Abstract
TaCer6 was firstly cloned by rapid amplification of cDNA ends (RACE) and identified as a tissue-specific gene in wheat. To determine if environmental factors such as drought and low temperature induce TaCer6 transcription, we examined the effects of these factors on TaCer6 in two wheat cultivars. Our results demonstrated that light was essential for TaCer6 transcription, salt stress inhibited TaCer6 expression and application of salicylic acid enhanced TaCer6 transcripts accumulation. In addition, polyethylene glycol (PEG 6000) and abscisic acid increased the expression of TaCer6 more in the drought- and cold-tolerant cultivar Jinmai47 than in non-tolerant cultivar Shi4185. Low temperature increased TaCer6 transcription in Jinmai47 while decreased it in Shi4185.
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Abbreviations
- ABA:
-
abscisic acid
- KCS:
-
β-ketoacyl-CoA synthase
- ORF:
-
open reading frame
- PEG:
-
polyethylene glycol
- RACE:
-
rapid amplification of cDNA ends
- RT-PCR:
-
reverse transcription polymerase chain reaction
- SA:
-
salicylic acid
- VLCFAs:
-
very-long-chain fatty acids.
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Acknowledgments
We thank Dr. Wensheng Zhang, Dr. Jin Xu, Dr. Jun Ji, Dr. Zhiguo Wang and assistant researcher Jing Wang in the Center for Agricultural Resources Research, Institute of Genetic and Developmental Biology, Chinese Academy of Sciences for their selfless assistance. This work is supported by The High-Tech Research and Development Program of China (Grant No. 2006AA100201), the National Key Technology R&D Program of China (Grant No. 2006BAD29B02) and the Excellent Middle-aged or Young Scientists from Shandong Province (2009BSB02006) and the College Foundation from Shandong Province (J09LG82).
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Hu, X.J., Zhang, Z.B., Fu, Z.Y. et al. Significance of a β-ketoacyl-CoA synthase gene expression for wheat tolerance to adverse environments. Biol Plant 54, 575–578 (2010). https://doi.org/10.1007/s10535-010-0103-2
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DOI: https://doi.org/10.1007/s10535-010-0103-2