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Anticlastogenic effect of β-glucan, extracted from Saccharomyces cerevisiae, on cultured cells exposed to ultraviolet radiation

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Abstract

β-glucan is an important polysaccharide due to its medicinal properties of stimulating the immune system and preventing chronic diseases such as cancer. The aim of the present study was to determine the anticlastogenic effect of β-glucan in cells exposed to ultraviolet radiation (UV). Chromosome aberration assay was performed in drug-metabolizing cells (HTC) and non drug-metabolizing cells (CHO-K1 and repair-deficient CHO-xrs5), using different treatment protocols. Continuous treatment (UV + β-glucan) was not effective in reducing the DNA damage only in CHO-xrs5 cells. However, the pre-treatment protocol (β-glucan before UV exposition) was effective in reducing DNA damage only in CHO-K1 cells. In post-treatment (β-glucan after UV exposition) did not show significative anticlastogenic effects, although there was a tendency toward prevention. The data suggest that β-glucan has more than one action mechanism, being capable of exerting desmutagenic as well as bio-antimutagenic action. The findings also suggest that the presence of the xenobiotic metabolizing system can reduce the chemopreventive capacity of β-glucan. Therefore, these results indicate that β-glucan from Saccharomyces cerevisiae can be used in the prevention and/or reduction of DNA damage.

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References

  • Ames BN, Durston WE, Yamasaki E, Lee FD (1973) Carcinogens are mutagens. A simple test system combining liver homogenates for activation and bacteria for detection. Natl Acad Sci USA 70:2281–2285

    Article  CAS  Google Scholar 

  • Angeli JPF, Ribeiro LR, Gonzaga MLC, Soares SA, Ricardo MPSN, Tsuboy MS, Stidl R, Knasmuller S, Linhares RE, Mantovani MS (2006) Protective effects of β-glucan extracted from Agaricus brasiliensis against chemically induced DNA damage in human lymphocytes. Cell Biol Toxicol 22:285–291

    Article  CAS  Google Scholar 

  • Arvanitoyannis IS, Van Houwelingen-Koukaliaroglou M (2005) Functional foods: a survey of health claims, pros and cons, and current legislation. Crit Rev Food Sci Nutr 45:385–404

    Article  Google Scholar 

  • Cisneros RL, Gibson FC, Tzianabos AO (1996) Passive transfer of poly-(1–6)-β-glucotriosyl-(1–3)-β-glucopyranose glucan protection against lethal infection in an animal model of intra-abdominal sepsis. Infect Immun 64:2201–2205

    CAS  Google Scholar 

  • De Flora S (1998) Mechanisms of inhibitors of mutagenesis and carcinogenesis. Mutat Res 402:151–158

    Article  Google Scholar 

  • Ferrari CKB (2001) Oxidative stress pathophysiology searching for an effective antioxidant protection. Int Med J 8:175–184

    CAS  Google Scholar 

  • Ferrari CKB, Torres EAFS (2002) New dietetic compounds with anticarcinogenic properties. Rev Bras Canc 48:375–382

    Google Scholar 

  • Flagg EW, Coates RJ, Greenberg RS (1995) Epidemiologic studies of antioxidants and cancer in humans. J Am Coll Nutr 14:419–427

    CAS  Google Scholar 

  • Kada T, Shimoi K (1987) Desmutgens and bio-antimutagens: their modes of action. Bioessays 7:113–116

    Article  CAS  Google Scholar 

  • Kada T, Inoue T, Namiki N (1982) Environmental desmutagens and antidesmutagens. In: Klekowski EJ (ed) Environmental mutagens and plant biology. Praeger, New York, pp 137–151

    Google Scholar 

  • Kim YW, Kim KH, Choi HJ, Lee DS (2005) Anti-diabetic activity of β-glucans and their enzymatically hydrolyzed oligosaccharides from Agaricus blazei. Biotechnol Lett 27:483–487

    Article  CAS  Google Scholar 

  • Krizkova L, Durackova Z, Sandula J, Slamenova D, Sasinkova V, Sivonova M, Krajkovic J (2003) Fungal beta-(1–3)-D-glucan derivatives exhibit high antioxidative and antimutagenic activity in vitro. Anticancer Res 23:2751–2756

    CAS  Google Scholar 

  • Kubala L, Ruzickova J, Nickova K, Sandula J, Ciz M, Lojek A (2003) The effect of (1 → 3)-β-D-glucans, carboxymethylglucan and schizophyllan on human leukocytes in vitro. Carbohydr Res 338:2835–2840

    Article  CAS  Google Scholar 

  • Kuroda Y, Hara Y (1999) Antimutagenic and anticarcinogenic activity of tea polyphenois. Mutat Res 436:69–97

    Article  CAS  Google Scholar 

  • Lin H, She Y, Cassileth B, Sirotnak F, Rundles SC (2004) Maitake beta-glucan MD-fraction enhances bone marrow colony formation and reduces doxorubicin toxicity in vitro. Int Immunopharmacol 4:91–99

    Article  CAS  Google Scholar 

  • Mantovani MS, Bellini MF, Angeli JPF, Ribeiro LR (2008) β-glucans in promoting health: prevention against mutation and cancer. Mutat Res 658:154–161

    Article  CAS  Google Scholar 

  • Oliveira RJ, Ribeiro LR, Silva AF, Matuo R, Mantovani MS (2006) Evaluation of antimutagenic activity and mechanisms of action of β-glucan from barley, in CHO-K1 and HTC cell lines using the micronucleus test. Toxicol In Vitro 20:1225–1233

