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Changes in enzyme activities, soluble sugars and phenols during in vitro adventitious shoot regeneration from leaflet explants of Albizia procera (Roxb.) Benth

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Abstract

Changes in nitrate reductase, i.e. NR (E.C. 1.7.1.1) activity, peroxidase (E.C. 1.11.1.14) activity, soluble sugars and phenols were monitored at various time intervals from day 0 to 60 during in vitro adventitious shoot regeneration from leaflet explants of Albizia procera (Roxb.) Benth. The explants were incubated on semi-solid Murashige and Skoog medium supplemented with 2.6 g l−1 Phytagel®, 2.5% sucrose, 10 μM BAP, 1 μM NAA and 15 μM AgNO3. NR activity, soluble sugars and phenols exhibited initial sharp rise on around day 20 followed by steep decline on day 25, whereas peroxidase activity peaked on day 50, highlighting significance of early input of nitrogen and energy and late emergence of lignification process for cellular differentiation and organization into adventitious shoot primordia. Morpho-anatomical changes in leaflets at various stages of in vitro adventitious shoot formation also followed the endogenous biochemical pattern.

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Abbreviations

BAP:

6-Benzylaminopurine

NAA:

α-Naphthalene Acetic Acid

NR:

Nitrate Reductase

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Authors and Affiliations

  1. Department of Botany, West Bengal State University, Berunanpukuria, Malikapur, Barasat, North 24 Parganas, Kolkata, 700126, India

    A. K. Sarkar

  2. Genetics and Plant Propagation Divison, Tropical Forest Research Institute, PO: RFRC, Mandla Road, Jabalpur, 482021, India

    Ekta Rai, Syed Naseer Shah, Sulochna Bouddha & S. A. Ansari

  3. Department of Biosciences, Rani Durgavati University, Jabalpur, 482011, India

    Y. K. Bansal

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Correspondence to S. A. Ansari.

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Sarkar, A.K., Rai, E., Shah, S.N. et al. Changes in enzyme activities, soluble sugars and phenols during in vitro adventitious shoot regeneration from leaflet explants of Albizia procera (Roxb.) Benth. New Forests 40, 323–334 (2010). https://doi.org/10.1007/s11056-010-9202-3

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