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Enhancing Corynebacterium glutamicum robustness by over-expressing a gene, mshA, for mycothiol glycosyltransferase

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Abstract

Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57, 85 and 33 % as compared to WT(pXMJ19) under stress by H2O2 (40 mM), methylglyoxal (5.8 mM), erythromycin (0.08 mg ml−1), streptomycin (0.005 mg ml−1), Cd2+ (0.01 mM), Mn2+ (2 mM), formic acid (0.05 %), acetic acid (0.15 %), levulinic acid (0.25 %), furfural (7.2 mM), and ethanol (10 % v/v), respectively. Increased MSH content also decreased the concentration of reactive oxygen species in the presence of the above stresses. Our results may open a new avenue for enhancing robustness of industrial bacteria for production of commodity chemicals.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 program, grant 2013AA102802), National Natural Science Foundation of China (Nos. 31170121 and 31270078) and the Opening Project of State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences (No. SKLMR-20120601).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Rep of ublicChina

    Ying-Bao Liu, Can Chen, Mei-Ru Si, Lei Zhang, Yao Wang & Xi-Hui Shen

  2. Environmental Analytical Laboratory, National Physical & Standards Laboratory, PCSIR, Islamabad, Pakistan

    Muhammad Tausif Chaudhry

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  1. Ying-Bao Liu
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  2. Can Chen
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  3. Muhammad Tausif Chaudhry
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  4. Mei-Ru Si
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  5. Lei Zhang
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  6. Yao Wang
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  7. Xi-Hui Shen
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Correspondence to Xi-Hui Shen.

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Liu, YB., Chen, C., Chaudhry, M.T. et al. Enhancing Corynebacterium glutamicum robustness by over-expressing a gene, mshA, for mycothiol glycosyltransferase. Biotechnol Lett 36, 1453–1459 (2014). https://doi.org/10.1007/s10529-014-1501-x

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  • DOI: https://doi.org/10.1007/s10529-014-1501-x

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