    Article  CAS  Google Scholar 

  • Oliveira RJ, Matuo R, Silva AF, Matiazi HJ, Mantovani MS, Ribeiro LR (2007) Protective effect of beta-glucan extracted from Saccharomyces cerevisiae, against DNA damage and cytotoxicity in wild-type (k1) and repair deficient (xrs5) CHO cells. Toxicol In Vitro 21:41–52

    Article  CAS  Google Scholar 

  • Riccardi G, Capaldo B, Vaccaro O (2005) Functional foods in the management of obesity and type 2 diabetes. Curr Opin Clin Nutr Metab Care 8:630–635

    Article  CAS  Google Scholar 

  • Riezzo G, Chiloiro M, Russo F (2005) Functional Foods: Salient Features and Clinical Applications. Immune Endocr Metabol Disord 5:331–337

    Article  CAS  Google Scholar 

  • Shimizu T (2010) The role of macrophage migration inhibitory factor (MIF) in ultraviolet radiation-induced carcinogenesis. Cancers 2:1555–1564

    Article  CAS  Google Scholar 

  • Sinha RP, Häder D-P (2002) UV-induced DNA damage and repair: a review. Photochem Photobiol Sci 1:225–236

    Article  CAS  Google Scholar 

  • Slameñova D, Labaj J, Krizkova L, Kogan G, Sandula J, Bresgen N, Eckl P (2003) Protective effects of fungal (1 → 3)-β-D-glucan derivatives against oxidative DNA lesions in V79 hamster lung cells. Cancer Lett 198:153–160

    Article  Google Scholar 

  • Takahashi H, Ohno N, Adachi Y, Yadomae T (2001) Association of immunological disorders in tethal side effect of NSAIDs on β-glucan-administered mice. FEMS Immunol Med Microbiol 31:1–14

    CAS  Google Scholar 

  • Tohamy AA, El-Ghor AA, El-Nahas SM, Noshy MM (2003) β-Glucan inhibits the genotoxicity of cyclophosphamide, adramycin and cisplatin. Mutat Res 541:45–53

    Article  CAS  Google Scholar 

  • Tzianabos AO, Cisneros RL (1996) Prophylaxis with the immunomodulator PGG glucan enhances antibiotic efficacy in rats infected with antibiotic-resistant bacteria. Ann NY Acad Sci 797:285–287

    Article  CAS  Google Scholar 

  • Vargas MA, Geihs MA, Maciel FE, Cruz BP, Nery LEM, Allodi S (2011) The effects of UV radiation on the visual system of the crab Neohelice granulata: a protective role of melatonin. Comp Biochem Physiol C Toxicol Pharmacol 154:427–434

    Google Scholar 

  • Waters MD, Brady AL, Stack HF, Brockman HE (1990) Antimutagenicity profiles for some model compounds. Mutat Res 238:57–85

    Article  CAS  Google Scholar 

  • Weisburger JH (1999) Mechanisms of action of antioxidants as exemplified in vegetables, tomatoes and tea. Food Chem Toxicol 37:943–948

    Article  CAS  Google Scholar 

  • Weisburger JH (2000) Eat to live, not live to eat. Nutrition 16:767–773

    Article  CAS  Google Scholar 

  • Zhang S, Hunter DJ, Forman MR, Rosner BA, Speizer FE, Colditz GA, Manson JE, Hankison SE, Willett WC (1999) Dietary carotenoids and vitamins A, C, and E and risk of breast cancer. J Natl Cancer Inst 91:547–556

    Article  CAS  Google Scholar 

  • Zimmerman JW, Lindermuth J, Fish PA, Palace GP, Stevenson TT, Demong DE (1998) A novel carbohydrate-glycosphingolipid interaction between a β-(1–3)-glucan immunomodulator, PGG-glucan, and lactosylceramide of human leukocytes. J Biol Chem 273:22014–22020

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Fundação Araucária. We also thank Dr. A. Leyva for English language editing of the manuscript.

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Authors and Affiliations

  1. Departamento de Biologia Geral, Universidade Estadual de Londrina, Campus Universitário, Londrina, Paraná, Brazil

    Ariane Fernanda da Silva, Andressa Megumi Niwa, Gláucia Fernanda Rocha D’Epiro & Mário Sérgio Mantovani

  2. Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil

    Rodrigo Juliano Oliveira

  3. Departamento de Biologia Celular, Universidade Estadual Paulista, Rio Claro, Brazil

    Lúcia Regina Ribeiro

Authors
  1. Ariane Fernanda da Silva
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  2. Rodrigo Juliano Oliveira
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  3. Andressa Megumi Niwa
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  4. Gláucia Fernanda Rocha D’Epiro
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  5. Lúcia Regina Ribeiro
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  6. Mário Sérgio Mantovani
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Correspondence to Gláucia Fernanda Rocha D’Epiro.

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da Silva, A.F., Oliveira, R.J., Niwa, A.M. et al. Anticlastogenic effect of β-glucan, extracted from Saccharomyces cerevisiae, on cultured cells exposed to ultraviolet radiation. Cytotechnology 65, 41–48 (2013). https://doi.org/10.1007/s10616-012-9448-z

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  • DOI: https://doi.org/10.1007/s10616-012-9448-z

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