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Basic Fibroblast Growth Factor Expression is Implicated in Mesenchymal Stem Cells Response to Light-Induced Retinal Injury

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Abstract

Neurotrophic factors are involved in neuroprotection and its expression in mesenchymal stem cells (MSCs) may change during light-induced retinal injury. In this study, neurotrophic factor expression in MSCs was investigated after stimulation by supernatants of homogenized retina (SHR) from normal and light-injured rats. Conditioned media from control MSCs (CM-MSCs), MSCs stimulated by normal SHR (CM-NSHR), and MSCs stimulated by light-injured SHR (CM-ISHR) were examined regarding their ability to prevent degeneration of retinal explants. Basic fibroblast growth factor (bFGF) in MSCs was knockdown by lentivirus-mediated mRNA interference. Transfected MSCs were stimulated by SHR, and retinal preservation was reevaluated in the resultant conditioned media. We detected significant up-regulation of bFGF in CM-ISHR, accompanied by superior retinal neurotrophic effects in CM-ISHR over CM-NSHR and CM-MSCs. Down-regulation of bFGF in MSCs effectively inhibited this protective effect. Adding neutralizing antibody against bFGF to CM-ISHR also induced a similar effect. It is thus concluded that retinal injury may enhance neurotrophic factor expression in MSCs and promote the repair process. bFGF may play a critical role in MSCs’ response to retinal injury.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No. 81271026) and the Innovation Platform Foundation of Fujian Province (No. 2010Y2003).

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Ophthalmology, First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 350005, China

    Wei Xu, Xiaoting Wang, Guoxing Xu & Jian Guo

  2. Fujian Institute of Ophthalmology, Fuzhou, China

    Guoxing Xu & Jian Guo

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  1. Wei Xu
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  2. Xiaoting Wang
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  3. Guoxing Xu
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  4. Jian Guo
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Correspondence to Guoxing Xu.

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Xu, W., Wang, X., Xu, G. et al. Basic Fibroblast Growth Factor Expression is Implicated in Mesenchymal Stem Cells Response to Light-Induced Retinal Injury. Cell Mol Neurobiol 33, 1171–1179 (2013). https://doi.org/10.1007/s10571-013-9983-y

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  • DOI: https://doi.org/10.1007/s10571-013-9983-y

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