maintained from 24 h after infection (HAI) rather than that in Yeoju (annPR10). These mRNA expression patterns were correlated with the level of respective protein that was detected as two or three bands in each species. Substantial induction of bacPR10 proteins was confirmed by 2D-gel analysis followed by immunoblotting. Immunolocalization study showed that deposition of bacPR10 was exclusively observed in the pericarp of PBC80 fruits after fungal infection, suggesting functional significance in defence. Additionally, in vitro analysis of the enzymatic properties of PR10 proteins revealed that recombinant bacPR10 had higher ribonucleolytic activity and exhibited less sensitivity to proteinase treatment than did annPR10. Taken together, these results support the idea that relative abundance and prolonged longevity of bacPR10 in PBC80 fruits may contribute to their increased resistance in response to the anthracnose fungus, as compared with Yeoju fruit.

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Abbreviations

HAI:

Hours after infection

HR:

Hypersensitive reaction

P-loop:

Phosphate-binding loop

PR:

Pathogenesis-related

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Acknowledgments

This research was supported in part by the Priority Research Center Program through the National research Foundation (NRF) funded by the Ministry of Education, Science and Technology (2011–0018393).

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Authors and Affiliations

  1. Kumho Life & Environmental Science Laboratory, 1 Oryong-dong, Gwangju, 500-712, Korea

    Hyun Cheol Soh, Ae Ran Park & Young Soon Kim

  2. Bioenergy Research Center, Interdisciplinary Program for Bioenergy and Biomaterials of Graduate School, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Korea

    Sangkyu Park, Kyoungwhan Back & Young Soon Kim

  3. Pepper & Breeding Institute, Business Incubator, College of Agriculture & Life Science, Seoul National University, Seodun-dong, Suwon, 441-853, Korea

    Jae Bok Yoon & Hyo Guen Park

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  1. Hyun Cheol Soh
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Correspondence to Young Soon Kim.

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Soh, H.C., Park, A.R., Park, S. et al. Comparative analysis of pathogenesis-related protein 10 (PR10) genes between fungal resistant and susceptible peppers. Eur J Plant Pathol 132, 37–48 (2012). https://doi.org/10.1007/s10658-011-9846-7

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