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Roles for NO and ROS signalling in pollen germination and pollen-tube elongation in Cupressus arizonica

  • Original Papers
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Biologia Plantarum

Abstract

Roles for nitric oxide (NO) and reactive oxygen species (ROS) during pollen-tube growth have been well established in angiosperms, but there remains lack of information regarding their potential signalling roles in pollen tubes in gymnosperms. Here, the pollen-tube elongation of Arizona cypress (Cupressus arizonica Greene) was investigated. Nitric oxide, ROS, and actin were detected using their respective fluorescent probes. Both NO and ROS were observed in the nuclei of generative cells and pollen-tube cells, and in the cytoplasm in the tip region. An intracellular NO content in the pollen cells was lowered using an NO scavenger or an NO-synthase inhibitor. Similarly, an endogenous ROS content in the pollen cells was lowered using an NAD(P)H oxidase inhibitor. These treatments reduced pollen germination and pollen-tube growth, and induced severe morphological abnormalities. Inhibition of NO and ROS accumulation also severely disrupted the actin cytoskeleton in the pollen tubes. These data indicate that NO and ROS had signalling roles in pollen germination and pollen-tube formation in cypress.

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Abbreviations

BK medium:

Brewbaker and Kwack liquid medium

[Ca2+]i :

intracellular Ca2+ concentration

cPTIO:

2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

DAF-FM:

4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate

DCFH2-DA:

2′7′-dichlorodihydrofluorescein diacetate

DPI:

diphenyleneiodonium chloride

L-NNA:

Nω-nitro-L-arginine

NOS:

nitric oxide synthase

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Department of Chemistry, Biology and Biotechnology, University of Perugia, I-06121, Perugia, Italy

    S. Pasqualini & L. Ederli

  2. Department of Life Sciences, University of Siena, I-53100, Siena, Italy

    M. Cresti, C. Del Casino & C. Faleri

  3. Department of Agricultural, Food and Environmental Sciences, University of Perugia, I-06121, Perugia, Italy

    G. Frenguelli & E. Tedeschini

Authors
  1. S. Pasqualini
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  2. M. Cresti
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  3. C. Del Casino
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  4. C. Faleri
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  5. G. Frenguelli
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  6. E. Tedeschini
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  7. L. Ederli
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Correspondence to S. Pasqualini.

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Pasqualini, S., Cresti, M., Del Casino, C. et al. Roles for NO and ROS signalling in pollen germination and pollen-tube elongation in Cupressus arizonica . Biol Plant 59, 735–744 (2015). https://doi.org/10.1007/s10535-015-0538-6

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  • DOI: https://doi.org/10.1007/s10535-015-0538-6

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