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Expression of a WIN/SHN-type regulator from wheat triggers disorganized proliferation in the Arabidopsis leaf cuticle

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Biologia Plantarum

Abstract

Based on information from the Arabidopsis model system, a putative transcriptional activator of cuticle formation (TaSHN1) was selected among the expressed sequence tags in wheat (Triticum aestivum L.). RT-PCR indicated the preferential expression of this gene in the basal, but not in the middle parts of wheat leaves. This leaf region is a likely site of cuticle formation in cereals. TaSHN1 was cloned and expressed in Arabidopsis, resulting in shiny leaf surfaces and the overproliferation of cuticular material as observed by electron microscopy. Unlike the Arabidopsis WAX INDUCER/SHINE1 (WIN/SHN1) gene, TaSHN1 triggered disorganized cuticular ultrastructure in the transgenic leaves, with the continuous layers replaced by large electrodense bodies embedded in amorphous lipid material. Toluidine blue staining and dark-adapted water release indicated increased cuticular permeability in TaSHN1-expressing Arabidopsis leaves. The expression of TaSHN1 resulted in a moderate decrease of the total number of stomata per unit leaf area in comparison with the wild type. Drought tolerance of Arabidopsis was unaffected by the transgene. The data indicate that this putative wheat orthologue of WIN/SHN transcription factors (TaSHN1) elicited both overlapping and new, distinctive phenotypes compared to other WIN/SHN-overexpressing plants. TaSHN1 transgenic Arabidopsis lines should provide a rich source of material for further comparative biochemical, physiological, and genetic studies.

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Abbreviations

PPDF:

photosynthetic photon flux density

RT-PCR:

reverse transcription — polymerase chain reaction

RWC:

relative water content

WIN/SHN :

WAX INDUCER/SHINE

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Authors and Affiliations

  1. Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Hungary

    K. Jäger, A. Fábián, D. Polgári & B. Barnabás

  2. Department of Plant Physiology and Plant Biochemistry, Faculty of Horticultural Science, Corvinus University of Budapest, H-1118, Budapest, Hungary

    A. Miskó, C. Deák, E. Kiss-Bába & I. Papp

Authors
  1. K. Jäger
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  2. A. Miskó
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  3. A. Fábián
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  4. C. Deák
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  5. E. Kiss-Bába
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  6. D. Polgári
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  7. B. Barnabás
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  8. I. Papp
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Correspondence to I. Papp.

Additional information

Acknowledgements: The authors wish to thank E. Kapral Bék and E. Gondos for their excellent technical assistance. The work was supported by the TÁMOP 4.2.1/B-09/1/KMR/-2010-0005, NKTH-OTKA CK80211, and NKTH-OTKA CK80274 grants from the National Development Agency of Hungary and the 00044/11 Bolyai János Research Scholarship of the Hungarian Academy of Sciences. C. Deák was financially supported by the Doctoral Council of Life Sciences of Corvinus University of Budapest.

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Jäger, K., Miskó, A., Fábián, A. et al. Expression of a WIN/SHN-type regulator from wheat triggers disorganized proliferation in the Arabidopsis leaf cuticle. Biol Plant 59, 29–36 (2015). https://doi.org/10.1007/s10535-014-0471-0

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  • DOI: https://doi.org/10.1007/s10535-014-0471-0

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