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Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis

  • Original Papers
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Biologia Plantarum

Abstract

Content of malondialdehyde (MDA), proline, phenolics, and saccharides was analyzed during different developmental stages of in vitro root and shoot organogenesis in saffron. The highest content of MDA, proline, and phenolics was detected in nodular calli. Significant changes were also found in the content of polysaccharides, soluble saccharides, oligosaccharides, and reducing saccharides during developmental stages. Histological investigation of nodular calli showed meristematic zones with small and densely stained cells situated at peripheral zones of calli. The meristematic zones surrounded some vascular areas from which de novo organs originated. The parenchymatic cells of inner zones of calli converted to procambium cells that produced vascular tissues.

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Abbreviations

BAP:

benzylaminopurine

DMRT:

Duncan multiple range test

MDA:

malondialdehyde

MS:

Murashige and Skoog

NAA:

α-naphthalenacetic acid

OS:

oligosaccharide

PS:

polysaccharide

RS:

reducing saccharide

SS:

soluble saccharide

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

TDZ:

thidiazuron

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Zanjan, Zanjan, 45371-38791, I.R. Iran

    E. Vatankhah

  2. Department of Plant Sciences, School of Biology, and Center of Excellence in Phylogeny of Living Organisms in Iran, College of Science, University of Tehran, Tehran, 14155-6455, I.R. Iran

    E. Vatankhah, V. Niknam & H. Ebrahimzadeh

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  1. E. Vatankhah
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Vatankhah, E., Niknam, V. & Ebrahimzadeh, H. Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis. Biol Plant 58, 201–208 (2014). https://doi.org/10.1007/s10535-013-0388-z

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  • DOI: https://doi.org/10.1007/s10535-013-0388-z

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