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Zinc-alleviating effects on iron-induced phytotoxicity in roots of Triticum aestivum

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Biologia Plantarum

Abstract

The mechanisms of growth inhibition and antioxidative response were investigated in wheat roots exposed to 300 μM iron together with different zinc concentrations (0, 50, and 250 μM). All Zn concentrations decreased Fe content but increased Zn content in the roots and leaves of Fe-treated seedlings. Compared with Fe stress alone, 50 or 250 μM Zn + Fe treatment stimulated root growth, and increased cell viability but decreased malondialdehyde content, which were correlated with the decreases of total and apoplastic hydrogen peroxide and superoxide anion radical (O2 ·−) content along with apoplastic hydroxyl radical content. Generation of O2 ·− in response to 10 μM diphenylene iodonium suggested that NADPH oxidase activity was lower in Zn + Fe-treated roots than in other roots. In addition, cell wallbound peroxidase, diamine oxidase, and polyamine oxidase in Fe-treated roots were insensitive to Zn addition. Further study showed the stimulation of total superoxide dismutase and glutathione reductase (GR) activities as well as apoplastic catalase, ascorbate peroxidase, and GR in Zn + Fe-stressed roots in comparison with Fe-alone-treated ones. Taken together, Zn could alleviate iron-inhibitory effect on root growth, which might be associated with the decrease of lipid peroxidation, the increase of cell viability and the reductions of reactive oxygen species generation.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

DAO:

diamine oxidase

DPI:

diphenylene iodonium

EDTA:

ethylenediaminetetraacetic acid

GR:

glutathione reductase

H2O2 :

hydrogen peroxide

MDA:

malondialdehyde

NADPH:

nicotinamide adenine dinucleotide

NBT:

nitrobluetetrazolium

O2 ·− :

superoxide anion radical

·OH:

hydroxyl radical

PAO:

polyamine oxidase

PBS:

phosphate buffer solution

PM:

plasma membrane

POD:

peroxidase

PVP:

polyvinylpyrrolidone

ROS:

reactive oxygen species

SOD:

superoxide dismutase

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Authors and Affiliations

  1. School of Life Science, Northwest Normal University, Lanzhou, 730070, P.R. China

    T. Ma, X. H. Duan, Y. Y. Yang & J. Yao

  2. Centre of Urban Ecology and Environmental Biotechnology, Lanzhou City University, Lanzhou, 730070, P.R. China

    T. P. Gao

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  1. T. Ma
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  2. X. H. Duan
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  3. Y. Y. Yang
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Correspondence to Y. Y. Yang.

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Acknowledgments: This work was financially supported by the National Natural Science Foundation of China (Nos. 31470464, 31360094, 31460142), and the Science and Technology Plan Project of Gansu Province (144FKCA059). The first two authors contributed equally to the paper.

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Ma, T., Duan, X.H., Yang, Y.Y. et al. Zinc-alleviating effects on iron-induced phytotoxicity in roots of Triticum aestivum . Biol Plant 61, 733–740 (2017). https://doi.org/10.1007/s10535-017-0720-0

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  • DOI: https://doi.org/10.1007/s10535-017-0720-0

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